Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements
Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenet...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4488 https://doi.org/10.1111/tpj.14098 |
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| author | Cambiagno, Damián Alejandro Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian |
| author_browse | Alvarez, Maria Elena Asurmendi, Sebastian Cambiagno, Damián Alejandro Casati, Paula Nota, María Florencia Rius, Sebastián Pablo Zavallo, Diego |
| author_facet | Cambiagno, Damián Alejandro Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian |
| author_sort | Cambiagno, Damián Alejandro |
| collection | INTA Digital |
| description | Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenetic changes of neighboring transposable elements (TEs) (cis regulation). We analyzed whether these genes can also respond to changes in the epigenetic marks of distal pericentromeric TEs (trans regulation). We found that Arabidopsis tissues infected with Pseudomonas syringae pv. tomato (Pst) initially induced the expression of pericentromeric TEs, and then repressed it by RNA‐directed DNA methylation (RdDM). The latter response was accompanied by the accumulation of small RNAs (sRNAs) mapping to the TEs. Curiously these sRNAs also mapped to distal PRR/NLR genes, which were controlled by RdDM but remained induced in the infected tissues. Then, we used non‐infected mom1 (Morpheus’ molecule 1) mutants that expressed pericentromeric TEs to test if they lose repression of PRR/NLR genes. mom1 plants activated several PRR/NLR genes that were unlinked to MOM1‐targeted TEs, and showed enhanced resistance to Pst. Remarkably, the increased defenses of mom1 were abolished when MOM1/RdDM‐mediated pericentromeric TEs silencing was re‐established. Therefore, common sRNAs could control PRR/NLR genes and distal pericentromeric TEs and preferentially silence TEs when they are activated. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4488 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA44882019-02-25T17:47:53Z Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements Cambiagno, Damián Alejandro Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian Arabidopsis Thaliana Transposones Epigenético Transposons Epigenetics Defense Mechanisms RdDM MOM1 Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenetic changes of neighboring transposable elements (TEs) (cis regulation). We analyzed whether these genes can also respond to changes in the epigenetic marks of distal pericentromeric TEs (trans regulation). We found that Arabidopsis tissues infected with Pseudomonas syringae pv. tomato (Pst) initially induced the expression of pericentromeric TEs, and then repressed it by RNA‐directed DNA methylation (RdDM). The latter response was accompanied by the accumulation of small RNAs (sRNAs) mapping to the TEs. Curiously these sRNAs also mapped to distal PRR/NLR genes, which were controlled by RdDM but remained induced in the infected tissues. Then, we used non‐infected mom1 (Morpheus’ molecule 1) mutants that expressed pericentromeric TEs to test if they lose repression of PRR/NLR genes. mom1 plants activated several PRR/NLR genes that were unlinked to MOM1‐targeted TEs, and showed enhanced resistance to Pst. Remarkably, the increased defenses of mom1 were abolished when MOM1/RdDM‐mediated pericentromeric TEs silencing was re‐established. Therefore, common sRNAs could control PRR/NLR genes and distal pericentromeric TEs and preferentially silence TEs when they are activated. Instituto de Biotecnología Fil: Cambiagno, Damián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Nota, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rius, Sebastián Pablo. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Casati, Paula. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina 2019-02-25T17:26:22Z 2019-02-25T17:26:22Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4488 1365-313X https://doi.org/10.1111/tpj.14098 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant journal 96 (6) : 1178-1190. (December 2018) |
| spellingShingle | Arabidopsis Thaliana Transposones Epigenético Transposons Epigenetics Defense Mechanisms RdDM MOM1 Cambiagno, Damián Alejandro Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title | Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_full | Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_fullStr | Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_full_unstemmed | Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_short | Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_sort | immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| topic | Arabidopsis Thaliana Transposones Epigenético Transposons Epigenetics Defense Mechanisms RdDM MOM1 |
| url | http://hdl.handle.net/20.500.12123/4488 https://doi.org/10.1111/tpj.14098 |
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