Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies
Activity characteristics and kinetic aspects of a cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans DF 9R were studied. A mixture of α-, β- and γ-cyclodextrins (CDs), glucose, maltose and negligible amounts of longer linear dextrins were produced from gelatinized amylose, amylopectin...
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0141022909000854 http://hdl.handle.net/20.500.12123/5950 https://doi.org/10.1016/j.enzmictec.2009.04.002 |
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| author | Szerman, Natalia Rodríguez Gastón, Jorgelina Andrea Costa, Hernán Krymkiewicz, Norberto Ferrarotti, Susana Alicia |
| author_browse | Costa, Hernán Ferrarotti, Susana Alicia Krymkiewicz, Norberto Rodríguez Gastón, Jorgelina Andrea Szerman, Natalia |
| author_facet | Szerman, Natalia Rodríguez Gastón, Jorgelina Andrea Costa, Hernán Krymkiewicz, Norberto Ferrarotti, Susana Alicia |
| author_sort | Szerman, Natalia |
| collection | INTA Digital |
| description | Activity characteristics and kinetic aspects of a cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans DF 9R were studied. A mixture of α-, β- and γ-cyclodextrins (CDs), glucose, maltose and negligible amounts of longer linear dextrins were produced from gelatinized amylose, amylopectin and starch from different sources. In the coupling reaction, CDs were the substrates in the presence of acceptors such as maltose and/or longer oligosaccharides. From oligosaccharides formed by three or more glucose units, this enzyme produced linear chains of several lengths which were then cyclized. CGTase catalytic efficiency was compared employing an analytical grade starch and cassava starch for food use. Since the results obtained were similar for both starches, the use of an economic starch is an advantage. CGTase was inhibited by the substrate and its own products. Starch concentrations over 20 mg/mL inhibited the cyclizing activity. CDs behaved as competitive inhibitors and maltose as an uncompetitive inhibitor while maltotriose showed a mixed inhibition pattern. Limit dextrins showed a scarce inhibitory effect on enzyme activity. CD production could be improved with an ultrafiltration membrane reactor for continuous removal of the products; the starch concentration should be maintained below an inhibitory concentration and limit dextrins would remain in the reactor without affecting enzyme activity. |
| format | Artículo |
| id | INTA5950 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA59502019-09-24T11:18:41Z Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies Szerman, Natalia Rodríguez Gastón, Jorgelina Andrea Costa, Hernán Krymkiewicz, Norberto Ferrarotti, Susana Alicia Cyclodextrins Glycosyltransferases Ciclodextrinas Glicosiltransferasas Bacillus circulans Cyclodextrin glycosyltransferase Kinetic parameters Ciclodextrina glicosiltransferasa Parámetros cinéticos Bacillus circulans DF 9R Activity characteristics and kinetic aspects of a cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans DF 9R were studied. A mixture of α-, β- and γ-cyclodextrins (CDs), glucose, maltose and negligible amounts of longer linear dextrins were produced from gelatinized amylose, amylopectin and starch from different sources. In the coupling reaction, CDs were the substrates in the presence of acceptors such as maltose and/or longer oligosaccharides. From oligosaccharides formed by three or more glucose units, this enzyme produced linear chains of several lengths which were then cyclized. CGTase catalytic efficiency was compared employing an analytical grade starch and cassava starch for food use. Since the results obtained were similar for both starches, the use of an economic starch is an advantage. CGTase was inhibited by the substrate and its own products. Starch concentrations over 20 mg/mL inhibited the cyclizing activity. CDs behaved as competitive inhibitors and maltose as an uncompetitive inhibitor while maltotriose showed a mixed inhibition pattern. Limit dextrins showed a scarce inhibitory effect on enzyme activity. CD production could be improved with an ultrafiltration membrane reactor for continuous removal of the products; the starch concentration should be maintained below an inhibitory concentration and limit dextrins would remain in the reactor without affecting enzyme activity. Fil: Szerman, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Rodríguez Gastón, Jorgelina Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Krymkiewicz, Norberto. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. 2019-09-24T11:05:29Z 2019-09-24T11:05:29Z 2009-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0141022909000854 http://hdl.handle.net/20.500.12123/5950 0141-0229 https://doi.org/10.1016/j.enzmictec.2009.04.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Enzyme and Microbial Technology 45 (1) : 36-41 (July 2009) |
| spellingShingle | Cyclodextrins Glycosyltransferases Ciclodextrinas Glicosiltransferasas Bacillus circulans Cyclodextrin glycosyltransferase Kinetic parameters Ciclodextrina glicosiltransferasa Parámetros cinéticos Bacillus circulans DF 9R Szerman, Natalia Rodríguez Gastón, Jorgelina Andrea Costa, Hernán Krymkiewicz, Norberto Ferrarotti, Susana Alicia Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies |
| title | Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies |
| title_full | Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies |
| title_fullStr | Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies |
| title_full_unstemmed | Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies |
| title_short | Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: Activity and kinetic studies |
| title_sort | cyclodextrin glycosyltransferase from bacillus circulans df 9r activity and kinetic studies |
| topic | Cyclodextrins Glycosyltransferases Ciclodextrinas Glicosiltransferasas Bacillus circulans Cyclodextrin glycosyltransferase Kinetic parameters Ciclodextrina glicosiltransferasa Parámetros cinéticos Bacillus circulans DF 9R |
| url | https://www.sciencedirect.com/science/article/pii/S0141022909000854 http://hdl.handle.net/20.500.12123/5950 https://doi.org/10.1016/j.enzmictec.2009.04.002 |
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