Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and hea...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4731 https://link.springer.com/article/10.1007%2Fs11816-018-0477-8 https://doi.org/10.1007/s11816-018-0477-8 |
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| author | Kopertekh, Lilya Krebs, Erika Guzman, Fabiana |
| author_browse | Guzman, Fabiana Kopertekh, Lilya Krebs, Erika |
| author_facet | Kopertekh, Lilya Krebs, Erika Guzman, Fabiana |
| author_sort | Kopertekh, Lilya |
| collection | INTA Digital |
| description | To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well. |
| format | Artículo |
| id | INTA4731 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA47312019-03-25T18:07:04Z Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus Kopertekh, Lilya Krebs, Erika Guzman, Fabiana Cowpea Mosaic Comovirus Regulatory Sequences Potatoes Recombination Comovirus del Mosaico de Caupi Secuencia Reguladora Papa Solanum Tuberosum Recombinación Vegetative Progeny To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well. Instituto de Patología Vegetal Fil: Kopertekh, Lilya. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; Alemania Fil: Krebs, Erika. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; Alemania Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2019-03-25T18:02:03Z 2019-03-25T18:02:03Z 2018-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4731 https://link.springer.com/article/10.1007%2Fs11816-018-0477-8 1863-5466 1863-5474 (Online) https://doi.org/10.1007/s11816-018-0477-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant biotechnology reports 12 (2) : 127–137. (April 2018) |
| spellingShingle | Cowpea Mosaic Comovirus Regulatory Sequences Potatoes Recombination Comovirus del Mosaico de Caupi Secuencia Reguladora Papa Solanum Tuberosum Recombinación Vegetative Progeny Kopertekh, Lilya Krebs, Erika Guzman, Fabiana Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus |
| title | Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus |
| title_full | Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus |
| title_fullStr | Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus |
| title_full_unstemmed | Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus |
| title_short | Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus |
| title_sort | improvement of conditional cre lox system through application of the regulatory sequences from cowpea mosaic virus |
| topic | Cowpea Mosaic Comovirus Regulatory Sequences Potatoes Recombination Comovirus del Mosaico de Caupi Secuencia Reguladora Papa Solanum Tuberosum Recombinación Vegetative Progeny |
| url | http://hdl.handle.net/20.500.12123/4731 https://link.springer.com/article/10.1007%2Fs11816-018-0477-8 https://doi.org/10.1007/s11816-018-0477-8 |
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