Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model
Campylobacter spp., and especially C. jejuni, are leading causes of bacterial-derived foodborne illness in humans. Poultry are known reservoirs, and in many instances, Campylobacter are thought to be commensal organisms harmlessly residing in the gastrointestinal tract of poultry; therefore, hum...
| Autores principales: | , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2026
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24987 https://doi.org/10.1016/j.psj.2025.106019 |
| _version_ | 1855039041457618944 |
|---|---|
| author | Swaggerty, Christina L. Velilla, Alejandra Vanesa Genovese, Kenneth J. Allen Byrd, J. Kogut, Michael H. |
| author_browse | Allen Byrd, J. Genovese, Kenneth J. Kogut, Michael H. Swaggerty, Christina L. Velilla, Alejandra Vanesa |
| author_facet | Swaggerty, Christina L. Velilla, Alejandra Vanesa Genovese, Kenneth J. Allen Byrd, J. Kogut, Michael H. |
| author_sort | Swaggerty, Christina L. |
| collection | INTA Digital |
| description | Campylobacter spp., and especially C. jejuni, are leading causes of bacterial-derived foodborne illness in humans.
Poultry are known reservoirs, and in many instances, Campylobacter are thought to be commensal organisms
harmlessly residing in the gastrointestinal tract of poultry; therefore, human illness could result from mishan dling or consuming raw or undercooked chicken products contaminated with Campylobacter. The objective of this
study was to prime the chickens with a low dose of C. jejuni at a young age followed by a higher dose later and
monitor changes to key inflammatory and regulatory cytokines and chemokines. Broiler chicks (2-days-of-age)
were administered 1 × 103 colony forming units (cfu)/mL C. jejuni, (0.5 mL, orally) followed by a second dosage
of 1 × 107 cfu/mL (1 mL, orally) at 28-d-of-age. At 35-d-of-age, the chickens were necropsied and the cecal tonsil
was collected. The study was conducted twice. Cytokine (IL1β, IL6, IL10, IFNγ, TGFβ4) and chemokine (CXCL8)
mRNA expression was measured in the cecal tonsil. Expression of IL1β, IL10, TGFβ4, and CXCL8 mRNA were
significantly higher (2.6 - 5.8-fold; P ≤ 0.04) in the cecal tonsils of the chickens subjected to the two doses of
C. jejuni. The C. jejuni challenges had no effect on the mRNA expression levels of IL6 and IFNγ (P > 0.05). These
data indicate repeated exposure to C. jejuni stimulates a diverse and robust cytokine response in important
lymphoid tissue indicating the host recognizes the C. jejuni as pathogenic and mounts a response yet a persistent
infection is still established. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24987 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA249872026-01-14T22:18:39Z Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model Swaggerty, Christina L. Velilla, Alejandra Vanesa Genovese, Kenneth J. Allen Byrd, J. Kogut, Michael H. Enfermedad Bacteriana Enfermedad Transmitida por Alimentos Campylobacter jejuni Reservorio de Enfermedad Ave de Corral Bacterial Diseases Foodborne Diseases Disease Reservoirs Poultry Campylobacter spp., and especially C. jejuni, are leading causes of bacterial-derived foodborne illness in humans. Poultry are known reservoirs, and in many instances, Campylobacter are thought to be commensal organisms harmlessly residing in the gastrointestinal tract of poultry; therefore, human illness could result from mishan dling or consuming raw or undercooked chicken products contaminated with Campylobacter. The objective of this study was to prime the chickens with a low dose of C. jejuni at a young age followed by a higher dose later and monitor changes to key inflammatory and regulatory cytokines and chemokines. Broiler chicks (2-days-of-age) were administered 1 × 103 colony forming units (cfu)/mL C. jejuni, (0.5 mL, orally) followed by a second dosage of 1 × 107 cfu/mL (1 mL, orally) at 28-d-of-age. At 35-d-of-age, the chickens were necropsied and the cecal tonsil was collected. The study was conducted twice. Cytokine (IL1β, IL6, IL10, IFNγ, TGFβ4) and chemokine (CXCL8) mRNA expression was measured in the cecal tonsil. Expression of IL1β, IL10, TGFβ4, and CXCL8 mRNA were significantly higher (2.6 - 5.8-fold; P ≤ 0.04) in the cecal tonsils of the chickens subjected to the two doses of C. jejuni. The C. jejuni challenges had no effect on the mRNA expression levels of IL6 and IFNγ (P > 0.05). These data indicate repeated exposure to C. jejuni stimulates a diverse and robust cytokine response in important lymphoid tissue indicating the host recognizes the C. jejuni as pathogenic and mounts a response yet a persistent infection is still established. EEA Balcarce Fil: Swaggerty, Christina L.Department of Agriculture. Southern Plains Agricultural Research Center; Estados Unidos Fil: Velilla, Alejandra Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina Fil: Genovese, Kenneth J. Department of Agriculture. Southern Plains Agricultural Research Center; Estados Unidos Fil:Allen Byrd, J. Department of Agriculture. Southern Plains Agricultural Research Center; Estados Unidos Fil: Kogut, Michael H. Department of Agriculture. Southern Plains Agricultural Research Center; Estados Unidos 2026-01-14T22:00:04Z 2026-01-14T22:00:04Z 2025-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24987 1525-3171 https://doi.org/10.1016/j.psj.2025.106019 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Poultry Science 104 (2025) 106019 |
| spellingShingle | Enfermedad Bacteriana Enfermedad Transmitida por Alimentos Campylobacter jejuni Reservorio de Enfermedad Ave de Corral Bacterial Diseases Foodborne Diseases Disease Reservoirs Poultry Swaggerty, Christina L. Velilla, Alejandra Vanesa Genovese, Kenneth J. Allen Byrd, J. Kogut, Michael H. Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| title | Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| title_full | Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| title_fullStr | Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| title_full_unstemmed | Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| title_short | Campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| title_sort | campylobacter jejuni stimulates a robust cytokine response in the cecal tonsil following a repeated challenge infection model |
| topic | Enfermedad Bacteriana Enfermedad Transmitida por Alimentos Campylobacter jejuni Reservorio de Enfermedad Ave de Corral Bacterial Diseases Foodborne Diseases Disease Reservoirs Poultry |
| url | http://hdl.handle.net/20.500.12123/24987 https://doi.org/10.1016/j.psj.2025.106019 |
| work_keys_str_mv | AT swaggertychristinal campylobacterjejunistimulatesarobustcytokineresponseinthececaltonsilfollowingarepeatedchallengeinfectionmodel AT velillaalejandravanesa campylobacterjejunistimulatesarobustcytokineresponseinthececaltonsilfollowingarepeatedchallengeinfectionmodel AT genovesekennethj campylobacterjejunistimulatesarobustcytokineresponseinthececaltonsilfollowingarepeatedchallengeinfectionmodel AT allenbyrdj campylobacterjejunistimulatesarobustcytokineresponseinthececaltonsilfollowingarepeatedchallengeinfectionmodel AT kogutmichaelh campylobacterjejunistimulatesarobustcytokineresponseinthececaltonsilfollowingarepeatedchallengeinfectionmodel |