Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion
The cost-efficient degradation of xylan to fermentable sugars is of particular interest in second generation bioethanol production, feed, food, and pulp and paper industries. Multiple potentially secreted enzymes involved in polysaccharide deconstruction are encoded in the genome of Paenibacillus sp...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2913 https://link.springer.com/article/10.1007%2Fs12155-017-9887-7 https://doi.org/10.1007/s12155-017-9887-7 |
| _version_ | 1855035049761570816 |
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| author | Ghio, Silvina Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Marrero Diaz De Vill, Rubén Talia, Paola Mónica Grasso, Daniel Horacio Campos, Eleonora |
| author_browse | Campos, Eleonora Ghio, Silvina Grasso, Daniel Horacio Marrero Diaz De Vill, Rubén Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Talia, Paola Mónica |
| author_facet | Ghio, Silvina Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Marrero Diaz De Vill, Rubén Talia, Paola Mónica Grasso, Daniel Horacio Campos, Eleonora |
| author_sort | Ghio, Silvina |
| collection | INTA Digital |
| description | The cost-efficient degradation of xylan to fermentable sugars is of particular interest in second generation bioethanol production, feed, food, and pulp and paper industries. Multiple potentially secreted enzymes involved in polysaccharide deconstruction are encoded in the genome of Paenibacillus sp. A59, a xylanolytic soil bacterium, such as three endoxylanases, seven GH43 β-xylosidases, and two GH30 glucuronoxylanases. In secretome analysis of xylan cultures, ten glycoside hydrolases were identified, including the three predicted endoxylanases, confirming their active role. The two uni-modular xylanases, a 32-KDa GH10 and a 20-KDa GH11, were recombinantly expressed and their activity on xylan was confirmed (106 and 85 IU/mg, respectively), with differences in their activity pattern. Both endoxylanases released mainly xylobiose (X2) and xylotriose (X3) from xylan and pre-treated biomasses (wheat straw, barley straw, and sweet corn cob), although only rGH10XynA released xylose (X1). rGH10XynA presented optimal conditions at pH 6, with thermal stability at 45–50 °C, while rGH11XynB showed activity in a wider range of pH, from 5 to 9, and was thermostable only at 45 °C. Moreover, GH11XynB presented sigmoidal kinetics on xylan, indicating possible cooperative binding, which was further supported by the structural model. This study provides a detailed analysis of the complete set of carbohydrate-active enzymes encoded in Paenibacillus sp. A59 genome and those effectively implicated in hemicellulose hydrolysis, contributing to understanding the mechanisms necessary for the bioconversion of this polysaccharide. Moreover, the two main free secreted xylanases, rGH10XynA and rGH11XynB, were fully characterized, supporting their potential application in industrial bioprocesses on lignocellulosic biomass. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2913 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA29132018-07-30T13:51:11Z Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion Ghio, Silvina Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Marrero Diaz De Vill, Rubén Talia, Paola Mónica Grasso, Daniel Horacio Campos, Eleonora Paenibacillus Bioconversión Biomasa Biomass Bioconversion Endoxylanases GH10 GH11 The cost-efficient degradation of xylan to fermentable sugars is of particular interest in second generation bioethanol production, feed, food, and pulp and paper industries. Multiple potentially secreted enzymes involved in polysaccharide deconstruction are encoded in the genome of Paenibacillus sp. A59, a xylanolytic soil bacterium, such as three endoxylanases, seven GH43 β-xylosidases, and two GH30 glucuronoxylanases. In secretome analysis of xylan cultures, ten glycoside hydrolases were identified, including the three predicted endoxylanases, confirming their active role. The two uni-modular xylanases, a 32-KDa GH10 and a 20-KDa GH11, were recombinantly expressed and their activity on xylan was confirmed (106 and 85 IU/mg, respectively), with differences in their activity pattern. Both endoxylanases released mainly xylobiose (X2) and xylotriose (X3) from xylan and pre-treated biomasses (wheat straw, barley straw, and sweet corn cob), although only rGH10XynA released xylose (X1). rGH10XynA presented optimal conditions at pH 6, with thermal stability at 45–50 °C, while rGH11XynB showed activity in a wider range of pH, from 5 to 9, and was thermostable only at 45 °C. Moreover, GH11XynB presented sigmoidal kinetics on xylan, indicating possible cooperative binding, which was further supported by the structural model. This study provides a detailed analysis of the complete set of carbohydrate-active enzymes encoded in Paenibacillus sp. A59 genome and those effectively implicated in hemicellulose hydrolysis, contributing to understanding the mechanisms necessary for the bioconversion of this polysaccharide. Moreover, the two main free secreted xylanases, rGH10XynA and rGH11XynB, were fully characterized, supporting their potential application in industrial bioprocesses on lignocellulosic biomass. Instituto de Suelos Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina Fil: Ontañon, Ornella Mailén. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marrero Diaz de Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Grasso, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-07-30T13:42:37Z 2018-07-30T13:42:37Z 2018-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2913 https://link.springer.com/article/10.1007%2Fs12155-017-9887-7 1939-1234 https://doi.org/10.1007/s12155-017-9887-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer BioEnergy research 11 (1) : 174–190. (March 2018) |
| spellingShingle | Paenibacillus Bioconversión Biomasa Biomass Bioconversion Endoxylanases GH10 GH11 Ghio, Silvina Ontañon, Ornella Mailén Piccinni, Florencia Elizabeth Marrero Diaz De Vill, Rubén Talia, Paola Mónica Grasso, Daniel Horacio Campos, Eleonora Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion |
| title | Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion |
| title_full | Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion |
| title_fullStr | Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion |
| title_full_unstemmed | Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion |
| title_short | Paenibacillus sp. A59 GH10 and GH11 extracellular endoxylanases: application in biomass bioconversion |
| title_sort | paenibacillus sp a59 gh10 and gh11 extracellular endoxylanases application in biomass bioconversion |
| topic | Paenibacillus Bioconversión Biomasa Biomass Bioconversion Endoxylanases GH10 GH11 |
| url | http://hdl.handle.net/20.500.12123/2913 https://link.springer.com/article/10.1007%2Fs12155-017-9887-7 https://doi.org/10.1007/s12155-017-9887-7 |
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