Effects of BoGHV-4 and LPS on key inflammatory molecules: Insights from primary culture studies
Bovine gammaherpesvirus 4 (BoGHV-4) has been frequently associated with postpartum uterine infections in cattle; however, its pathogenesis and interaction with bacterial components remain poorly understood. This study investigated the effects of BoGHV-4 infection and Escherichia coli lipopolysacchar...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/25011 https://www.sciencedirect.com/science/article/pii/S2950194625003747 https://doi.org/10.1016/j.microb.2025.100606 |
| Sumario: | Bovine gammaherpesvirus 4 (BoGHV-4) has been frequently associated with postpartum uterine infections in cattle; however, its pathogenesis and interaction with bacterial components remain poorly understood. This study investigated the effects of BoGHV-4 infection and Escherichia coli lipopolysaccharide (LPS) on Toll-like receptor 4 (TLR4) activation and downstream immune modulation in primary bovine endometrial cells (BECs). The expression and secretion of TLR4, CXCL8, interferon-γ (IFN-γ), and tumour necrosis factor-α (TNF-α) were evaluated over time. LPS markedly upregulated TLR4 expression, whereas BoGHV-4 alone led to late-stage
downregulation. Co-stimulation with BoGHV-4 and LPS initially enhanced TLR4 expression but resulted in suppression at later time points. CXCL8 and IFN-γ levels increased significantly, particularly under BoGHV4 +LPS conditions, suggesting synergistic amplification of inflammatory signalling. Conversely, TNF-α remained undetectable, implying tight post-transcriptional control. These findings highlight the intricate virus–bacterium interplay in the bovine endometrium and provide mechanistic insight into BoGHV-4-associated uterine pathology. |
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