A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its comme...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2883 https://www.sciencedirect.com/science/article/pii/S0304423814007055 https://doi.org/10.1016/j.scienta.2014.12.032 |
| _version_ | 1855483138319319040 |
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| author | Gonzalez Roca, Lucia Iannicelli, Jesica Coviella, Maria Andrea Bugallo, Veronica Lucia Bologna, Paula Pitta Alvarez, Sandra Irene Escandon, Alejandro Salvio |
| author_browse | Bologna, Paula Bugallo, Veronica Lucia Coviella, Maria Andrea Escandon, Alejandro Salvio Gonzalez Roca, Lucia Iannicelli, Jesica Pitta Alvarez, Sandra Irene |
| author_facet | Gonzalez Roca, Lucia Iannicelli, Jesica Coviella, Maria Andrea Bugallo, Veronica Lucia Bologna, Paula Pitta Alvarez, Sandra Irene Escandon, Alejandro Salvio |
| author_sort | Gonzalez Roca, Lucia |
| collection | INTA Digital |
| description | The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program. |
| format | Artículo |
| id | INTA2883 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28832025-07-25T09:58:40Z A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) Gonzalez Roca, Lucia Iannicelli, Jesica Coviella, Maria Andrea Bugallo, Veronica Lucia Bologna, Paula Pitta Alvarez, Sandra Irene Escandon, Alejandro Salvio Verbenaceae Experimentación in Vitro Poliploidia Inducida Propagación Vegetativa Hibridación Interespecífica In Vitro Experimentation Induced Polyploidy Vegetative Propagation Interspecific Hybridization Glandularia The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program. Instituto de Floricultura Fil: Gonzalez Roca, Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina Fil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina Fil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina Fil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina Fil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología Algal y Vegetal; Argentina Fil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina 2018-07-26T12:55:08Z 2018-07-26T12:55:08Z 2015-03-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2883 https://www.sciencedirect.com/science/article/pii/S0304423814007055 0304-4238 https://doi.org/10.1016/j.scienta.2014.12.032 eng info:eu-repo/semantics/restrictedAccess application/pdf Scientia Horticulturae 184 : 46-54 (March 2015) |
| spellingShingle | Verbenaceae Experimentación in Vitro Poliploidia Inducida Propagación Vegetativa Hibridación Interespecífica In Vitro Experimentation Induced Polyploidy Vegetative Propagation Interspecific Hybridization Glandularia Gonzalez Roca, Lucia Iannicelli, Jesica Coviella, Maria Andrea Bugallo, Veronica Lucia Bologna, Paula Pitta Alvarez, Sandra Irene Escandon, Alejandro Salvio A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) |
| title | A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) |
| title_full | A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) |
| title_fullStr | A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) |
| title_full_unstemmed | A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) |
| title_short | A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata) |
| title_sort | protocol for the in vitro propagation and polyploidization of an interspecific hybrid of glandularia g peruviana g scrobiculata |
| topic | Verbenaceae Experimentación in Vitro Poliploidia Inducida Propagación Vegetativa Hibridación Interespecífica In Vitro Experimentation Induced Polyploidy Vegetative Propagation Interspecific Hybridization Glandularia |
| url | http://hdl.handle.net/20.500.12123/2883 https://www.sciencedirect.com/science/article/pii/S0304423814007055 https://doi.org/10.1016/j.scienta.2014.12.032 |
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