Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV)...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24807 https://www.mdpi.com/1999-4915/17/7/995 https://doi.org/10.3390/v17070995 |
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| author | Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_browse | Bejerman, Nicolas Esteban Laguna, Irma Graciela Maugeri Suarez, Mariel Rodriguez Pardina, Patricia Rodriguez, Marianela |
| author_facet | Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_sort | Maugeri Suarez, Mariel |
| collection | INTA Digital |
| description | Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at −70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24807 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA248072025-12-30T11:44:09Z Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia Soja Virus de las Plantas Virus del Mosaico Variación Genética Argentina Soybeans Plant Viruses Glycine max Mosaic Viruses Genetic Variation Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at −70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars. Instituto de Patología Vegetal Fil: Maugeri Suarez, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2025-12-30T11:41:10Z 2025-12-30T11:41:10Z 2025-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24807 https://www.mdpi.com/1999-4915/17/7/995 1999-4915 https://doi.org/10.3390/v17070995 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 17 (7) : 995. (July 2025) |
| spellingShingle | Soja Virus de las Plantas Virus del Mosaico Variación Genética Argentina Soybeans Plant Viruses Glycine max Mosaic Viruses Genetic Variation Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops |
| title | Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops |
| title_full | Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops |
| title_fullStr | Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops |
| title_full_unstemmed | Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops |
| title_short | Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops |
| title_sort | biological molecular and physiological characterization of four soybean mosaic virus isolates present in argentine soybean crops |
| topic | Soja Virus de las Plantas Virus del Mosaico Variación Genética Argentina Soybeans Plant Viruses Glycine max Mosaic Viruses Genetic Variation |
| url | http://hdl.handle.net/20.500.12123/24807 https://www.mdpi.com/1999-4915/17/7/995 https://doi.org/10.3390/v17070995 |
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