Selection of highly susceptible cell lines to Foot and Mouth disease virus infection
Different non-established cultures were examined to find those that showed high sensitivity to Foot and Mouth Disease Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and C, and were suitable to detect viral infection in samples of animal, as well as cell culture origin. The...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Scientific Research
2019
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| Online Access: | https://www.scirp.org/journal/PaperInformation.aspx?PaperID=36381 http://hdl.handle.net/20.500.12123/4277 https://doi.org/10.4236/ojvm.2013.35042 |
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| author | Zabal, Osvaldo Alfredo Fondevila, Norberto Antonio |
| author_browse | Fondevila, Norberto Antonio Zabal, Osvaldo Alfredo |
| author_facet | Zabal, Osvaldo Alfredo Fondevila, Norberto Antonio |
| author_sort | Zabal, Osvaldo Alfredo |
| collection | INTA Digital |
| description | Different non-established cultures were examined to find those that showed high sensitivity to Foot and Mouth Disease Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and C, and were suitable to detect viral infection in samples of animal, as well as cell culture origin. The level of detection in this system was up to ten times higher than in BHK21 cells, which were commonly used for FMDV isolation and production. Viral production levels in ovine kidney cultures ranged from similar to twice as high as in BHK21. Ovine kidney cultures maintained these characteristics for at least 18 passages, allowing their use as an alternative system for FMDV diagnoses. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4277 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Scientific Research |
| publisherStr | Scientific Research |
| record_format | dspace |
| spelling | INTA42772019-01-16T15:45:15Z Selection of highly susceptible cell lines to Foot and Mouth disease virus infection Zabal, Osvaldo Alfredo Fondevila, Norberto Antonio Enfermedades de los Animales Fiebre Aftosa Cultivo de Tejidos Células Ovinos Animal Diseases Foot and Mouth Disease Tissue Culture Cells Sheep Different non-established cultures were examined to find those that showed high sensitivity to Foot and Mouth Disease Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and C, and were suitable to detect viral infection in samples of animal, as well as cell culture origin. The level of detection in this system was up to ten times higher than in BHK21 cells, which were commonly used for FMDV isolation and production. Viral production levels in ovine kidney cultures ranged from similar to twice as high as in BHK21. Ovine kidney cultures maintained these characteristics for at least 18 passages, allowing their use as an alternative system for FMDV diagnoses. Instituto de Virología Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Fil: Fondevila, Norberto Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2019-01-16T15:43:45Z 2019-01-16T15:43:45Z 2013-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.scirp.org/journal/PaperInformation.aspx?PaperID=36381 http://hdl.handle.net/20.500.12123/4277 2165-3356 2165-3364 https://doi.org/10.4236/ojvm.2013.35042 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Scientific Research Open Journal of Veterinary Medicine 3 (5) : 263-266 (September 2013) |
| spellingShingle | Enfermedades de los Animales Fiebre Aftosa Cultivo de Tejidos Células Ovinos Animal Diseases Foot and Mouth Disease Tissue Culture Cells Sheep Zabal, Osvaldo Alfredo Fondevila, Norberto Antonio Selection of highly susceptible cell lines to Foot and Mouth disease virus infection |
| title | Selection of highly susceptible cell lines to Foot and Mouth disease virus infection |
| title_full | Selection of highly susceptible cell lines to Foot and Mouth disease virus infection |
| title_fullStr | Selection of highly susceptible cell lines to Foot and Mouth disease virus infection |
| title_full_unstemmed | Selection of highly susceptible cell lines to Foot and Mouth disease virus infection |
| title_short | Selection of highly susceptible cell lines to Foot and Mouth disease virus infection |
| title_sort | selection of highly susceptible cell lines to foot and mouth disease virus infection |
| topic | Enfermedades de los Animales Fiebre Aftosa Cultivo de Tejidos Células Ovinos Animal Diseases Foot and Mouth Disease Tissue Culture Cells Sheep |
| url | https://www.scirp.org/journal/PaperInformation.aspx?PaperID=36381 http://hdl.handle.net/20.500.12123/4277 https://doi.org/10.4236/ojvm.2013.35042 |
| work_keys_str_mv | AT zabalosvaldoalfredo selectionofhighlysusceptiblecelllinestofootandmouthdiseasevirusinfection AT fondevilanorbertoantonio selectionofhighlysusceptiblecelllinestofootandmouthdiseasevirusinfection |