Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Wiley
2019
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| Subjects: | |
| Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/ppl.12259 http://hdl.handle.net/20.500.12123/4653 https://doi.org/10.1111/ppl.12259 |
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| author | Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Anderson, Catalina Margarita Podesta, Florencio Esteban |
| author_browse | Anderson, Catalina Margarita Bello, Fernando Cocco, Mariángeles Meier, Guillermo Enrique Moreno, Alejandra Soledad Perotti, Valeria Elisa Podesta, Florencio Esteban Tripodi, Karina Eva Josefina Vazquez, Daniel Eduardo |
| author_facet | Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Anderson, Catalina Margarita Podesta, Florencio Esteban |
| author_sort | Perotti, Valeria Elisa |
| collection | INTA Digital |
| description | The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two‐dimensional differential gel electrophoresis to analyze exposed and non‐exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography‐mass spectrometry and liquid chromatography‐mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost‐exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold‐sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage. |
| format | Artículo |
| id | INTA4653 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA46532019-03-19T13:30:37Z Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Anderson, Catalina Margarita Podesta, Florencio Esteban Naranja Dulce Variedades Citrus Helada Estrés de Frío Metabolismo Sweet Oranges Varieties Frost Cold Stress Metabolism Variedad Valencia Proteomics The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two‐dimensional differential gel electrophoresis to analyze exposed and non‐exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography‐mass spectrometry and liquid chromatography‐mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost‐exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold‐sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage. EEA Concordia Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Anderson, Catalina Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2019-03-19T13:29:18Z 2019-03-19T13:29:18Z 2015-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/10.1111/ppl.12259 http://hdl.handle.net/20.500.12123/4653 0031-9317 1399-3054 https://doi.org/10.1111/ppl.12259 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Physiologia Plantarum 153 (3) : 337-354 (March 2015) |
| spellingShingle | Naranja Dulce Variedades Citrus Helada Estrés de Frío Metabolismo Sweet Oranges Varieties Frost Cold Stress Metabolism Variedad Valencia Proteomics Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Anderson, Catalina Margarita Podesta, Florencio Esteban Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure |
| title | Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure |
| title_full | Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure |
| title_fullStr | Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure |
| title_full_unstemmed | Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure |
| title_short | Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure |
| title_sort | proteomic and metabolomic profiling of valencia orange fruit after natural frost exposure |
| topic | Naranja Dulce Variedades Citrus Helada Estrés de Frío Metabolismo Sweet Oranges Varieties Frost Cold Stress Metabolism Variedad Valencia Proteomics |
| url | https://onlinelibrary.wiley.com/doi/10.1111/ppl.12259 http://hdl.handle.net/20.500.12123/4653 https://doi.org/10.1111/ppl.12259 |
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