Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR ampl...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4648 https://doi.org/10.1111/jph.12160 |
| _version_ | 1855483472801431552 |
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| author | Dal Zotto, Angelica Balzarini, Mónica Graciela Raigon, Juan Manuel Rossini, Mirta Noemi Ducasse, Daniel Adrian |
| author_browse | Balzarini, Mónica Graciela Dal Zotto, Angelica Ducasse, Daniel Adrian Raigon, Juan Manuel Rossini, Mirta Noemi |
| author_facet | Dal Zotto, Angelica Balzarini, Mónica Graciela Raigon, Juan Manuel Rossini, Mirta Noemi Ducasse, Daniel Adrian |
| author_sort | Dal Zotto, Angelica |
| collection | INTA Digital |
| description | A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito
(San Juan, Argentina) was transmitted mechanically to Prunus tomentosa
and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the
virus presence and serological relationship with D-strain isolates; ICRT-
PCR amplified a 1.2-kb fragment of the virus genome encoding the
CP-3′ nc region. The analysis of the sequence showed the presence of the
DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites
at the 3′ end. The phylogenetic relationships and multiple alignment with
PPV isolates from NCBI database indicated greatest (+98%) homology
with the D strain and close identity with MNAT1 (\AF360579) USA peach
isolate. The sequence analysed showed two amino acid mutations towards
the 5′ N-terminus of CP (the most variable region) with respect to a consensus
of PPV D-strain isolates. This is the first molecular characterization
of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese
plum from Argentina. |
| format | Artículo |
| id | INTA4648 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA46482019-03-19T12:21:35Z Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty Dal Zotto, Angelica Balzarini, Mónica Graciela Raigon, Juan Manuel Rossini, Mirta Noemi Ducasse, Daniel Adrian Prunus Salicina Virus Sharka del Ciruelo Variedades Plum Pox Virus Varieties Ciruelo Japonés Argentina Red Beauty (Variedad) Relación Filogenética Phylogenetic Relationships A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (\AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina. Instituto de Patología Vegetal Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Balzarini, Mónica Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Raigon, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan. Agencia de Extensión Rural San Martín; Argentina Fil: Rossini, Mirta Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2019-03-19T12:03:54Z 2019-03-19T12:03:54Z 2014-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4648 1439-0434 (Online) https://doi.org/10.1111/jph.12160 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Journal of phytopathology 162 (1) : 55-60. (January 2014) |
| spellingShingle | Prunus Salicina Virus Sharka del Ciruelo Variedades Plum Pox Virus Varieties Ciruelo Japonés Argentina Red Beauty (Variedad) Relación Filogenética Phylogenetic Relationships Dal Zotto, Angelica Balzarini, Mónica Graciela Raigon, Juan Manuel Rossini, Mirta Noemi Ducasse, Daniel Adrian Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty |
| title | Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty |
| title_full | Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty |
| title_fullStr | Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty |
| title_full_unstemmed | Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty |
| title_short | Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty |
| title_sort | plum pox virus in japanese plum from argentina serological detection and molecular characterization of an isolate from cv red beauty |
| topic | Prunus Salicina Virus Sharka del Ciruelo Variedades Plum Pox Virus Varieties Ciruelo Japonés Argentina Red Beauty (Variedad) Relación Filogenética Phylogenetic Relationships |
| url | http://hdl.handle.net/20.500.12123/4648 https://doi.org/10.1111/jph.12160 |
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