On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC–HG–AFS coupling
An investigation was carried out to explore further the analytical capabilities of the coupling high performance liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the reliable determination of the four toxicologically relevant arsenical species namely,...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2824 https://doi.org/10.1016/j.microc.2014.12.010 |
| Sumario: | An investigation was carried out to explore further the analytical capabilities of the coupling high performance
liquid chromatography (HPLC)–hydride generation (HG)–atomic fluorescence spectrometry (AFS) for the
reliable determination of the four toxicologically relevant arsenical species namely, As(III), As(V),
monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in rice. Fifty nine samples of rice of five different
cultivars were collected in province of Entre Rios (Argentina). Samples were dried at 103 ± 2 °C and mild
conditions were adopted for species extraction (0.28 mol L−1 HNO3 at 95± 3 °C for 90 min). Method validation
included, evaluation of linearity; limits of detection, 0.020 μg g−1 for As(III) and 0.025 μg g−1 for the other three
species; precision (intermediate precision), 4% for As(III) and DMA, and 6% for the other two species; trueness
(bias), 9% for As(III) and As(V) and 11% for the other two species, and uncertainty (obtained from validation
data) varying from 9.5% for As(III) to 19% for As(V). Total arsenic concentrations ranged from 0.08 to
1.39 mg kg−1. The mean concentrations of sum of the As species extracted and determined by HPLC–HG–AFS
was approximately 99.7% of certified value for total As in NIST SRM “Rice flour”. Levels of the four species in
the analyzed samples were in the order DMA N As(III) N As(V) N MMA. Inorganic As (iAs) accounted for 28% of
the As detected. DMA exhibited the highest levels, representing a 72% of total grain As on average. |
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