Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley; Society for Applied Microbiology
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4769 https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176 https://doi.org/10.1111/jam.14176 |
| _version_ | 1855483493495078912 |
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| author | Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Ghio, Silvina Sauka, Diego Hernan Talia, Paola Mónica Rivarola, Maximo Lisandro Valacco, María Pía Campos, Eleonora |
| author_browse | Campos, Eleonora Ghio, Silvina Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Rivarola, Maximo Lisandro Sauka, Diego Hernan Talia, Paola Mónica Valacco, María Pía |
| author_facet | Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Ghio, Silvina Sauka, Diego Hernan Talia, Paola Mónica Rivarola, Maximo Lisandro Valacco, María Pía Campos, Eleonora |
| author_sort | Piccinni, Florencia Elizabeth |
| collection | INTA Digital |
| description | Aims:
Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails.
Methods and Results:
We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10.
Conclusions:
Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes.
Significance and Impact of the Study:
Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries. |
| format | Artículo |
| id | INTA4769 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley; Society for Applied Microbiology |
| publisherStr | Wiley; Society for Applied Microbiology |
| record_format | dspace |
| spelling | INTA47692019-03-28T13:22:48Z Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Ghio, Silvina Sauka, Diego Hernan Talia, Paola Mónica Rivarola, Maximo Lisandro Valacco, María Pía Campos, Eleonora Cellulomonas Glicosiltransferasas Lignocelulosa Glycosyltransferases Lignocellulose Secretome Cazymes GH10 Glucanases Glycosyl Hydrolases Xylanases Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries. Instituto de Biotecnología Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-28T13:15:20Z 2019-03-28T13:15:20Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4769 https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176 1364-5072 https://doi.org/10.1111/jam.14176 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley; Society for Applied Microbiology Journal of applied microbiology 126 (3) : 811-825. (March 2019) |
| spellingShingle | Cellulomonas Glicosiltransferasas Lignocelulosa Glycosyltransferases Lignocellulose Secretome Cazymes GH10 Glucanases Glycosyl Hydrolases Xylanases Piccinni, Florencia Elizabeth Ontañon, Ornella Mailén Ghio, Silvina Sauka, Diego Hernan Talia, Paola Mónica Rivarola, Maximo Lisandro Valacco, María Pía Campos, Eleonora Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title | Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_full | Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_fullStr | Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_full_unstemmed | Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_short | Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_sort | secretome profile of cellulomonas sp b6 growing on lignocellulosic substrates |
| topic | Cellulomonas Glicosiltransferasas Lignocelulosa Glycosyltransferases Lignocellulose Secretome Cazymes GH10 Glucanases Glycosyl Hydrolases Xylanases |
| url | http://hdl.handle.net/20.500.12123/4769 https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176 https://doi.org/10.1111/jam.14176 |
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