Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4495 https://www.sciencedirect.com/science/article/pii/S0304401718303741?via%3Dihub https://doi.org/10.1016/j.vetpar.2018.11.007 |
| _version_ | 1855035333691834368 |
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| author | Tomazic, Mariela Luján Rodriguez, Anabel Elisa Lombardelli Joaquín Andrés Poklepovich Caride, Tomás Javier Garro, Carlos Javier Galarza, Roxana Ivon Tiranti, Karina Florin-Christensen, Mónica Schnittger, Leonhard |
| author_browse | Florin-Christensen, Mónica Galarza, Roxana Ivon Garro, Carlos Javier Lombardelli Joaquín Andrés Poklepovich Caride, Tomás Javier Rodriguez, Anabel Elisa Schnittger, Leonhard Tiranti, Karina Tomazic, Mariela Luján |
| author_facet | Tomazic, Mariela Luján Rodriguez, Anabel Elisa Lombardelli Joaquín Andrés Poklepovich Caride, Tomás Javier Garro, Carlos Javier Galarza, Roxana Ivon Tiranti, Karina Florin-Christensen, Mónica Schnittger, Leonhard |
| author_sort | Tomazic, Mariela Luján |
| collection | INTA Digital |
| description | The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylphosphatidylinositol (GPI) are implicated in host cell attachment and invasion and represent promising vaccine candidates. A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. Altogether, 14 putative GPI-anchored proteins could be determined of which CpH1 and CpSUB2 as well as GP60 were further characterized. Sequencing and comparison of GP60, CpH1, and CpSUB1 alleles amplified from different geographic isolates showed a high degree of conservation. All three antigens were recombinant expressed and immunoblotted using sera of 12 Cryptosporidium-infected calves sampled at age periods 1–11 and 12–28 days after birth. Specific antibody reactions against the studied antigens were detected in all analyzed calves, demonstrating their immunreactivity and expression, and recognition in vivo at an early stage of host infection. Besides the acknowledged GP60 vaccinogen, the presented reverse vaccinology approach reveals the additional vaccine candidates CpH1 and CpSUB1 for inclusion into a subunit vaccine formulation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4495 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA44952019-02-26T14:29:25Z Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology Tomazic, Mariela Luján Rodriguez, Anabel Elisa Lombardelli Joaquín Andrés Poklepovich Caride, Tomás Javier Garro, Carlos Javier Galarza, Roxana Ivon Tiranti, Karina Florin-Christensen, Mónica Schnittger, Leonhard Bovina Cryptosporidium Parvum Vacuna Bovinae Vaccines Reverse Vaccinology Glycosylphosphatidylinositol GPI Vaccinogen Bovine Cryptosporidiosis Vacunación Inversa The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylphosphatidylinositol (GPI) are implicated in host cell attachment and invasion and represent promising vaccine candidates. A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. Altogether, 14 putative GPI-anchored proteins could be determined of which CpH1 and CpSUB2 as well as GP60 were further characterized. Sequencing and comparison of GP60, CpH1, and CpSUB1 alleles amplified from different geographic isolates showed a high degree of conservation. All three antigens were recombinant expressed and immunoblotted using sera of 12 Cryptosporidium-infected calves sampled at age periods 1–11 and 12–28 days after birth. Specific antibody reactions against the studied antigens were detected in all analyzed calves, demonstrating their immunreactivity and expression, and recognition in vivo at an early stage of host infection. Besides the acknowledged GP60 vaccinogen, the presented reverse vaccinology approach reveals the additional vaccine candidates CpH1 and CpSUB1 for inclusion into a subunit vaccine formulation. Instituto de Patobiología Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Lombardelli Joaquín Andrés. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina Fil: Poklepovich Caride, Tomás Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Tiranti, Karina. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina 2019-02-26T13:43:24Z 2019-02-26T13:43:24Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4495 https://www.sciencedirect.com/science/article/pii/S0304401718303741?via%3Dihub 0304-4017 https://doi.org/10.1016/j.vetpar.2018.11.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary parasitology 264 : 74-78. (15 December 2018) |
| spellingShingle | Bovina Cryptosporidium Parvum Vacuna Bovinae Vaccines Reverse Vaccinology Glycosylphosphatidylinositol GPI Vaccinogen Bovine Cryptosporidiosis Vacunación Inversa Tomazic, Mariela Luján Rodriguez, Anabel Elisa Lombardelli Joaquín Andrés Poklepovich Caride, Tomás Javier Garro, Carlos Javier Galarza, Roxana Ivon Tiranti, Karina Florin-Christensen, Mónica Schnittger, Leonhard Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| title | Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| title_full | Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| title_fullStr | Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| title_full_unstemmed | Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| title_short | Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| title_sort | identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology |
| topic | Bovina Cryptosporidium Parvum Vacuna Bovinae Vaccines Reverse Vaccinology Glycosylphosphatidylinositol GPI Vaccinogen Bovine Cryptosporidiosis Vacunación Inversa |
| url | http://hdl.handle.net/20.500.12123/4495 https://www.sciencedirect.com/science/article/pii/S0304401718303741?via%3Dihub https://doi.org/10.1016/j.vetpar.2018.11.007 |
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