Molecular identification and characterization of two rubber dandelion amalgaviruses
The Amalgaviridae family is composed of persistent viruses that share the genome architecture of Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been assigned to this family, presenting only four recognized species, corresponding to plant infecting v...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2348 https://doi.org/10.1101/229443 |
| Sumario: | The Amalgaviridae family is composed of persistent viruses that share the genome architecture of
Totiviridae and gene evolutionary resemblance to Partitiviridae. A single Amalgavirus genus has been
assigned to this family, presenting only four recognized species, corresponding to plant infecting viruses
with dsRNA monopartite genomes of ca. 3.4 kb. Here, we present the genomic identification and
characterization of two novel viruses detected in rubber dandelion (Taraxacum kok-saghyz). The
sequenced isolates presented genomes of 3,409 and 3,413 nt long, including two partially overlapping
ORFs encoding a putative coat protein and an RNA-dependent RNA polymerase (RdRP). Phylogenetic
insights based on the detected virus sequences suggest them to be members of two new species within the
Amalgavirus genus. Multiple independent RNAseq data suggest that the identified viruses have a
dynamic distribution and low relative RNA levels in infected plants. Virus presence was not associated
with any apparent symptoms on the plant hosts. We propose the names rubber dandelion latent virus 1 &
2 to the detected amalgaviruses; the first viruses to be associated to this emergent and sustainable natural
rubber crop. |
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