Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world
Sugar beet is an important agricultural crop product that has been produced and consumed worldwide since the eighteenth century and can adapt to various climatic and soil conditions. The two fundamental building blocks of any crop improvement program are germplasm resources, which contain genetic di...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/23799 https://link.springer.com/article/10.1007/s10722-024-02148-3 https://doi.org/10.1007/s10722-024-02148-3 |
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| author | Sadık, Gökhan Yildiz, Mehtap Taşkın, Bilgin Koçak, Metin Cavagnaro, Pablo Baloch, Faheem Shehzad |
| author_browse | Baloch, Faheem Shehzad Cavagnaro, Pablo Koçak, Metin Sadık, Gökhan Taşkın, Bilgin Yildiz, Mehtap |
| author_facet | Sadık, Gökhan Yildiz, Mehtap Taşkın, Bilgin Koçak, Metin Cavagnaro, Pablo Baloch, Faheem Shehzad |
| author_sort | Sadık, Gökhan |
| collection | INTA Digital |
| description | Sugar beet is an important agricultural crop product that has been produced and consumed worldwide since the eighteenth century and can adapt to various climatic and soil conditions. The two fundamental building blocks of any crop improvement program are germplasm resources, which contain genetic diversity and phenotypic expression of desired traits. In this study, a total of 58 sugar beet genotypes including 12 from Turkey, 4 from India, 12 from the United States of America, 16 from Iran, 12 from England and Beta vulgaris L. subsp. maritima L. Arcang. as wild species were characterized using 15 inter-primer binding site (iPBS) markers that produced intense and polymorphic bands in the germplasm library. Using these 15 iPBS markers, 102 polymorphic bands were produced and the average number of polymorphic bands was determined as 6.8. Polymorphism information content (PIC) values ranged between 0.58 and 0.83, and the average PIC value was found to be 0.70. It was determined that the most genetically different genotypes were PI 590697-US11 and PI 171508-TR8, with a distance of 0.73. Clustering algorithms Unweighted Pair Group Method Algorithm (UPGMA) and Principal Coordinate Algorithm (PCoA) confirmed that genotypes are an important factor in clustering, and STRUCTURE analysis divided sugar beet gene resources into six populations. Also, the analysis of molecular variance (AMOVA) showed that there was 8% variance among populations and 92% variance within populations. This is the first study to investigate the genetic diversity and population structure of sugar beet germplasm using the iPBS-retrotransposon marker system. The results of this research emphasized that iPBS markers are very successful and effective in examining the genetic diversity of sugar beet germplasm. The results obtained in this study provide a theoretical basis for future selection and breeding of superior sugar beet germplasm sources. |
| format | Artículo |
| id | INTA23799 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA237992025-09-12T14:40:04Z Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world Sadık, Gökhan Yildiz, Mehtap Taşkın, Bilgin Koçak, Metin Cavagnaro, Pablo Baloch, Faheem Shehzad Remolacha Azucarera Germoplasma Marcadores Genéticos Diversidad genética (recurso) Sugar Beet Beta vulgaris Germplasm Genetic Markers Genetic Diversity (resource) Marcadores Moleculares Sugar beet is an important agricultural crop product that has been produced and consumed worldwide since the eighteenth century and can adapt to various climatic and soil conditions. The two fundamental building blocks of any crop improvement program are germplasm resources, which contain genetic diversity and phenotypic expression of desired traits. In this study, a total of 58 sugar beet genotypes including 12 from Turkey, 4 from India, 12 from the United States of America, 16 from Iran, 12 from England and Beta vulgaris L. subsp. maritima L. Arcang. as wild species were characterized using 15 inter-primer binding site (iPBS) markers that produced intense and polymorphic bands in the germplasm library. Using these 15 iPBS markers, 102 polymorphic bands were produced and the average number of polymorphic bands was determined as 6.8. Polymorphism information content (PIC) values ranged between 0.58 and 0.83, and the average PIC value was found to be 0.70. It was determined that the most genetically different genotypes were PI 590697-US11 and PI 171508-TR8, with a distance of 0.73. Clustering algorithms Unweighted Pair Group Method Algorithm (UPGMA) and Principal Coordinate Algorithm (PCoA) confirmed that genotypes are an important factor in clustering, and STRUCTURE analysis divided sugar beet gene resources into six populations. Also, the analysis of molecular variance (AMOVA) showed that there was 8% variance among populations and 92% variance within populations. This is the first study to investigate the genetic diversity and population structure of sugar beet germplasm using the iPBS-retrotransposon marker system. The results of this research emphasized that iPBS markers are very successful and effective in examining the genetic diversity of sugar beet germplasm. The results obtained in this study provide a theoretical basis for future selection and breeding of superior sugar beet germplasm sources. EEA Mendoza Fil: Sadık, Gökhan. Van Yuzuncu Yil University. Faculty of Agriculture. Department of Agricultural Biotechnology; Turquía Fil: Yıldız, Mehtap. Van Yuzuncu Yil University. Faculty of Agriculture. Department of Agricultural Biotechnology; Turquía Fil: Taşkın, Bilgin. Van Yuzuncu Yil University. Faculty of Agriculture. Department of Agricultural Biotechnology; Turquía Fil: Koçak, Metin. Van Yuzuncu Yil University. Faculty of Agriculture. Department of Agricultural Biotechnology; Turquía Fil: Cavagnaro, Pablo Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Cavagnaro, Pablo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavagnaro, Pablo Federico. University of Agriculture in Krakow. Faculty of Biotechnology and Horticulture. Department of Plant Biology, and Biotechnology; Polonia Fil: Baloch, Faheem Shehzad. Mersin University. Faculty of Sciences; Turquía 2025-09-12T14:37:24Z 2025-09-12T14:37:24Z 2025-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23799 https://link.springer.com/article/10.1007/s10722-024-02148-3 0925-9864 1573-5109 https://doi.org/10.1007/s10722-024-02148-3 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Genetic Resources and Crop Evolution 72 (3) : 3039-3049. (2025) |
| spellingShingle | Remolacha Azucarera Germoplasma Marcadores Genéticos Diversidad genética (recurso) Sugar Beet Beta vulgaris Germplasm Genetic Markers Genetic Diversity (resource) Marcadores Moleculares Sadık, Gökhan Yildiz, Mehtap Taşkın, Bilgin Koçak, Metin Cavagnaro, Pablo Baloch, Faheem Shehzad Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world |
| title | Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world |
| title_full | Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world |
| title_fullStr | Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world |
| title_full_unstemmed | Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world |
| title_short | Application of iPBS-retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet (Beta vulgaris) germplasm from different regions of the world |
| title_sort | application of ipbs retrotransposons markers for the assessment of genetic diversity and population structure among sugar beet beta vulgaris germplasm from different regions of the world |
| topic | Remolacha Azucarera Germoplasma Marcadores Genéticos Diversidad genética (recurso) Sugar Beet Beta vulgaris Germplasm Genetic Markers Genetic Diversity (resource) Marcadores Moleculares |
| url | http://hdl.handle.net/20.500.12123/23799 https://link.springer.com/article/10.1007/s10722-024-02148-3 https://doi.org/10.1007/s10722-024-02148-3 |
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