First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes
Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. Accord...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://link.springer.com/article/10.1007/s00705-014-2146-8 http://hdl.handle.net/20.500.12123/2393 https://doi.org/10.1007/s00705-014-2146-8 |
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| author | Maidana, Silvina Soledad Konrad, Jose Luis Craig, María Isabel Zabal, Osvaldo Alfredo Mauroy, Axel Thiry, Etienne Crudeli, Gustavo Angel Romera, Sonia Alejandra |
| author_browse | Craig, María Isabel Crudeli, Gustavo Angel Konrad, Jose Luis Maidana, Silvina Soledad Mauroy, Axel Romera, Sonia Alejandra Thiry, Etienne Zabal, Osvaldo Alfredo |
| author_facet | Maidana, Silvina Soledad Konrad, Jose Luis Craig, María Isabel Zabal, Osvaldo Alfredo Mauroy, Axel Thiry, Etienne Crudeli, Gustavo Angel Romera, Sonia Alejandra |
| author_sort | Maidana, Silvina Soledad |
| collection | INTA Digital |
| description | Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2393 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA23932018-05-14T15:20:44Z First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes Maidana, Silvina Soledad Konrad, Jose Luis Craig, María Isabel Zabal, Osvaldo Alfredo Mauroy, Axel Thiry, Etienne Crudeli, Gustavo Angel Romera, Sonia Alejandra Búfalo de Agua Enfermedades de los Animales Herpesviridae Técnicas de Aislamiento Genética Water Buffaloes Animal Diseases Isolation Techniques Genetics Herpesvirus Argentina Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. Instituto de Virología Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina Fil: Konrad, Jose Luis. Universidad Nacional del Nordeste. Facultad de Veterinaria. Cátedra de Teriogenologia; Argentina Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Crudeli, Gustavo Angel. Universidad Nacional del Nordeste. Facultad de Veterinaria. Cátedra de Teriogenologia; Argentina Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina 2018-05-14T15:19:15Z 2018-05-14T15:19:15Z 2014-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00705-014-2146-8 http://hdl.handle.net/20.500.12123/2393 0304-8608 1432-8798 https://doi.org/10.1007/s00705-014-2146-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Archives of Virology 159 (11) : 2917–2923. (November 2014) |
| spellingShingle | Búfalo de Agua Enfermedades de los Animales Herpesviridae Técnicas de Aislamiento Genética Water Buffaloes Animal Diseases Isolation Techniques Genetics Herpesvirus Argentina Maidana, Silvina Soledad Konrad, Jose Luis Craig, María Isabel Zabal, Osvaldo Alfredo Mauroy, Axel Thiry, Etienne Crudeli, Gustavo Angel Romera, Sonia Alejandra First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes |
| title | First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes |
| title_full | First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes |
| title_fullStr | First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes |
| title_full_unstemmed | First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes |
| title_short | First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes |
| title_sort | first report of isolation and molecular characterization of bubaline herpesvirus 1 buhv1 from argentinean water buffaloes |
| topic | Búfalo de Agua Enfermedades de los Animales Herpesviridae Técnicas de Aislamiento Genética Water Buffaloes Animal Diseases Isolation Techniques Genetics Herpesvirus Argentina |
| url | https://link.springer.com/article/10.1007/s00705-014-2146-8 http://hdl.handle.net/20.500.12123/2393 https://doi.org/10.1007/s00705-014-2146-8 |
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