Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina

The “Zona Alta del Río Mendoza” (ZARM) is the major Malbec grape viticulture region of Argentina. The aim of the present study was to explore Saccharomyces cerevisiae biodiversity in ZARM vineyards. Interdelta PCR and RFLP mtDNA molecular markers were applied to differentiate S. cerevisiae strains....

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Autores principales: Mercado, Laura Analia, Sturm, Maria Elena, Rojo, Maria Cecilia, Ciklic, Ivan Francisco, Martínez, Claudio, Combina, Mariana
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0168160511005770
http://hdl.handle.net/20.500.12123/4547
https://doi.org/10.1016/j.ijfoodmicro.2011.09.026
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author Mercado, Laura Analia
Sturm, Maria Elena
Rojo, Maria Cecilia
Ciklic, Ivan Francisco
Martínez, Claudio
Combina, Mariana
author_browse Ciklic, Ivan Francisco
Combina, Mariana
Martínez, Claudio
Mercado, Laura Analia
Rojo, Maria Cecilia
Sturm, Maria Elena
author_facet Mercado, Laura Analia
Sturm, Maria Elena
Rojo, Maria Cecilia
Ciklic, Ivan Francisco
Martínez, Claudio
Combina, Mariana
author_sort Mercado, Laura Analia
collection INTA Digital
description The “Zona Alta del Río Mendoza” (ZARM) is the major Malbec grape viticulture region of Argentina. The aim of the present study was to explore Saccharomyces cerevisiae biodiversity in ZARM vineyards. Interdelta PCR and RFLP mtDNA molecular markers were applied to differentiate S. cerevisiae strains. The presence of commercial strains on ZARM vineyards was also assessed. Our results reveal a highly diverse, but genetically closely related, S. cerevisiae population (containing more than 190 molecular patterns among 590 S. cerevisiae isolates). According to the S. cerevisiae strain diversity found in vineyards, they were classified as vineyards with high and low polymorphic S. cerevisiae populations. Six vineyards showed a high polymorphic population, with more than 20 different S. cerevisiae molecular patterns. S. cerevisiae populations in these vineyards were diverse and irregularly distributed, with different strains in each vineyard site. Low polymorphic S. cerevisiae population vineyards displayed very low yeast diversity, with only 9 to 10 different S. cerevisiae strains and presence of two commercial strains widely distributed. Population diversity estimators were calculated to determine the population structure of S. cerevisiae in the ZARM vineyards. The obtained values support the hypothesis that the eight sampled subpopulations come indeed from a larger population.
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spelling INTA45472019-09-20T13:33:09Z Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina Mercado, Laura Analia Sturm, Maria Elena Rojo, Maria Cecilia Ciklic, Ivan Francisco Martínez, Claudio Combina, Mariana Saccharomyces Cerevisiae Levadura Vid Viña Biodiversidad Marcadores Genéticos Yeasts Grapevines Vineyards Biodiversity Genetic Markers Malbec Marcadores Moleculares Zona Alta del Río Mendoza, Mendoza Mendoza The “Zona Alta del Río Mendoza” (ZARM) is the major Malbec grape viticulture region of Argentina. The aim of the present study was to explore Saccharomyces cerevisiae biodiversity in ZARM vineyards. Interdelta PCR and RFLP mtDNA molecular markers were applied to differentiate S. cerevisiae strains. The presence of commercial strains on ZARM vineyards was also assessed. Our results reveal a highly diverse, but genetically closely related, S. cerevisiae population (containing more than 190 molecular patterns among 590 S. cerevisiae isolates). According to the S. cerevisiae strain diversity found in vineyards, they were classified as vineyards with high and low polymorphic S. cerevisiae populations. Six vineyards showed a high polymorphic population, with more than 20 different S. cerevisiae molecular patterns. S. cerevisiae populations in these vineyards were diverse and irregularly distributed, with different strains in each vineyard site. Low polymorphic S. cerevisiae population vineyards displayed very low yeast diversity, with only 9 to 10 different S. cerevisiae strains and presence of two commercial strains widely distributed. Population diversity estimators were calculated to determine the population structure of S. cerevisiae in the ZARM vineyards. The obtained values support the hypothesis that the eight sampled subpopulations come indeed from a larger population. EEA Mendoza Fil: Mercado, Laura Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Sturm, Maria Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Rojo, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ciklic, Ivan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Martínez, Claudio. Universidad de Santiago de Chile. Departamento de Ciencia y Tecnología de Alimentos y Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA); Chile Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-07T16:29:18Z 2019-03-07T16:29:18Z 2011-12-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0168160511005770 http://hdl.handle.net/20.500.12123/4547 0168-1605 https://doi.org/10.1016/j.ijfoodmicro.2011.09.026 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier International Journal of Food Microbiology 151 (3) : 319-326 (December 2011)
spellingShingle Saccharomyces Cerevisiae
Levadura
Vid
Viña
Biodiversidad
Marcadores Genéticos
Yeasts
Grapevines
Vineyards
Biodiversity
Genetic Markers
Malbec
Marcadores Moleculares
Zona Alta del Río Mendoza, Mendoza
Mendoza
Mercado, Laura Analia
Sturm, Maria Elena
Rojo, Maria Cecilia
Ciklic, Ivan Francisco
Martínez, Claudio
Combina, Mariana
Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
title Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
title_full Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
title_fullStr Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
title_full_unstemmed Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
title_short Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
title_sort biodiversity of saccharomyces cerevisiae populations in malbec vineyards from the zona alta del rio mendoza region in argentina
topic Saccharomyces Cerevisiae
Levadura
Vid
Viña
Biodiversidad
Marcadores Genéticos
Yeasts
Grapevines
Vineyards
Biodiversity
Genetic Markers
Malbec
Marcadores Moleculares
Zona Alta del Río Mendoza, Mendoza
Mendoza
url https://www.sciencedirect.com/science/article/pii/S0168160511005770
http://hdl.handle.net/20.500.12123/4547
https://doi.org/10.1016/j.ijfoodmicro.2011.09.026
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