Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables
Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC det...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2019
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| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/pii/S0740002019304332 http://hdl.handle.net/20.500.12123/5684 https://doi.org/10.1016/j.fm.2019.103273 |
| _version_ | 1855483669121073152 |
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| author | Costa, Magdalena Sucari, Adriana Epszteyn, Sergio Oteiza, Juan Martín Gentiluomo, Jimena Melamed, Celia Figueroa, Yamila Mingorance, Santiago Emmanuel Grisaro, Agustina Spioussas, Silvia Buffoni Almeida, Mariana Caruso, Mailen Pontoni, Andrés Signorini, Marcelo Leotta, Gerardo Anibal |
| author_browse | Buffoni Almeida, Mariana Caruso, Mailen Costa, Magdalena Epszteyn, Sergio Figueroa, Yamila Gentiluomo, Jimena Grisaro, Agustina Leotta, Gerardo Anibal Melamed, Celia Mingorance, Santiago Emmanuel Oteiza, Juan Martín Pontoni, Andrés Signorini, Marcelo Spioussas, Silvia Sucari, Adriana |
| author_facet | Costa, Magdalena Sucari, Adriana Epszteyn, Sergio Oteiza, Juan Martín Gentiluomo, Jimena Melamed, Celia Figueroa, Yamila Mingorance, Santiago Emmanuel Grisaro, Agustina Spioussas, Silvia Buffoni Almeida, Mariana Caruso, Mailen Pontoni, Andrés Signorini, Marcelo Leotta, Gerardo Anibal |
| author_sort | Costa, Magdalena |
| collection | INTA Digital |
| description | Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory. |
| format | Artículo |
| id | INTA5684 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA56842019-08-26T11:57:08Z Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables Costa, Magdalena Sucari, Adriana Epszteyn, Sergio Oteiza, Juan Martín Gentiluomo, Jimena Melamed, Celia Figueroa, Yamila Mingorance, Santiago Emmanuel Grisaro, Agustina Spioussas, Silvia Buffoni Almeida, Mariana Caruso, Mailen Pontoni, Andrés Signorini, Marcelo Leotta, Gerardo Anibal Escherichia coli Toxinas Carne Hortalizas Identificación Control de Enfermedades Toxins Meat Vegetables Identification Disease Control Shiga toxin Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory. EEA Rafaela Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Epszteyn, Sergio. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina Fil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); Argentina Fil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Melamed, Celia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina Fil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Mingorance, Santiago Emmanuel. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina Fil: Grisaro, Agustina. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Spioussas, Silvia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina Fil: Buffoni Almeida, Mariana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina Fil: Caruso, Mailen. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina Fil: Pontoni, Andrés. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina 2019-08-26T11:55:22Z 2019-08-26T11:55:22Z 2019-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0740002019304332 http://hdl.handle.net/20.500.12123/5684 0740-0020 1095-9998 https://doi.org/10.1016/j.fm.2019.103273 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Food Microbiology 84 : 103273 (December 2019) |
| spellingShingle | Escherichia coli Toxinas Carne Hortalizas Identificación Control de Enfermedades Toxins Meat Vegetables Identification Disease Control Shiga toxin Costa, Magdalena Sucari, Adriana Epszteyn, Sergio Oteiza, Juan Martín Gentiluomo, Jimena Melamed, Celia Figueroa, Yamila Mingorance, Santiago Emmanuel Grisaro, Agustina Spioussas, Silvia Buffoni Almeida, Mariana Caruso, Mailen Pontoni, Andrés Signorini, Marcelo Leotta, Gerardo Anibal Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables |
| title | Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables |
| title_full | Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables |
| title_fullStr | Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables |
| title_full_unstemmed | Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables |
| title_short | Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables |
| title_sort | comparison of six commercial systems for the detection of non o157 stec in meat and vegetables |
| topic | Escherichia coli Toxinas Carne Hortalizas Identificación Control de Enfermedades Toxins Meat Vegetables Identification Disease Control Shiga toxin |
| url | https://www.sciencedirect.com/science/article/pii/S0740002019304332 http://hdl.handle.net/20.500.12123/5684 https://doi.org/10.1016/j.fm.2019.103273 |
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