A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle
Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and also inactivated vaccines, are currently used in many countries, they have sev...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/549 http://journal.frontiersin.org/article/10.3389/fimmu.2017.00037/full https://doi.org/10.3389/fimmu.2017.00037 |
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| author | Quattrocchi, Valeria Soria, Ivana Langellotti, Cecilia Ana Gnazzo, Victoria Gammella, Mariela Moore, Prando Dadin Zamorano, Patricia Ines |
| author_browse | Gammella, Mariela Gnazzo, Victoria Langellotti, Cecilia Ana Moore, Prando Dadin Quattrocchi, Valeria Soria, Ivana Zamorano, Patricia Ines |
| author_facet | Quattrocchi, Valeria Soria, Ivana Langellotti, Cecilia Ana Gnazzo, Victoria Gammella, Mariela Moore, Prando Dadin Zamorano, Patricia Ines |
| author_sort | Quattrocchi, Valeria |
| collection | INTA Digital |
| description | Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and also inactivated vaccines, are currently used in many countries, they have several disadvantages. DNA vaccines have emerged as an attractive approach since they have the potential to induce both humoral and cellular immune response; nevertheless, it is largely known that potency of naked DNA vaccines is limited. We demonstrated previously, in the murine model, that the use of adjuvants in combination with a DNA vaccine against BoHV-1 is immunologically beneficial.
In this study, we evaluate the immune response and protection against challenge elicited in bovines, by a DNA vaccine carrying the sequence of secreted version of glycoprotein D (gD) of BoHV-1 formulated with chemical adjuvants. Bovines were vaccinated with formulations containing the sequence of gD alone or in combination with adjuvants ESSAI 903110 or Montanide™ 1113101PR. After prime vaccination and two boosters, animals were challenged with infectious BoHV-1. Formulations containing adjuvants Montanide™ 1113101PR and ESSAI 903110 were both, capable of increasing humoral immune response against the virus and diminishing clinical symptoms. Nevertheless, only formulations containing adjuvant Montanide™ 1113101PR was capable of improving cellular immune response and diminishing viral excretion. To our knowledge, it is the first time that a BoHV-1 DNA vaccine is combined with adjuvants and tested in cattle.
These results could be useful to design a vaccine for the control of bovine rhinotracheitis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA549 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA5492018-01-12T12:54:24Z A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle Quattrocchi, Valeria Soria, Ivana Langellotti, Cecilia Ana Gnazzo, Victoria Gammella, Mariela Moore, Prando Dadin Zamorano, Patricia Ines Virus herpes simplex ADN Vacuna Ganado Bovino Coadyuvantes Herpes simplex virus Vaccines Cattle Adjuvants Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and also inactivated vaccines, are currently used in many countries, they have several disadvantages. DNA vaccines have emerged as an attractive approach since they have the potential to induce both humoral and cellular immune response; nevertheless, it is largely known that potency of naked DNA vaccines is limited. We demonstrated previously, in the murine model, that the use of adjuvants in combination with a DNA vaccine against BoHV-1 is immunologically beneficial. In this study, we evaluate the immune response and protection against challenge elicited in bovines, by a DNA vaccine carrying the sequence of secreted version of glycoprotein D (gD) of BoHV-1 formulated with chemical adjuvants. Bovines were vaccinated with formulations containing the sequence of gD alone or in combination with adjuvants ESSAI 903110 or Montanide™ 1113101PR. After prime vaccination and two boosters, animals were challenged with infectious BoHV-1. Formulations containing adjuvants Montanide™ 1113101PR and ESSAI 903110 were both, capable of increasing humoral immune response against the virus and diminishing clinical symptoms. Nevertheless, only formulations containing adjuvant Montanide™ 1113101PR was capable of improving cellular immune response and diminishing viral excretion. To our knowledge, it is the first time that a BoHV-1 DNA vaccine is combined with adjuvants and tested in cattle. These results could be useful to design a vaccine for the control of bovine rhinotracheitis. Instituto de Virología Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gnazzo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina 2017-07-03T15:44:21Z 2017-07-03T15:44:21Z 2017-01-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/549 http://journal.frontiersin.org/article/10.3389/fimmu.2017.00037/full https://doi.org/10.3389/fimmu.2017.00037 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers in immunology, January 2017 |
| spellingShingle | Virus herpes simplex ADN Vacuna Ganado Bovino Coadyuvantes Herpes simplex virus Vaccines Cattle Adjuvants Quattrocchi, Valeria Soria, Ivana Langellotti, Cecilia Ana Gnazzo, Victoria Gammella, Mariela Moore, Prando Dadin Zamorano, Patricia Ines A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| title | A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| title_full | A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| title_fullStr | A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| title_full_unstemmed | A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| title_short | A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| title_sort | dna vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle |
| topic | Virus herpes simplex ADN Vacuna Ganado Bovino Coadyuvantes Herpes simplex virus Vaccines Cattle Adjuvants |
| url | http://hdl.handle.net/20.500.12123/549 http://journal.frontiersin.org/article/10.3389/fimmu.2017.00037/full https://doi.org/10.3389/fimmu.2017.00037 |
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