Assessing Tn5 and sleeping beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencie...

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Main Authors:	Bevacqua, Romina Jimena, Fernández y Martin, Rafael, Canel, Natalia Gabriela, Gibbons, Alejandro Eduardo, Texeira, D.I.A., Lange, F., Vans Landschoot, Geraldina, Savy, Virginia, Briski, Olinda, Hiriart, María Inés, Grueso, Esther, Ivics, Zoltán, Taboga, Oscar Alberto, Kues, Wilfried A., Ferraris, S.R., Salamone, Daniel Felipe
Format:	Artículo
Language:	Inglés
Published:	2017
Subjects:	Ovinos Bovinae Cigotos Transferencia de Genes Inyección Brucelosis Sheep Zygotes Gene Transfer Injection Tn5
Online Access:	http://hdl.handle.net/20.500.12123/559 http://journals.plos.org/plosone/article/related?id=10.1371/journal.pone.0174025 https://doi.org/10.1371/journal.pone.0174025