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Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods...

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Autores principales: Piccinni, Florencia Elizabeth, Ontañon, Ornella Mailén, Ghio, Silvina, Sauka, Diego Hernan, Talia, Paola Mónica, Rivarola, Maximo Lisandro, Valacco, María Pía, Campos, Eleonora
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Wiley; Society for Applied Microbiology 2019
Materias:
Cellulomonas
Glicosiltransferasas
Lignocelulosa
Glycosyltransferases
Lignocellulose
Secretome
Cazymes
GH10
Glucanases
Glycosyl Hydrolases
Xylanases
Acceso en línea:http://hdl.handle.net/20.500.12123/4769
https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176
https://doi.org/10.1111/jam.14176
Sumario:Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.

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