Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
In 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investig...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/3630 https://link.springer.com/article/10.1007%2Fs11262-018-1563-2#citeas https://doi.org/10.1007/s11262-018-1563-2 |
| _version_ | 1855483274237837312 |
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| author | Samarfard, Samira Bejerman, Nicolas Esteban Dietzgen, Ralf Georg |
| author_browse | Bejerman, Nicolas Esteban Dietzgen, Ralf Georg Samarfard, Samira |
| author_facet | Samarfard, Samira Bejerman, Nicolas Esteban Dietzgen, Ralf Georg |
| author_sort | Samarfard, Samira |
| collection | INTA Digital |
| description | In 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investigated the genetic variability of ADV based on the complete nucleoprotein (N) gene of 13 isolates from different lucerne-growing regions in Argentina. Phylogenetic and sequence identity analyses showed that all ADV isolates are closely related and have not diverged more than 1% in the N gene despite geographical separation. These data provide further evidence that ADV is new to science and emerged and spread very recently. A total of 43 single-nucleotide polymorphisms were identified between the ADV isolates studied. Analysis of N gene ORF sequence revealed a mutational bias, with more transitions than transversions. In all cases, the ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that ADV N gene is under predominantly purifying selection. |
| format | Artículo |
| id | INTA3630 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA36302018-10-18T14:31:03Z Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina Samarfard, Samira Bejerman, Nicolas Esteban Dietzgen, Ralf Georg Medicago Sativa Virus de las Plantas Plant Viruses Genetic Variation Variación Genética Cytorhabdovirus Polymorphism Polimorfismo Nucleoproteins Nucleoproteínas Alfalfa Alfalfa Dwarf Virus Phylogenetic Analysis Análisis Filogenético Single-nucleotide Polymorphism Nucleoprotein Gene Virus del Enanismo de la Alfalfa In 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investigated the genetic variability of ADV based on the complete nucleoprotein (N) gene of 13 isolates from different lucerne-growing regions in Argentina. Phylogenetic and sequence identity analyses showed that all ADV isolates are closely related and have not diverged more than 1% in the N gene despite geographical separation. These data provide further evidence that ADV is new to science and emerged and spread very recently. A total of 43 single-nucleotide polymorphisms were identified between the ADV isolates studied. Analysis of N gene ORF sequence revealed a mutational bias, with more transitions than transversions. In all cases, the ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that ADV N gene is under predominantly purifying selection. Instituto de Patología Vegetal Fil: Samarfard, Samira. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia 2018-10-18T14:27:29Z 2018-10-18T14:27:29Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3630 https://link.springer.com/article/10.1007%2Fs11262-018-1563-2#citeas 0920-8569 1572-994X (Online) https://doi.org/10.1007/s11262-018-1563-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Springer Virus genes 54 (4) : 612–615. (August 2018) |
| spellingShingle | Medicago Sativa Virus de las Plantas Plant Viruses Genetic Variation Variación Genética Cytorhabdovirus Polymorphism Polimorfismo Nucleoproteins Nucleoproteínas Alfalfa Alfalfa Dwarf Virus Phylogenetic Analysis Análisis Filogenético Single-nucleotide Polymorphism Nucleoprotein Gene Virus del Enanismo de la Alfalfa Samarfard, Samira Bejerman, Nicolas Esteban Dietzgen, Ralf Georg Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina |
| title | Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina |
| title_full | Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina |
| title_fullStr | Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina |
| title_full_unstemmed | Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina |
| title_short | Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina |
| title_sort | distribution and genetic variability of alfalfa dwarf virus a cytorhabdovirus associated with alfalfa dwarf disease in argentina |
| topic | Medicago Sativa Virus de las Plantas Plant Viruses Genetic Variation Variación Genética Cytorhabdovirus Polymorphism Polimorfismo Nucleoproteins Nucleoproteínas Alfalfa Alfalfa Dwarf Virus Phylogenetic Analysis Análisis Filogenético Single-nucleotide Polymorphism Nucleoprotein Gene Virus del Enanismo de la Alfalfa |
| url | http://hdl.handle.net/20.500.12123/3630 https://link.springer.com/article/10.1007%2Fs11262-018-1563-2#citeas https://doi.org/10.1007/s11262-018-1563-2 |
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