Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies
Bovine gammaherpesvirus 4 (BoGHV-4) has been frequently associated with postpartum uterine infections in cattle; however, its pathogenesis and interaction with bacterial components remain poorly understood. This study investigated the effects of BoGHV-4 infection and Escherichia coli lipopolysacchar...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/25011 https://www.sciencedirect.com/science/article/pii/S2950194625003747 https://doi.org/10.1016/j.microb.2025.100606 |
| _version_ | 1855039045801869312 |
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| author | Romeo, Florencia Delgado, Santiago Pereyra, Susana Beatriz Lopez, Sofia Pérez, Sandra Verna, Andrea Elizabeth |
| author_browse | Delgado, Santiago Lopez, Sofia Pereyra, Susana Beatriz Pérez, Sandra Romeo, Florencia Verna, Andrea Elizabeth |
| author_facet | Romeo, Florencia Delgado, Santiago Pereyra, Susana Beatriz Lopez, Sofia Pérez, Sandra Verna, Andrea Elizabeth |
| author_sort | Romeo, Florencia |
| collection | INTA Digital |
| description | Bovine gammaherpesvirus 4 (BoGHV-4) has been frequently associated with postpartum uterine infections in cattle; however, its pathogenesis and interaction with bacterial components remain poorly understood. This study investigated the effects of BoGHV-4 infection and Escherichia coli lipopolysaccharide (LPS) on Toll-like receptor 4 (TLR4) activation and downstream immune modulation in primary bovine endometrial cells (BECs). The expression and secretion of TLR4, CXCL8, interferon-γ (IFN-γ), and tumour necrosis factor-α (TNF-α) were evaluated over time. LPS markedly upregulated TLR4 expression, whereas BoGHV-4 alone led to late-stage
downregulation. Co-stimulation with BoGHV-4 and LPS initially enhanced TLR4 expression but resulted in suppression at later time points. CXCL8 and IFN-γ levels increased significantly, particularly under BoGHV4 +LPS conditions, suggesting synergistic amplification of inflammatory signalling. Conversely, TNF-α remained undetectable, implying tight post-transcriptional control. These findings highlight the intricate virus–bacterium interplay in the bovine endometrium and provide mechanistic insight into BoGHV-4-associated uterine pathology. |
| format | info:ar-repo/semantics/artículo |
| id | INTA25011 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA250112026-01-20T18:01:16Z Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies Romeo, Florencia Delgado, Santiago Pereyra, Susana Beatriz Lopez, Sofia Pérez, Sandra Verna, Andrea Elizabeth Bovinae Ganado Bovino Período Perinatal Enfermedades de los Animales Enfermedad Uterina Cattle Perinatal Period Animal Diseases Uterine Diseases Bovine gammaherpesvirus 4 (BoGHV-4) Escherichia coli lipopolysaccharide (LPS) Postpartum Uterine Infections Bovine gammaherpesvirus 4 (BoGHV-4) has been frequently associated with postpartum uterine infections in cattle; however, its pathogenesis and interaction with bacterial components remain poorly understood. This study investigated the effects of BoGHV-4 infection and Escherichia coli lipopolysaccharide (LPS) on Toll-like receptor 4 (TLR4) activation and downstream immune modulation in primary bovine endometrial cells (BECs). The expression and secretion of TLR4, CXCL8, interferon-γ (IFN-γ), and tumour necrosis factor-α (TNF-α) were evaluated over time. LPS markedly upregulated TLR4 expression, whereas BoGHV-4 alone led to late-stage downregulation. Co-stimulation with BoGHV-4 and LPS initially enhanced TLR4 expression but resulted in suppression at later time points. CXCL8 and IFN-γ levels increased significantly, particularly under BoGHV4 +LPS conditions, suggesting synergistic amplification of inflammatory signalling. Conversely, TNF-α remained undetectable, implying tight post-transcriptional control. These findings highlight the intricate virus–bacterium interplay in the bovine endometrium and provide mechanistic insight into BoGHV-4-associated uterine pathology. EEA Balcarce Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Delgado, Santiago. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Pereyra, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Lopez, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Lopez, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias; Argentina Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina 2026-01-20T17:58:06Z 2026-01-20T17:58:06Z 2025-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/25011 https://www.sciencedirect.com/science/article/pii/S2950194625003747 2950-1946 https://doi.org/10.1016/j.microb.2025.100606 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier The Microbe 9 : 100606. (December 2025) |
| spellingShingle | Bovinae Ganado Bovino Período Perinatal Enfermedades de los Animales Enfermedad Uterina Cattle Perinatal Period Animal Diseases Uterine Diseases Bovine gammaherpesvirus 4 (BoGHV-4) Escherichia coli lipopolysaccharide (LPS) Postpartum Uterine Infections Romeo, Florencia Delgado, Santiago Pereyra, Susana Beatriz Lopez, Sofia Pérez, Sandra Verna, Andrea Elizabeth Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies |
| title | Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies |
| title_full | Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies |
| title_fullStr | Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies |
| title_full_unstemmed | Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies |
| title_short | Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies |
| title_sort | effects of boghv 4 and lps on key inflammatory molecules insights from primary culture studies |
| topic | Bovinae Ganado Bovino Período Perinatal Enfermedades de los Animales Enfermedad Uterina Cattle Perinatal Period Animal Diseases Uterine Diseases Bovine gammaherpesvirus 4 (BoGHV-4) Escherichia coli lipopolysaccharide (LPS) Postpartum Uterine Infections |
| url | http://hdl.handle.net/20.500.12123/25011 https://www.sciencedirect.com/science/article/pii/S2950194625003747 https://doi.org/10.1016/j.microb.2025.100606 |
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