Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
Background: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing pr...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3908 https://www.sciencedirect.com/science/article/pii/S0378874116313289?via%3Dihub https://doi.org/10.1016/j.jep.2016.10.044 |
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| author | Tofiño Rivera, Adriana Patricia Ortega Cuadros, Mailen Galvis Pareja, David Andrés Jimenez Rios, Hedilka Merini, Luciano J. Martínez Pabón, María Cecilia |
| author_browse | Galvis Pareja, David Andrés Jimenez Rios, Hedilka Martínez Pabón, María Cecilia Merini, Luciano J. Ortega Cuadros, Mailen Tofiño Rivera, Adriana Patricia |
| author_facet | Tofiño Rivera, Adriana Patricia Ortega Cuadros, Mailen Galvis Pareja, David Andrés Jimenez Rios, Hedilka Merini, Luciano J. Martínez Pabón, María Cecilia |
| author_sort | Tofiño Rivera, Adriana Patricia |
| collection | INTA Digital |
| description | Background: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global
population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid
producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment
measures are not totally effective and generate adverse effects, which is why it is necessary to search for
complementary strategies for their management.
Aim: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on
eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils.
Methodology: Essential oils were extracted from plant material through steam distillation and then its chemical
composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta,
Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms.
Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay.
Results: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively,
and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans
biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of
95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the
concentrations of both essential oils showed toxicity on CHO cells during 24 h.
Conclusion: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms
and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components
and in order to guide a safe use in treating and preventing dental caries. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3908 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA39082018-11-15T17:05:54Z Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells Tofiño Rivera, Adriana Patricia Ortega Cuadros, Mailen Galvis Pareja, David Andrés Jimenez Rios, Hedilka Merini, Luciano J. Martínez Pabón, María Cecilia Cymbopogon Citratus Streptococcus Mutans Citotoxicidad Caries Dental Contaminación Biológica Aceites Esenciales Cytotoxicity Dental Caries Biological Contamination Essential Oils Lippia Alba Biofilms Background: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results: The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion: The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. EEA Anguil Fil: Tofiño Rivera, Adriana Patricia. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; Colombia Fil: Ortega Cuadros, Mailen. Universidad Popular del Cesar; Colombia Fil: Galvis Pareja, David Andrés. Universidad CES; Colombia Fil: Jimenez Rios, Hedilka. Universidad de Santander. Facultad de Salud. Grupo de Investigación Cienciaudes; Colombia Fil: Merini, Luciano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Martínez Pabón, María Cecilia. Universidad de Antioquía. Facultad de Odontología. Laboratorio de Microbiología Bucal. Grupo de Investigación en Patología Oral, Periodoncia y Cirugía Alvéolo-Dentaria; Colombia 2018-11-15T17:00:50Z 2018-11-15T17:00:50Z 2016-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3908 https://www.sciencedirect.com/science/article/pii/S0378874116313289?via%3Dihub 0378-8741 https://doi.org/10.1016/j.jep.2016.10.044 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of ethnopharmacology 194 : 749-754. (24 December 2016) |
| spellingShingle | Cymbopogon Citratus Streptococcus Mutans Citotoxicidad Caries Dental Contaminación Biológica Aceites Esenciales Cytotoxicity Dental Caries Biological Contamination Essential Oils Lippia Alba Biofilms Tofiño Rivera, Adriana Patricia Ortega Cuadros, Mailen Galvis Pareja, David Andrés Jimenez Rios, Hedilka Merini, Luciano J. Martínez Pabón, María Cecilia Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells |
| title | Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells |
| title_full | Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells |
| title_fullStr | Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells |
| title_full_unstemmed | Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells |
| title_short | Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells |
| title_sort | effect of lippia alba and cymbopogon citratus essential oils on biofilms of streptococcus mutans and cytotoxicity in cho cells |
| topic | Cymbopogon Citratus Streptococcus Mutans Citotoxicidad Caries Dental Contaminación Biológica Aceites Esenciales Cytotoxicity Dental Caries Biological Contamination Essential Oils Lippia Alba Biofilms |
| url | http://hdl.handle.net/20.500.12123/3908 https://www.sciencedirect.com/science/article/pii/S0378874116313289?via%3Dihub https://doi.org/10.1016/j.jep.2016.10.044 |
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