Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR ampl...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4648 https://doi.org/10.1111/jph.12160 |
| Sumario: | A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito
(San Juan, Argentina) was transmitted mechanically to Prunus tomentosa
and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the
virus presence and serological relationship with D-strain isolates; ICRT-
PCR amplified a 1.2-kb fragment of the virus genome encoding the
CP-3′ nc region. The analysis of the sequence showed the presence of the
DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites
at the 3′ end. The phylogenetic relationships and multiple alignment with
PPV isolates from NCBI database indicated greatest (+98%) homology
with the D strain and close identity with MNAT1 (\AF360579) USA peach
isolate. The sequence analysed showed two amino acid mutations towards
the 5′ N-terminus of CP (the most variable region) with respect to a consensus
of PPV D-strain isolates. This is the first molecular characterization
of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese
plum from Argentina. |
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