The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing
Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/pii/S0168170213004474 http://hdl.handle.net/20.500.12123/2564 https://doi.org/10.1016/j.virusres.2013.12.018 |
| _version_ | 1855034985877078016 |
|---|---|
| author | Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Casse, Maria Florencia Bonacic Kresic, Iván Hopp, Horacio Esteban Ziegler-Graff, Véronique Distefano, Ana Julia |
| author_browse | Agrofoglio, Yamila Carla Bonacic Kresic, Iván Casse, Maria Florencia Delfosse, Veronica Cecilia Distefano, Ana Julia Hopp, Horacio Esteban Ziegler-Graff, Véronique |
| author_facet | Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Casse, Maria Florencia Bonacic Kresic, Iván Hopp, Horacio Esteban Ziegler-Graff, Véronique Distefano, Ana Julia |
| author_sort | Delfosse, Veronica Cecilia |
| collection | INTA Digital |
| description | Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2564 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA25642018-07-05T13:32:36Z The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Casse, Maria Florencia Bonacic Kresic, Iván Hopp, Horacio Esteban Ziegler-Graff, Véronique Distefano, Ana Julia Virus de las Plantas Algodón Proteínas ARN Plant Viruses Cotton Proteins RNA Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. Instituto de Biotecnología Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Casse, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina Fil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Ziegler-Graff, Véronique. Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS conventionné avec l’Université de Strasbourg; Francia Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2018-06-07T12:19:12Z 2018-06-07T12:19:12Z 2014-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0168170213004474 http://hdl.handle.net/20.500.12123/2564 0168-1702 https://doi.org/10.1016/j.virusres.2013.12.018 eng info:eu-repo/semantics/restrictedAccess application/pdf Virus research 180 : 70-75. (February 2014) |
| spellingShingle | Virus de las Plantas Algodón Proteínas ARN Plant Viruses Cotton Proteins RNA Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Casse, Maria Florencia Bonacic Kresic, Iván Hopp, Horacio Esteban Ziegler-Graff, Véronique Distefano, Ana Julia The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title | The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_full | The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_fullStr | The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_full_unstemmed | The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_short | The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_sort | p0 protein encoded by cotton leafroll dwarf virus clrdv inhibits local but not systemic rna silencing |
| topic | Virus de las Plantas Algodón Proteínas ARN Plant Viruses Cotton Proteins RNA |
| url | https://www.sciencedirect.com/science/article/pii/S0168170213004474 http://hdl.handle.net/20.500.12123/2564 https://doi.org/10.1016/j.virusres.2013.12.018 |
| work_keys_str_mv | AT delfosseveronicacecilia thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT agrofoglioyamilacarla thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT cassemariaflorencia thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT bonacickresicivan thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT hopphoracioesteban thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT zieglergraffveronique thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT distefanoanajulia thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT delfosseveronicacecilia p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT agrofoglioyamilacarla p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT cassemariaflorencia p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT bonacickresicivan p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT hopphoracioesteban p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT zieglergraffveronique p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT distefanoanajulia p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing |