Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
Peach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compound...
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| Format: | Artículo |
| Language: | Español |
| Published: |
Oxford Academic
2019
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| Subjects: | |
| Online Access: | https://academic.oup.com/jxb/article/60/6/1823/516686 http://hdl.handle.net/20.500.12123/5323 https://doi.org/10.1093/jxb/erp055 |
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| author | Borsani, Julia Budde, Claudio Olaf Porrini, Lucía Lauxmann, Martin Alexander Lombardo, Verónica Andrea Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria |
| author_browse | Andreo, Carlos Santiago Borsani, Julia Budde, Claudio Olaf Drincovich, María Fabiana Lara, María Valeria Lauxmann, Martin Alexander Lombardo, Verónica Andrea Murray, Ricardo Ernesto Porrini, Lucía |
| author_facet | Borsani, Julia Budde, Claudio Olaf Porrini, Lucía Lauxmann, Martin Alexander Lombardo, Verónica Andrea Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria |
| author_sort | Borsani, Julia |
| collection | INTA Digital |
| description | Peach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compounds is of great relevance. Therefore, in this work, several metabolites and enzymes involved in carbon metabolism were analysed during the post-harvest ripening of peach fruit cv ‘Dixiland’. Depending on the enzyme studied, activity, protein level by western blot, or transcript level by quantitative real time-PCR were analysed. Even though sorbitol did not accumulate at a high level in relation to sucrose at harvest, it was rapidly consumed once the fruit was separated from the tree. During the ripening process, sucrose degradation was accompanied by an increase of glucose and fructose. Specific transcripts encoding neutral invertases (NIs) were up-regulated or down-regulated, indicating differential functions for each putative NI isoform. Phosphoenolpyruvate carboxylase was markedly induced, and may participate as a glycolytic shunt, since the malate level did not increase during post-harvest ripening. The fermentative pathway was highly induced, with increases in both the acetaldehyde level and the enzymes involved in this process. In addition, proteins differentially expressed during the post-harvest ripening process were also analysed. Overall, the present study identified enzymes and pathways operating during the post-harvest ripening of peach fruit, which may contribute to further identification of varieties with altered levels of enzymes/metabolites or in the evaluation of post-harvest treatments to produce fruit of better organoleptic attributes. |
| format | Artículo |
| id | INTA5323 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic |
| publisherStr | Oxford Academic |
| record_format | dspace |
| spelling | INTA53232019-06-14T14:35:10Z Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications Borsani, Julia Budde, Claudio Olaf Porrini, Lucía Lauxmann, Martin Alexander Lombardo, Verónica Andrea Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria Durazno Prunus persica Ácidos Orgánicos Maduramiento Metabolismo Maduración en Postcosecha Fisiología Poscosecha Peaches Organic Acids Ripening Metabolism Postharvest Ripening Postharvest Physiology Peach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compounds is of great relevance. Therefore, in this work, several metabolites and enzymes involved in carbon metabolism were analysed during the post-harvest ripening of peach fruit cv ‘Dixiland’. Depending on the enzyme studied, activity, protein level by western blot, or transcript level by quantitative real time-PCR were analysed. Even though sorbitol did not accumulate at a high level in relation to sucrose at harvest, it was rapidly consumed once the fruit was separated from the tree. During the ripening process, sucrose degradation was accompanied by an increase of glucose and fructose. Specific transcripts encoding neutral invertases (NIs) were up-regulated or down-regulated, indicating differential functions for each putative NI isoform. Phosphoenolpyruvate carboxylase was markedly induced, and may participate as a glycolytic shunt, since the malate level did not increase during post-harvest ripening. The fermentative pathway was highly induced, with increases in both the acetaldehyde level and the enzymes involved in this process. In addition, proteins differentially expressed during the post-harvest ripening process were also analysed. Overall, the present study identified enzymes and pathways operating during the post-harvest ripening of peach fruit, which may contribute to further identification of varieties with altered levels of enzymes/metabolites or in the evaluation of post-harvest treatments to produce fruit of better organoleptic attributes. EEA San Pedro Fil: Bosani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Porrini, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Lombardo, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Murray, Ricardo Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2019-06-14T14:26:10Z 2019-06-14T14:26:10Z 2009 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/jxb/article/60/6/1823/516686 http://hdl.handle.net/20.500.12123/5323 1460-2431e 0022-0957 https://doi.org/10.1093/jxb/erp055 spa info:eu-repo/semantics/restrictedAccess application/pdf Oxford Academic Journal of Experimental Botany 60 (6) : 1823-1837 (April 2009) |
| spellingShingle | Durazno Prunus persica Ácidos Orgánicos Maduramiento Metabolismo Maduración en Postcosecha Fisiología Poscosecha Peaches Organic Acids Ripening Metabolism Postharvest Ripening Postharvest Physiology Borsani, Julia Budde, Claudio Olaf Porrini, Lucía Lauxmann, Martin Alexander Lombardo, Verónica Andrea Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications |
| title | Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications |
| title_full | Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications |
| title_fullStr | Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications |
| title_full_unstemmed | Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications |
| title_short | Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications |
| title_sort | carbon metabolism of peach fruit after harvest changes in enzymes involved in organic acid and sugar level modifications |
| topic | Durazno Prunus persica Ácidos Orgánicos Maduramiento Metabolismo Maduración en Postcosecha Fisiología Poscosecha Peaches Organic Acids Ripening Metabolism Postharvest Ripening Postharvest Physiology |
| url | https://academic.oup.com/jxb/article/60/6/1823/516686 http://hdl.handle.net/20.500.12123/5323 https://doi.org/10.1093/jxb/erp055 |
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