On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling
An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely,...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2824 https://doi.org/10.1016/j.microc.2014.12.010 |
| _version_ | 1855483127086972928 |
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| author | Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora |
| author_browse | Befani, Romina Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Smichowski, Patricia Nora Soro, Enrique Mariano |
| author_facet | Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora |
| author_sort | Farías, Silvia Sara |
| collection | INTA Digital |
| description | An investigation was carried out to explore further the analytical capabilities of the coupling high performance
liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the
reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V),
monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different
cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild
conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation
included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three
species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness
(bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation
data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to
1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS
was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in
the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of
the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. |
| format | Artículo |
| id | INTA2824 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28242018-07-19T13:37:59Z On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora Arroz Oryza Sativa Arsénico HPLC Toxicidad Toxicity Arsenic Rice HPLC–HG–AFS Mild Extraction Speciation Analysis of As Argentina An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness (bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to 1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. EEA Concepción del Uruguay Fil: Farías, Silvia Sara. Comisión Nacional de Energía Atómica; Argentina Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Quintero, Cesar Eugenio. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina Fil: Befani, Romina. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; Argentina Fil: Soro, Enrique Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Smichowski, Patricia Nora. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-07-19T13:22:37Z 2018-07-19T13:22:37Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article http://hdl.handle.net/20.500.12123/2824 0026-265X https://doi.org/10.1016/j.microc.2014.12.010 eng info:eu-repo/semantics/restrictedAccess application/pdf Microchemical journal 120 : 34–39. (2015) |
| spellingShingle | Arroz Oryza Sativa Arsénico HPLC Toxicidad Toxicity Arsenic Rice HPLC–HG–AFS Mild Extraction Speciation Analysis of As Argentina Farías, Silvia Sara Londonio, Juan Agustín Quintero, César Eugenio Befani, Romina Soro, Enrique Mariano Smichowski, Patricia Nora On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title | On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_full | On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_fullStr | On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_full_unstemmed | On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_short | On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling |
| title_sort | on line speciation and quantification of four arsenical species in rice samples collected in argentina using a hplc hg afs coupling |
| topic | Arroz Oryza Sativa Arsénico HPLC Toxicidad Toxicity Arsenic Rice HPLC–HG–AFS Mild Extraction Speciation Analysis of As Argentina |
| url | http://hdl.handle.net/20.500.12123/2824 https://doi.org/10.1016/j.microc.2014.12.010 |
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