Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
Three begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer; Australasian Plant Pathology Society
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/4739 https://link.springer.com/article/10.1007%2Fs13313-018-0563-y https://doi.org/10.1007/s13313-018-0563-y |
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| author | Varela, Gonzalo Matías Avalos, Vera Reyna, Pablo Gastón Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_browse | Avalos, Vera Laguna, Irma Graciela Reyna, Pablo Gastón Rodriguez Pardina, Patricia Varela, Gonzalo Matías |
| author_facet | Varela, Gonzalo Matías Avalos, Vera Reyna, Pablo Gastón Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_sort | Varela, Gonzalo Matías |
| collection | INTA Digital |
| description | Three begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field samples. As some of the analysed begomovirus-infected samples did not react with any of the specific probes, we suspected that other viruses were present. DNA of two of these samples P160, and PRCA4, was amplified by rolling circle amplification (RCA), and cloned. Four clones were sequenced by primer walking and nucleotide comparisons established that clones P160SacI and P160ApaI shared 93 and 94% nucleotide identity with DNA-A and DNA-B of Sida golden mosaic Brazil virus (SiGMBRV), respectively. Clone PRCA4.6PstI had 94% sequence identity with DNA-A of Tomato mottle wrinkle virus (ToMoWV), and clone PRCA4.17PstI exhibited 95% nucleotide sequence identity with the DNA-A of Tomato yellow vein streak virus (ToYVS), indicating a mixed infection. Specific probes were designed using part of the common region of the viral genome of the newly identified begomoviruses and were used to test bean samples during the 2014–2016 growing seasons. ToMoWV was the most frequently detected virus. The incidence of the three previously characterized begomoviruses (BGMV, SbBMV and ToYSV) was also assessed, and BGMV was found to exhibit the highest incidence. The diversity of begomoviruses found in bean in our country is remarkable, since, six different species have been detected up to the present. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4739 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer; Australasian Plant Pathology Society |
| publisherStr | Springer; Australasian Plant Pathology Society |
| record_format | dspace |
| spelling | INTA47392019-03-26T12:45:06Z Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update Varela, Gonzalo Matías Avalos, Vera Reyna, Pablo Gastón Laguna, Irma Graciela Rodriguez Pardina, Patricia Frijol Virus de las Plantas Begomovirus Phaseolus Vulgaris Beans Plant Viruses Región Noroeste Argentina Tomato Mottle Wrinkle Virus Tomato Yellow Vein Streak Virus Sida Golden Mosaic Brazil Virus Three begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field samples. As some of the analysed begomovirus-infected samples did not react with any of the specific probes, we suspected that other viruses were present. DNA of two of these samples P160, and PRCA4, was amplified by rolling circle amplification (RCA), and cloned. Four clones were sequenced by primer walking and nucleotide comparisons established that clones P160SacI and P160ApaI shared 93 and 94% nucleotide identity with DNA-A and DNA-B of Sida golden mosaic Brazil virus (SiGMBRV), respectively. Clone PRCA4.6PstI had 94% sequence identity with DNA-A of Tomato mottle wrinkle virus (ToMoWV), and clone PRCA4.17PstI exhibited 95% nucleotide sequence identity with the DNA-A of Tomato yellow vein streak virus (ToYVS), indicating a mixed infection. Specific probes were designed using part of the common region of the viral genome of the newly identified begomoviruses and were used to test bean samples during the 2014–2016 growing seasons. ToMoWV was the most frequently detected virus. The incidence of the three previously characterized begomoviruses (BGMV, SbBMV and ToYSV) was also assessed, and BGMV was found to exhibit the highest incidence. The diversity of begomoviruses found in bean in our country is remarkable, since, six different species have been detected up to the present. Instituto de Patología Vegetal Fil: Varela, Gonzalo Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Avalos, Vera. Poder Judicial de Santa Cruz. Laboratorio Regional de Investigación Forense-Zona Sur; Argentina Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2019-03-26T12:38:55Z 2019-03-26T12:38:55Z 2018-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4739 https://link.springer.com/article/10.1007%2Fs13313-018-0563-y 0815-3191 1448-6032 (Online) https://doi.org/10.1007/s13313-018-0563-y eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Springer; Australasian Plant Pathology Society Australasian plant pathology 47 (4) : 343–350. (July 2018) |
| spellingShingle | Frijol Virus de las Plantas Begomovirus Phaseolus Vulgaris Beans Plant Viruses Región Noroeste Argentina Tomato Mottle Wrinkle Virus Tomato Yellow Vein Streak Virus Sida Golden Mosaic Brazil Virus Varela, Gonzalo Matías Avalos, Vera Reyna, Pablo Gastón Laguna, Irma Graciela Rodriguez Pardina, Patricia Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update |
| title | Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update |
| title_full | Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update |
| title_fullStr | Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update |
| title_full_unstemmed | Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update |
| title_short | Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update |
| title_sort | identification molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern argentina an update |
| topic | Frijol Virus de las Plantas Begomovirus Phaseolus Vulgaris Beans Plant Viruses Región Noroeste Argentina Tomato Mottle Wrinkle Virus Tomato Yellow Vein Streak Virus Sida Golden Mosaic Brazil Virus |
| url | http://hdl.handle.net/20.500.12123/4739 https://link.springer.com/article/10.1007%2Fs13313-018-0563-y https://doi.org/10.1007/s13313-018-0563-y |
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