Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy

Purple carrot is an important source of anthocyanins as a natural food dye, yet research advancements in selecting purple cultivars with improved anthocyanin extractable yield and stability remain limited. This study used a large set of purple carrot accessions to characterize anthocyanins, phenolic...

Descripción completa

Detalles Bibliográficos
Autores principales: Seth, Romit, Fiecke, Chelsey, Guoying, Ma, Perkins-Veazie, Penelope, Cavagnaro, Pablo, Ferruzzi, Mario G., Iorizzo, Massimo
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/23804
https://www.sciencedirect.com/science/article/pii/S2590157525006546
https://doi.org/10.1016/j.fochx.2025.102807
_version_ 1855038823069646848
author Seth, Romit
Fiecke, Chelsey
Guoying, Ma
Perkins-Veazie, Penelope
Cavagnaro, Pablo
Ferruzzi, Mario G.
Iorizzo, Massimo
author_browse Cavagnaro, Pablo
Ferruzzi, Mario G.
Fiecke, Chelsey
Guoying, Ma
Iorizzo, Massimo
Perkins-Veazie, Penelope
Seth, Romit
author_facet Seth, Romit
Fiecke, Chelsey
Guoying, Ma
Perkins-Veazie, Penelope
Cavagnaro, Pablo
Ferruzzi, Mario G.
Iorizzo, Massimo
author_sort Seth, Romit
collection INTA Digital
description Purple carrot is an important source of anthocyanins as a natural food dye, yet research advancements in selecting purple cultivars with improved anthocyanin extractable yield and stability remain limited. This study used a large set of purple carrot accessions to characterize anthocyanins, phenolics, and flavonoids, their relationship to tissue-specific accumulation, extraction efficiency, color density, and color stability. A Fourier-transform near-infrared (FT-NIR) spectroscopy method was also developed to evaluate these metabolites. Fully purple roots showed the highest anthocyanin content and extraction efficiency. Non-acylated anthocyanin Cy3XG was identified as a limiting step in the biosynthesis of stable acylated anthocyanins. The sinapoyl-containing glycoside Cy3XSGG was the primary contributor to color density. Ten FT-NIR prediction models were developed to evaluate anthocyanins, chlorogenic, and caffeic acids. These findings support the selection of purple carrot with stable and uniform anthocyanin production and perform quality control in the natural colorant industry.
format info:ar-repo/semantics/artículo
id INTA23804
institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher Elsevier
publisherStr Elsevier
record_format dspace
spelling INTA238042025-09-15T11:17:35Z Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy Seth, Romit Fiecke, Chelsey Guoying, Ma Perkins-Veazie, Penelope Cavagnaro, Pablo Ferruzzi, Mario G. Iorizzo, Massimo Zanahoria Variedades Compuestos Fenólicos Antocianinas Flavonoides Espectroscopia Carrots Varieties Phenolic Compounds Anthocyanins Flavonoids Spectroscopy Zanahoria Púrpura Purple Carrot Purple carrot is an important source of anthocyanins as a natural food dye, yet research advancements in selecting purple cultivars with improved anthocyanin extractable yield and stability remain limited. This study used a large set of purple carrot accessions to characterize anthocyanins, phenolics, and flavonoids, their relationship to tissue-specific accumulation, extraction efficiency, color density, and color stability. A Fourier-transform near-infrared (FT-NIR) spectroscopy method was also developed to evaluate these metabolites. Fully purple roots showed the highest anthocyanin content and extraction efficiency. Non-acylated anthocyanin Cy3XG was identified as a limiting step in the biosynthesis of stable acylated anthocyanins. The sinapoyl-containing glycoside Cy3XSGG was the primary contributor to color density. Ten FT-NIR prediction models were developed to evaluate anthocyanins, chlorogenic, and caffeic acids. These findings support the selection of purple carrot with stable and uniform anthocyanin production and perform quality control in the natural colorant industry. EEA Mendoza Fil: Seth, Romit. North Carolina State University. Plants for Human Health Institute; Estados Unidos Fil: Fiecke, Chelsey. University of Arkansas for Medical Sciences. Arkansas Children's Nutrition Center and Department of Pediatrics; Estados Unidos Fil: Guoying, Ma. North Carolina State University. Plants for Human Health Institute; Estados Unidos Fil: Perkins-Veazie, Penelope. North Carolina State University. Plants for Human Health Institute; Estados Unidos Fil: Perkins-Veazie, Penelope. North Carolina State University. Department of Horticultural Science; Estados Unidos Fil: Cavagnaro, Pablo Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Cavagnaro, Pablo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ferruzzi, Mario G. University of Arkansas for Medical Sciences. Arkansas Children's Nutrition Center and Department of Pediatrics; Estados Unidos Fil: Iorizzo, Massimo. North Carolina State University. Plants for Human Health Institute; Estados Unidos Fil: Iorizzo, Massimo. North Carolina State University. Department of Horticultural Science; Estados Unidos 2025-09-15T11:12:32Z 2025-09-15T11:12:32Z 2025-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23804 https://www.sciencedirect.com/science/article/pii/S2590157525006546 2590-1575 https://doi.org/10.1016/j.fochx.2025.102807 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Food Chemistry: X 29 : 102807. (July 2025)
spellingShingle Zanahoria
Variedades
Compuestos Fenólicos
Antocianinas
Flavonoides
Espectroscopia
Carrots
Varieties
Phenolic Compounds
Anthocyanins
Flavonoids
Spectroscopy
Zanahoria Púrpura
Purple Carrot
Seth, Romit
Fiecke, Chelsey
Guoying, Ma
Perkins-Veazie, Penelope
Cavagnaro, Pablo
Ferruzzi, Mario G.
Iorizzo, Massimo
Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy
title Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy
title_full Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy
title_fullStr Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy
title_full_unstemmed Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy
title_short Characterization of anthocyanins, phenolics, and flavonoids in a global carrot collection through application of chemometrics and FT-NIR spectroscopy
title_sort characterization of anthocyanins phenolics and flavonoids in a global carrot collection through application of chemometrics and ft nir spectroscopy
topic Zanahoria
Variedades
Compuestos Fenólicos
Antocianinas
Flavonoides
Espectroscopia
Carrots
Varieties
Phenolic Compounds
Anthocyanins
Flavonoids
Spectroscopy
Zanahoria Púrpura
Purple Carrot
url http://hdl.handle.net/20.500.12123/23804
https://www.sciencedirect.com/science/article/pii/S2590157525006546
https://doi.org/10.1016/j.fochx.2025.102807
work_keys_str_mv AT sethromit characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy
AT fieckechelsey characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy
AT guoyingma characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy
AT perkinsveaziepenelope characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy
AT cavagnaropablo characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy
AT ferruzzimariog characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy
AT iorizzomassimo characterizationofanthocyaninsphenolicsandflavonoidsinaglobalcarrotcollectionthroughapplicationofchemometricsandftnirspectroscopy