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Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1

Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide...

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Autores principales: Kornuta, Claudia Alejandra, Bidart, Juan Esteban, Soria, Ivana, Quattrocchi, Valeria, Gammella, Mariela, Tribulatti, María Virginia, Campetella, Oscar, Prato, Cecilia A., Carabelli, Julieta, Cheuquepan Valenzuel, Felipe Andres, Hecker, Yanina, Moore, Prando Dadin, Zamorano, Patricia Ines, Langellotti, Cecilia Ana
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Herpesviridae
Vaccine Adjuvants
Immune Response
Bovinae
Animal Viruses
Vaccines
Coadyuvante de Vacunas
Respuesta Inmunológica
Virus de los Animales
Vacuna
Murine Model
Modelo Murino
Acceso en línea:http://hdl.handle.net/20.500.12123/21500
https://www.sciencedirect.com/science/article/abs/pii/S0042682225000145
https://doi.org/10.1016/j.virol.2025.110402
Sumario:Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide™ GEL01 PR (GEL01) and introduced Galectin-8 (Gal-8), a lectin considered a novel adjuvant due to its immunostimulatory effects, into the formulation. Animals were vaccinated with pCIgD, pCIgD with Gal-8 (pCIgD-Gal-8), pCIgD with Gal-8 and GEL01 (pCIgD-Gal-8-GEL01), or the control plasmid pCIneo. The immune response was first assessed in a mouse model and then in bovines. The results showed that combining Gal-8 and GEL01 with pCIgD modulated immune responses at both the humoral and cellular levels in both animal models. This study evaluates the efficacy of a DNA vaccine with Gal-8 and GEL01 as potential adjuvants to enhance immune protection against BoAHV-1.

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