Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-s...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs10535-018-0794-3 http://hdl.handle.net/20.500.12123/2422 https://doi.org/10.1007/s10535-018-0794-3 |
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| author | Ruggieri, Germán Triassi, Agustina Alvarez, Clarisa E. Gola, Aldana Wiggenhauser, Julieta Budde, Claudio Olaf Lara, María Valeria Müller, Gabriela Leticia |
| author_browse | Alvarez, Clarisa E. Budde, Claudio Olaf Gola, Aldana Lara, María Valeria Müller, Gabriela Leticia Ruggieri, Germán Triassi, Agustina Wiggenhauser, Julieta |
| author_facet | Ruggieri, Germán Triassi, Agustina Alvarez, Clarisa E. Gola, Aldana Wiggenhauser, Julieta Budde, Claudio Olaf Lara, María Valeria Müller, Gabriela Leticia |
| author_sort | Ruggieri, Germán |
| collection | INTA Digital |
| description | Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions. |
| format | Artículo |
| id | INTA2422 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24222018-08-21T15:51:04Z Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage Ruggieri, Germán Triassi, Agustina Alvarez, Clarisa E. Gola, Aldana Wiggenhauser, Julieta Budde, Claudio Olaf Lara, María Valeria Müller, Gabriela Leticia Tomate Solanum lycopersicum Aminoácidos Ritmo Circadiano Poligalacturonasa ARN Almacenamiento en frío Proteínas Tomatoes Amino Acids Circadian Rhythm Polygalacturonase RNA Cold Storage Proteins Lycopersicon esculentum Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions. EEA San Pedro Fil: Ruggieri, German. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Álvarez, Clarisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Gola, Aldana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Wiggenhauser, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Lara, Maria Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Müller, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina 2018-05-17T17:34:15Z 2018-05-17T17:34:15Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://link.springer.com/article/10.1007%2Fs10535-018-0794-3 http://hdl.handle.net/20.500.12123/2422 1573-8264 0006-3134 https://doi.org/10.1007/s10535-018-0794-3 eng info:eu-repo/semantics/embargoedAccess application/pdf Biologia Plantarum 62 (3) : 501-510 (2018) |
| spellingShingle | Tomate Solanum lycopersicum Aminoácidos Ritmo Circadiano Poligalacturonasa ARN Almacenamiento en frío Proteínas Tomatoes Amino Acids Circadian Rhythm Polygalacturonase RNA Cold Storage Proteins Lycopersicon esculentum Ruggieri, Germán Triassi, Agustina Alvarez, Clarisa E. Gola, Aldana Wiggenhauser, Julieta Budde, Claudio Olaf Lara, María Valeria Müller, Gabriela Leticia Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage |
| title | Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage |
| title_full | Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage |
| title_fullStr | Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage |
| title_full_unstemmed | Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage |
| title_short | Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage |
| title_sort | overexpression of glycine rich rna binding protein in tomato renders fruits with higher protein content after cold storage |
| topic | Tomate Solanum lycopersicum Aminoácidos Ritmo Circadiano Poligalacturonasa ARN Almacenamiento en frío Proteínas Tomatoes Amino Acids Circadian Rhythm Polygalacturonase RNA Cold Storage Proteins Lycopersicon esculentum |
| url | https://link.springer.com/article/10.1007%2Fs10535-018-0794-3 http://hdl.handle.net/20.500.12123/2422 https://doi.org/10.1007/s10535-018-0794-3 |
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