Molecular identification and characterization of two rubber dandelion amalgaviruses
The Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting v...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2348 https://doi.org/10.1101/229443 |
| _version_ | 1855034947158409216 |
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| author | Debat, Humberto Julio Zinan Luo Iaffaldano, Brian J. Xiaofeng, Zhuang Cornish, Katrina |
| author_browse | Cornish, Katrina Debat, Humberto Julio Iaffaldano, Brian J. Xiaofeng, Zhuang Zinan Luo |
| author_facet | Debat, Humberto Julio Zinan Luo Iaffaldano, Brian J. Xiaofeng, Zhuang Cornish, Katrina |
| author_sort | Debat, Humberto Julio |
| collection | INTA Digital |
| description | The Amalgaviridae family is composed of persistent viruses that share the genome architecture of
Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been
assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses
with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and
characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The
sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping
ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic
insights based on the detected virus sequences suggest them to be members of two new species within the
Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a
dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated
with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 &
2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural
rubber crop. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2348 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA23482019-01-25T18:43:54Z Molecular identification and characterization of two rubber dandelion amalgaviruses Debat, Humberto Julio Zinan Luo Iaffaldano, Brian J. Xiaofeng, Zhuang Cornish, Katrina Taraxacum koksaghyz Virus de las Plantas dsRNA Viruses Plant Viruses Rubber Dandelion Amalgavirus Diente de León The Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic insights based on the detected virus sequences suggest them to be members of two new species within the Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 & 2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural rubber crop. Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Zinan Luo. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos Fil: Iaffaldano, Brian J. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos Fil: Xiaofeng Zhuang. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos Fil: Cornish, Katrina. Ohio State University. Department of Horticulture and Crop Science; Estados Unidos 2018-05-08T13:34:31Z 2018-05-08T13:34:31Z 2017-12-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2348 https://doi.org/10.1101/229443 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioRxiv : the preprint server for biology. (December 5, 2017) |
| spellingShingle | Taraxacum koksaghyz Virus de las Plantas dsRNA Viruses Plant Viruses Rubber Dandelion Amalgavirus Diente de León Debat, Humberto Julio Zinan Luo Iaffaldano, Brian J. Xiaofeng, Zhuang Cornish, Katrina Molecular identification and characterization of two rubber dandelion amalgaviruses |
| title | Molecular identification and characterization of two rubber dandelion amalgaviruses |
| title_full | Molecular identification and characterization of two rubber dandelion amalgaviruses |
| title_fullStr | Molecular identification and characterization of two rubber dandelion amalgaviruses |
| title_full_unstemmed | Molecular identification and characterization of two rubber dandelion amalgaviruses |
| title_short | Molecular identification and characterization of two rubber dandelion amalgaviruses |
| title_sort | molecular identification and characterization of two rubber dandelion amalgaviruses |
| topic | Taraxacum koksaghyz Virus de las Plantas dsRNA Viruses Plant Viruses Rubber Dandelion Amalgavirus Diente de León |
| url | http://hdl.handle.net/20.500.12123/2348 https://doi.org/10.1101/229443 |
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