Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion
Commercial probiotic bacteria are delivered mainly as frozen or freeze-dried cultures. However, spray drying is a lower cost technology that could be used for the production of probiotic cultures. In this work we aimed at screening among lactobacilli strains for candidates able to survive to spray d...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
|
| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0963996912002104 http://hdl.handle.net/20.500.12123/4242 https://doi.org/10.1016/j.foodres.2012.06.018 |
| _version_ | 1855035285915566080 |
|---|---|
| author | Paez, Roxana Beatriz Lavari, Luisina Vinderola, Celso Gabriel Audero, Gabriela Maria Cuatrin, Alejandra Zaritzky, Noemi Elisabet Reinheimer, Jorge Alberto |
| author_browse | Audero, Gabriela Maria Cuatrin, Alejandra Lavari, Luisina Paez, Roxana Beatriz Reinheimer, Jorge Alberto Vinderola, Celso Gabriel Zaritzky, Noemi Elisabet |
| author_facet | Paez, Roxana Beatriz Lavari, Luisina Vinderola, Celso Gabriel Audero, Gabriela Maria Cuatrin, Alejandra Zaritzky, Noemi Elisabet Reinheimer, Jorge Alberto |
| author_sort | Paez, Roxana Beatriz |
| collection | INTA Digital |
| description | Commercial probiotic bacteria are delivered mainly as frozen or freeze-dried cultures. However, spray drying is a lower cost technology that could be used for the production of probiotic cultures. In this work we aimed at screening among lactobacilli strains for candidates able to survive to spray drying and to study the effects of a preliminary mild heat treatment and different food matrices on post-drying survival and simulated gastric acid resistance. Heat resistance (survival to exposure at 60 °C for 5 min) in MRS broth or in 10% (wt/vol) skim milk was assessed in 22 strains of Lactobacillus casei, Lactobacillus paracasei, Lactobacillus acidophilus and Lactobacillus plantarum. Five strains (L. casei Nad, L. plantarum com, L. paracasei A13, L. plantarum 8329 and L. acidophilus A9) were selected for spray drying in 20% (wt/vol) skim milk and storage at 5, 25 or 37 °C for 75 days. For L.p. A13, L.p. com and L.a. A9 no differences in cell viability were observed due to spray drying. However, for L.c. Nad and L.p. 8329 cell death due to spray drying was 0.16 and 0.49 log orders CFU ml− 1 when a mild heat treatment (52 °C for 15 min) was applied and 0.85 and 0.95 log cycles, respectively, without preliminary mild heat treatment, showing that heat treatment enhanced survival to spray drying. The application of a heat treatment was effective for enhancing survival during storage of L.p. 8329, irrespective of the storage temperature and period. No significant cell loss at 5 and 25 °C was observed for L.c. Nad. For this strain, at 37 °C no cell counts of lactobacilli were observed after 30 days of storage. For L.a. A9, L.p. com and L.p. A13 a reduction in cell viability was observed along storage as temperature increased. Resistance to simulated gastrointestinal digestion was enhanced by spray drying. The application of a mild heat treatment before spray drying may enhance cell survival during storage and the resistance to gastrointestinal digestion. Spray drying might be used for enhancing cell functionality in a strain-dependant way. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4242 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA42422019-01-10T13:01:06Z Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion Paez, Roxana Beatriz Lavari, Luisina Vinderola, Celso Gabriel Audero, Gabriela Maria Cuatrin, Alejandra Zaritzky, Noemi Elisabet Reinheimer, Jorge Alberto Lactobacillus Probióticos Secado por Pulverización Tratamiento Térmico Digestión Probiotics Spray Drying Heat Treatment Digestion Commercial probiotic bacteria are delivered mainly as frozen or freeze-dried cultures. However, spray drying is a lower cost technology that could be used for the production of probiotic cultures. In this work we aimed at screening among lactobacilli strains for candidates able to survive to spray drying and to study the effects of a preliminary mild heat treatment and different food matrices on post-drying survival and simulated gastric acid resistance. Heat resistance (survival to exposure at 60 °C for 5 min) in MRS broth or in 10% (wt/vol) skim milk was assessed in 22 strains of Lactobacillus casei, Lactobacillus paracasei, Lactobacillus acidophilus and Lactobacillus plantarum. Five strains (L. casei Nad, L. plantarum com, L. paracasei A13, L. plantarum 8329 and L. acidophilus A9) were selected for spray drying in 20% (wt/vol) skim milk and storage at 5, 25 or 37 °C for 75 days. For L.p. A13, L.p. com and L.a. A9 no differences in cell viability were observed due to spray drying. However, for L.c. Nad and L.p. 8329 cell death due to spray drying was 0.16 and 0.49 log orders CFU ml− 1 when a mild heat treatment (52 °C for 15 min) was applied and 0.85 and 0.95 log cycles, respectively, without preliminary mild heat treatment, showing that heat treatment enhanced survival to spray drying. The application of a heat treatment was effective for enhancing survival during storage of L.p. 8329, irrespective of the storage temperature and period. No significant cell loss at 5 and 25 °C was observed for L.c. Nad. For this strain, at 37 °C no cell counts of lactobacilli were observed after 30 days of storage. For L.a. A9, L.p. com and L.p. A13 a reduction in cell viability was observed along storage as temperature increased. Resistance to simulated gastrointestinal digestion was enhanced by spray drying. The application of a mild heat treatment before spray drying may enhance cell survival during storage and the resistance to gastrointestinal digestion. Spray drying might be used for enhancing cell functionality in a strain-dependant way. EEA Rafaela Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina Fil: Audero, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Zaritzky, Noemi Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina 2019-01-10T12:56:59Z 2019-01-10T12:56:59Z 2012-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0963996912002104 http://hdl.handle.net/20.500.12123/4242 0963-9969 https://doi.org/10.1016/j.foodres.2012.06.018 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Food Research International 48 (2) : 748-754 (October 2012) |
| spellingShingle | Lactobacillus Probióticos Secado por Pulverización Tratamiento Térmico Digestión Probiotics Spray Drying Heat Treatment Digestion Paez, Roxana Beatriz Lavari, Luisina Vinderola, Celso Gabriel Audero, Gabriela Maria Cuatrin, Alejandra Zaritzky, Noemi Elisabet Reinheimer, Jorge Alberto Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| title | Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| title_full | Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| title_fullStr | Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| title_full_unstemmed | Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| title_short | Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| title_sort | effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion |
| topic | Lactobacillus Probióticos Secado por Pulverización Tratamiento Térmico Digestión Probiotics Spray Drying Heat Treatment Digestion |
| url | https://www.sciencedirect.com/science/article/pii/S0963996912002104 http://hdl.handle.net/20.500.12123/4242 https://doi.org/10.1016/j.foodres.2012.06.018 |
| work_keys_str_mv | AT paezroxanabeatriz effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion AT lavariluisina effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion AT vinderolacelsogabriel effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion AT auderogabrielamaria effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion AT cuatrinalejandra effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion AT zaritzkynoemielisabet effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion AT reinheimerjorgealberto effectofheattreatmentandspraydryingonlactobacilliviabilityandresistancetosimulatedgastrointestinaldigestion |