Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
This study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at signifi...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2019
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| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/pii/S0304401714003884 http://hdl.handle.net/20.500.12123/4372 https://doi.org/10.1016/j.vetpar.2014.07.003 |
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| author | Katzer, Frank Canton, German Jose Burrels, Alison Palarea-Albaladejo, Javier Horton, Ben Bartley, Paul M. Pang, Yvonne Chianini, Francesca Innes, Elisabeth A. Benavides-Silván, Julio |
| author_browse | Bartley, Paul M. Benavides-Silván, Julio Burrels, Alison Canton, German Jose Chianini, Francesca Horton, Ben Innes, Elisabeth A. Katzer, Frank Palarea-Albaladejo, Javier Pang, Yvonne |
| author_facet | Katzer, Frank Canton, German Jose Burrels, Alison Palarea-Albaladejo, Javier Horton, Ben Bartley, Paul M. Pang, Yvonne Chianini, Francesca Innes, Elisabeth A. Benavides-Silván, Julio |
| author_sort | Katzer, Frank |
| collection | INTA Digital |
| description | This study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at significantly (p < 0.0001) lower levels in samples from the vaccinated/challenged group (0% in heart and 5.9% in skeletal muscles), when compared to the non-vaccinated/challenged animals (75% heart, 87.9% skeletal muscle). S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. Non-vaccinated/challenged animals showed more frequent lesions in muscles and central nervous system than the vaccinated animals. These results demonstrate that vaccination of lambs with the incomplete S48 T. gondii strain, can protect against establishment of tissue cysts following challenge with a complete strain of T. gondii. Consumption of undercooked meat containing T. gondii cysts is a major route of transmission to people, therefore vaccination of food animals may improve the safety of meat for human consumption. |
| format | Artículo |
| id | INTA4372 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43722019-02-01T12:18:40Z Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite Katzer, Frank Canton, German Jose Burrels, Alison Palarea-Albaladejo, Javier Horton, Ben Bartley, Paul M. Pang, Yvonne Chianini, Francesca Innes, Elisabeth A. Benavides-Silván, Julio Cordero Inmunización Toxoplasma gondii Vacuna Toxoplasmosis Zoonosis Lambs Immunization Vaccines Zoonoses This study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at significantly (p < 0.0001) lower levels in samples from the vaccinated/challenged group (0% in heart and 5.9% in skeletal muscles), when compared to the non-vaccinated/challenged animals (75% heart, 87.9% skeletal muscle). S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. Non-vaccinated/challenged animals showed more frequent lesions in muscles and central nervous system than the vaccinated animals. These results demonstrate that vaccination of lambs with the incomplete S48 T. gondii strain, can protect against establishment of tissue cysts following challenge with a complete strain of T. gondii. Consumption of undercooked meat containing T. gondii cysts is a major route of transmission to people, therefore vaccination of food animals may improve the safety of meat for human consumption. EEA Balcarce Fil: Katzer, Frank. Moredun Research Institute; Reino Unido Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Burrels, Alison. Moredun Research Institute; Reino Unido Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido Fil: Horton, Ben. Moredun Research Institute; Reino Unido Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido Fil: Benavides-Silván, Julio. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España 2019-02-01T12:16:51Z 2019-02-01T12:16:51Z 2014-09-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0304401714003884 http://hdl.handle.net/20.500.12123/4372 0304-4017 https://doi.org/10.1016/j.vetpar.2014.07.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology 205 (1–2) : 46-56 (September 2014) |
| spellingShingle | Cordero Inmunización Toxoplasma gondii Vacuna Toxoplasmosis Zoonosis Lambs Immunization Vaccines Zoonoses Katzer, Frank Canton, German Jose Burrels, Alison Palarea-Albaladejo, Javier Horton, Ben Bartley, Paul M. Pang, Yvonne Chianini, Francesca Innes, Elisabeth A. Benavides-Silván, Julio Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| title | Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| title_full | Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| title_fullStr | Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| title_full_unstemmed | Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| title_short | Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| title_sort | immunization of lambs with the s48 strain of toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite |
| topic | Cordero Inmunización Toxoplasma gondii Vacuna Toxoplasmosis Zoonosis Lambs Immunization Vaccines Zoonoses |
| url | https://www.sciencedirect.com/science/article/pii/S0304401714003884 http://hdl.handle.net/20.500.12123/4372 https://doi.org/10.1016/j.vetpar.2014.07.003 |
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