In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens
Among 360 isolates from the gastrointestinal tract (GIT) of broilers, eleven isolates which showed in vitro probiotic properties were identified and selected for further tests. After the in vitro screening, three strains were chosen for the in vivo study of persistence of fresh cultures and then one...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/pii/S0034528815001629 http://hdl.handle.net/20.500.12123/3010 https://doi.org/10.1016/j.rvsc.2015.05.017 |
| _version_ | 1855035067214069760 |
|---|---|
| author | Blajman, Jesica E. Gaziano, Cristian Zbrun, María Virginia Soto, Lorena Paola Astesana, Diego Martín Berisvil, Ayelén Patricia Romero Scharpen, Analía Signorini, Marcelo Frizzo, Laureano Sebastian |
| author_browse | Astesana, Diego Martín Berisvil, Ayelén Patricia Blajman, Jesica E. Frizzo, Laureano Sebastian Gaziano, Cristian Romero Scharpen, Analía Signorini, Marcelo Soto, Lorena Paola Zbrun, María Virginia |
| author_facet | Blajman, Jesica E. Gaziano, Cristian Zbrun, María Virginia Soto, Lorena Paola Astesana, Diego Martín Berisvil, Ayelén Patricia Romero Scharpen, Analía Signorini, Marcelo Frizzo, Laureano Sebastian |
| author_sort | Blajman, Jesica E. |
| collection | INTA Digital |
| description | Among 360 isolates from the gastrointestinal tract (GIT) of broilers, eleven isolates which showed in vitro probiotic properties were identified and selected for further tests. After the in vitro screening, three strains were chosen for the in vivo study of persistence of fresh cultures and then one strain was selected for the in vivo study of persistence of lyophilized culture. Lyophilized Lactobacillus salivarius DSPV 001P was capable of persisting in broilers during a complete rearing, even 28 days following cessation of administration. L. salivarius DSPV 001P administered to broilers and recovered from GIT was compared by pulsed-field gel electrophoresis (PFGE) to ensure that the same genotype was persistently identified. A combination of in vitro and in vivo screening of native lactic acid bacteria (LAB) described in this study may offer a method for selecting the most suitable strain for potential application as a broiler probiotic supplement. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3010 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30102018-08-08T12:17:33Z In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens Blajman, Jesica E. Gaziano, Cristian Zbrun, María Virginia Soto, Lorena Paola Astesana, Diego Martín Berisvil, Ayelén Patricia Romero Scharpen, Analía Signorini, Marcelo Frizzo, Laureano Sebastian Pollo de Engorde Probióticos Bacterias Acidolácticas Experimentación in Vitro Experimentación in Vivo Broiler Chickens Probiotics Lactic Acid Bacteria In Vitro Experimentation In Vivo Experimentation Among 360 isolates from the gastrointestinal tract (GIT) of broilers, eleven isolates which showed in vitro probiotic properties were identified and selected for further tests. After the in vitro screening, three strains were chosen for the in vivo study of persistence of fresh cultures and then one strain was selected for the in vivo study of persistence of lyophilized culture. Lyophilized Lactobacillus salivarius DSPV 001P was capable of persisting in broilers during a complete rearing, even 28 days following cessation of administration. L. salivarius DSPV 001P administered to broilers and recovered from GIT was compared by pulsed-field gel electrophoresis (PFGE) to ensure that the same genotype was persistently identified. A combination of in vitro and in vivo screening of native lactic acid bacteria (LAB) described in this study may offer a method for selecting the most suitable strain for potential application as a broiler probiotic supplement. EEA Rafaela Fil: Blajman, Jesica E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Gaziano, Cristian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Astesana, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Berisvil, Ayelén Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Romero Scharpen, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina 2018-08-08T12:16:05Z 2018-08-08T12:16:05Z 2015-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0034528815001629 http://hdl.handle.net/20.500.12123/3010 0034-5288 https://doi.org/10.1016/j.rvsc.2015.05.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Research in Veterinary Science 101 : 50-56 (August 2015) |
| spellingShingle | Pollo de Engorde Probióticos Bacterias Acidolácticas Experimentación in Vitro Experimentación in Vivo Broiler Chickens Probiotics Lactic Acid Bacteria In Vitro Experimentation In Vivo Experimentation Blajman, Jesica E. Gaziano, Cristian Zbrun, María Virginia Soto, Lorena Paola Astesana, Diego Martín Berisvil, Ayelén Patricia Romero Scharpen, Analía Signorini, Marcelo Frizzo, Laureano Sebastian In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| title | In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| title_full | In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| title_fullStr | In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| title_full_unstemmed | In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| title_short | In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| title_sort | in vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens |
| topic | Pollo de Engorde Probióticos Bacterias Acidolácticas Experimentación in Vitro Experimentación in Vivo Broiler Chickens Probiotics Lactic Acid Bacteria In Vitro Experimentation In Vivo Experimentation |
| url | https://www.sciencedirect.com/science/article/pii/S0034528815001629 http://hdl.handle.net/20.500.12123/3010 https://doi.org/10.1016/j.rvsc.2015.05.017 |
| work_keys_str_mv | AT blajmanjesicae invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT gazianocristian invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT zbrunmariavirginia invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT sotolorenapaola invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT astesanadiegomartin invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT berisvilayelenpatricia invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT romeroscharpenanalia invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT signorinimarcelo invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens AT frizzolaureanosebastian invitroandinvivoscreeningofnativelacticacidbacteriatowardtheirselectionasaprobioticinbroilerchickens |