Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants
Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity. It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in important plant processes, including defense. To analyze if this defensive func...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4457 https://www.sciencedirect.com/science/article/pii/S0168165618305492?via%3Dihub#! https://doi.org/10.1016/j.jbiotec.2018.07.017 |
| Sumario: | Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity.
It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in
important plant processes, including defense. To analyze if this defensive function may lead to disease tolerance
in lettuce, one of the most worldwide consumed leafy vegetable, we characterized three homozygous transgenic
lines overexpressing Snakin-1. They were biologically assessed by the inoculation with the fungal pathogens
Rhizoctonia solani and Sclerotinia sclerotiorum both in vitro and in planta at the greenhouse. When in vitro assays
were performed with R. solani on Petri dishes containing crude plant extracts it was confirmed that the expressed
Snakin-1 protein has antimicrobial activity. Furthermore, transgenic lines showed a better response than wild
type in in vivo challenges against R. solani both in chamber and in greenhouse. In addition, two of these lines
showed significant in vivo protection against the pathogen S. sclerotiorum in challenge assays on adult plants.
Our results show that Snakin-1 is an interesting candidate gene for the selection/breeding of lettuce plants
with increased fungal tolerance. |
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