Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants
Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity. It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in important plant processes, including defense. To analyze if this defensive func...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4457 https://www.sciencedirect.com/science/article/pii/S0168165618305492?via%3Dihub#! https://doi.org/10.1016/j.jbiotec.2018.07.017 |
| _version_ | 1855483436571033600 |
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| author | Darqui, Flavia Soledad Radonic, Laura Mabel Trotz, Paulina M. Lopez, Nilda Ester Vazquez Rovere, Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
| author_browse | Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela Lopez, Nilda Ester Radonic, Laura Mabel Trotz, Paulina M. Vazquez Rovere, Cecilia |
| author_facet | Darqui, Flavia Soledad Radonic, Laura Mabel Trotz, Paulina M. Lopez, Nilda Ester Vazquez Rovere, Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
| author_sort | Darqui, Flavia Soledad |
| collection | INTA Digital |
| description | Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity.
It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in
important plant processes, including defense. To analyze if this defensive function may lead to disease tolerance
in lettuce, one of the most worldwide consumed leafy vegetable, we characterized three homozygous transgenic
lines overexpressing Snakin-1. They were biologically assessed by the inoculation with the fungal pathogens
Rhizoctonia solani and Sclerotinia sclerotiorum both in vitro and in planta at the greenhouse. When in vitro assays
were performed with R. solani on Petri dishes containing crude plant extracts it was confirmed that the expressed
Snakin-1 protein has antimicrobial activity. Furthermore, transgenic lines showed a better response than wild
type in in vivo challenges against R. solani both in chamber and in greenhouse. In addition, two of these lines
showed significant in vivo protection against the pathogen S. sclerotiorum in challenge assays on adult plants.
Our results show that Snakin-1 is an interesting candidate gene for the selection/breeding of lettuce plants
with increased fungal tolerance. |
| format | Artículo |
| id | INTA4457 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA44572019-09-27T11:44:05Z Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants Darqui, Flavia Soledad Radonic, Laura Mabel Trotz, Paulina M. Lopez, Nilda Ester Vazquez Rovere, Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela Rhizoctonia Solani Sclerotinia Sclerotiorum Resistencia a la Enfermedad Lechugas Transgénicos Agente Antimicrobiano Hongos Patógenos Disease Resistance Lettuces Transgenics Antimicrobial Agents Pathogenic Fungi Snakin-1 Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity. It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in important plant processes, including defense. To analyze if this defensive function may lead to disease tolerance in lettuce, one of the most worldwide consumed leafy vegetable, we characterized three homozygous transgenic lines overexpressing Snakin-1. They were biologically assessed by the inoculation with the fungal pathogens Rhizoctonia solani and Sclerotinia sclerotiorum both in vitro and in planta at the greenhouse. When in vitro assays were performed with R. solani on Petri dishes containing crude plant extracts it was confirmed that the expressed Snakin-1 protein has antimicrobial activity. Furthermore, transgenic lines showed a better response than wild type in in vivo challenges against R. solani both in chamber and in greenhouse. In addition, two of these lines showed significant in vivo protection against the pathogen S. sclerotiorum in challenge assays on adult plants. Our results show that Snakin-1 is an interesting candidate gene for the selection/breeding of lettuce plants with increased fungal tolerance. Instituto de Biotecnología Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Trotz, Paulina M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lopez, Nilda Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-02-18T14:12:15Z 2019-02-18T14:12:15Z 2018-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4457 https://www.sciencedirect.com/science/article/pii/S0168165618305492?via%3Dihub#! 0168-1656 https://doi.org/10.1016/j.jbiotec.2018.07.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of biotechnology 283 : 62-69. (10 October 2018) |
| spellingShingle | Rhizoctonia Solani Sclerotinia Sclerotiorum Resistencia a la Enfermedad Lechugas Transgénicos Agente Antimicrobiano Hongos Patógenos Disease Resistance Lettuces Transgenics Antimicrobial Agents Pathogenic Fungi Snakin-1 Darqui, Flavia Soledad Radonic, Laura Mabel Trotz, Paulina M. Lopez, Nilda Ester Vazquez Rovere, Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants |
| title | Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants |
| title_full | Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants |
| title_fullStr | Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants |
| title_full_unstemmed | Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants |
| title_short | Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants |
| title_sort | potato snakin 1 gene enhances tolerance to rhizoctonia solani and sclerotinia sclerotiorum in transgenic lettuce plants |
| topic | Rhizoctonia Solani Sclerotinia Sclerotiorum Resistencia a la Enfermedad Lechugas Transgénicos Agente Antimicrobiano Hongos Patógenos Disease Resistance Lettuces Transgenics Antimicrobial Agents Pathogenic Fungi Snakin-1 |
| url | http://hdl.handle.net/20.500.12123/4457 https://www.sciencedirect.com/science/article/pii/S0168165618305492?via%3Dihub#! https://doi.org/10.1016/j.jbiotec.2018.07.017 |
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