Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina
The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniae strains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. Based on the serological tests, forty-seven field strains of Actinobacillus pleuropne...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-articulo-characterization-actinobacillus-pleuropneumoniae-field-strains-S0325754117300901 http://hdl.handle.net/20.500.12123/2752 https://doi.org/10.1016/j.ram.2017.04.010 |
| _version_ | 1855483113993404416 |
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| author | To, Ho Teshima, Kaho Nagai, Shinya Zielinski, Gustavo Carlos Koyama, Tomohiro Lee, Jina Bessone, Fernando Aníbal Nagano, Tetsuji Oshima, Atsushi Tsutsumi, Nobuyuki |
| author_browse | Bessone, Fernando Aníbal Koyama, Tomohiro Lee, Jina Nagai, Shinya Nagano, Tetsuji Oshima, Atsushi Teshima, Kaho To, Ho Tsutsumi, Nobuyuki Zielinski, Gustavo Carlos |
| author_facet | To, Ho Teshima, Kaho Nagai, Shinya Zielinski, Gustavo Carlos Koyama, Tomohiro Lee, Jina Bessone, Fernando Aníbal Nagano, Tetsuji Oshima, Atsushi Tsutsumi, Nobuyuki |
| author_sort | To, Ho |
| collection | INTA Digital |
| description | The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniae strains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. Based on the serological tests, forty-seven field strains of Actinobacillus pleuropneumoniae isolated from lungs with pleuropneumonia lesions in Japan and Argentina were found to be serovars belonging to the 3-6-8-15 cross-reacting group. By using a capsule loci-based PCR, twenty-nine (96.7%) and one (3.3%) from Japan were identified as serovars 15 and 8, respectively, whereas seventeen (100%) from Argentina were identified as serovar 8. The findings suggested that serovars 8 and 15 were prevalent within the 3-6-8-15 cross-reacting group, in Argentina and Japan, respectively. Phenotypic analyses revealed that the protein patterns observed on SDS–PAGE and the lipopolysaccharide antigen detected by immunoblotting of the reference and field strains of serovars 8 and 15 were similar to each other. Genetic (16S rDNA, apxIIA, apxIIIA, cps, cpx genes, apx and omlA patterns) analyses revealed that the apxIIA and apxIIIA genes of the field strains of serovars 8 and 15 were similar to those of the reference strains of serovars 3, 4, 6, 8 and 15. The results obtained in the present study may be useful for the development of more effective vaccines against disease caused by A. pleuropneumoniae by including the homologous antigens to the most prevalent serovars in specific geographical areas. |
| format | Artículo |
| id | INTA2752 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27522018-07-10T15:41:14Z Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina To, Ho Teshima, Kaho Nagai, Shinya Zielinski, Gustavo Carlos Koyama, Tomohiro Lee, Jina Bessone, Fernando Aníbal Nagano, Tetsuji Oshima, Atsushi Tsutsumi, Nobuyuki Actinobacillus pleuropneumoniae Cerdo Enfermedades de los Animales Fenotipos Genética Swine Animal Diseases Phenotypes Genetics Japón Argentina The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniae strains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. Based on the serological tests, forty-seven field strains of Actinobacillus pleuropneumoniae isolated from lungs with pleuropneumonia lesions in Japan and Argentina were found to be serovars belonging to the 3-6-8-15 cross-reacting group. By using a capsule loci-based PCR, twenty-nine (96.7%) and one (3.3%) from Japan were identified as serovars 15 and 8, respectively, whereas seventeen (100%) from Argentina were identified as serovar 8. The findings suggested that serovars 8 and 15 were prevalent within the 3-6-8-15 cross-reacting group, in Argentina and Japan, respectively. Phenotypic analyses revealed that the protein patterns observed on SDS–PAGE and the lipopolysaccharide antigen detected by immunoblotting of the reference and field strains of serovars 8 and 15 were similar to each other. Genetic (16S rDNA, apxIIA, apxIIIA, cps, cpx genes, apx and omlA patterns) analyses revealed that the apxIIA and apxIIIA genes of the field strains of serovars 8 and 15 were similar to those of the reference strains of serovars 3, 4, 6, 8 and 15. The results obtained in the present study may be useful for the development of more effective vaccines against disease caused by A. pleuropneumoniae by including the homologous antigens to the most prevalent serovars in specific geographical areas. Los objetivos del presente estudio fueron determinar el serovar de una colección de cepas de Actinobacillus pleuropneumoniae pertenecientes al grupo 3, 6, 8, 15 de reacciones cruzadas y analizar sus propiedades fenotípicas y genéticas. En base a técnicas serológicas se determinó que cuarenta y siete cepas de A. pleuropneumoniae aisladas a partir de pulmones con lesiones de pleuroneumonía en Japón y Argentina pertenecen al grupo 3, 6, 8, 15. Mediante el uso de PCR basado en locus capsulares, veintinueve (96.7%) y una (3.3%) de los aislados japoneses fueron identificados como serovar 15 y 8 respectivamente, mientras que diecisiete (100%) de los aislados argentinos resultaron pertenecer al serotipo 8. Este hallazgo sugirió que los serovares 8 y 15 fueron los prevalentes dentro del grupo 3, 6, 8, 15 en Japón y Argentina, respectivamente. El análisis fenotípico reveló que los perfiles proteicos determinados por SDS-PAGE, y de antígenos lipopolisacáridos estudiados por inmunoblot, de las cepas de referencia y de campo de los serovares 8 y 15 fueron similares entre sí. El análisis genético (16S rDNA, apxIIA, apxIIA, cps, genes cpx, apx y los perfiles omlA) reveló que los genes apxIIA y apxIIIA de las cepas de campo de los serovares 8 y 15 fueron similares a sus homólogos de las cepas de referencia de los serovares 3, 4, 6, 8 y 15. Los resultados obtenidos en el presente estudio pueden ser útiles para el desarrollo de vacunas más efectivas contra la enfermedad causada por A. pleuropneumoniae, al posibilitar incluir antígenos homólogos a los serovares prevalentes en las áreas geográficas de interés. EEA Marcos Juárez Fil: To, Ho. Nippon Institute for Biological Science; Japón Fil: Teshima, Kaho. Nippon Institute for Biological Science; Japón Fil: Nagai, Shinya. Nippon Institute for Biological Science; Japón Fil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Koyama, Tomohiro. Nippon Institute for Biological Science; Japón Fil: Lee, Jina. Nippon Institute for Biological Science; Japón Fil: Bessone, Fernando Aníbal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Nagano, Tetsuji. Nippon Institute for Biological Science; Japón Fil: Oshima, Atsushi. Nippon Institute for Biological Science; Japón Fil: Tsutsumi, Nobuyuki. Nippon Institute for Biological Science; Japón 2018-07-10T15:39:19Z 2018-07-10T15:39:19Z 2018-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-articulo-characterization-actinobacillus-pleuropneumoniae-field-strains-S0325754117300901 http://hdl.handle.net/20.500.12123/2752 0325-7541 1851-7617 https://doi.org/10.1016/j.ram.2017.04.010 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Revista Argentina de Microbiología 50 (1) : 12-22. (January-March 2018) |
| spellingShingle | Actinobacillus pleuropneumoniae Cerdo Enfermedades de los Animales Fenotipos Genética Swine Animal Diseases Phenotypes Genetics Japón Argentina To, Ho Teshima, Kaho Nagai, Shinya Zielinski, Gustavo Carlos Koyama, Tomohiro Lee, Jina Bessone, Fernando Aníbal Nagano, Tetsuji Oshima, Atsushi Tsutsumi, Nobuyuki Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina |
| title | Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina |
| title_full | Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina |
| title_fullStr | Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina |
| title_full_unstemmed | Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina |
| title_short | Characterization of Actinobacillus pleuropneumoniae field strains antigenically related to the 3-6-8-15 group from diseased pigs in Japan and Argentina = Caracterización de cepas de Actinobacillus pleuropneumoniae antigénicamente relacionados al grupo 3-6-8-15 obtenidos de cerdos infectados naturalmente en Japón y Argentina |
| title_sort | characterization of actinobacillus pleuropneumoniae field strains antigenically related to the 3 6 8 15 group from diseased pigs in japan and argentina caracterizacion de cepas de actinobacillus pleuropneumoniae antigenicamente relacionados al grupo 3 6 8 15 obtenidos de cerdos infectados naturalmente en japon y argentina |
| topic | Actinobacillus pleuropneumoniae Cerdo Enfermedades de los Animales Fenotipos Genética Swine Animal Diseases Phenotypes Genetics Japón Argentina |
| url | http://www.elsevier.es/en-revista-revista-argentina-microbiologia-372-articulo-characterization-actinobacillus-pleuropneumoniae-field-strains-S0325754117300901 http://hdl.handle.net/20.500.12123/2752 https://doi.org/10.1016/j.ram.2017.04.010 |
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