Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
Bread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be u...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://link.springer.com/article/10.3103/S0095452717040028 http://hdl.handle.net/20.500.12123/2265 https://doi.org/10.3103/S0095452717040028 |
| _version_ | 1855483024584474624 |
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| author | Lombardo, Lucio Nisi, María Mercedes Salines, Nicolas Ghione, Celina Elena Helguera, Marcelo |
| author_browse | Ghione, Celina Elena Helguera, Marcelo Lombardo, Lucio Nisi, María Mercedes Salines, Nicolas |
| author_facet | Lombardo, Lucio Nisi, María Mercedes Salines, Nicolas Ghione, Celina Elena Helguera, Marcelo |
| author_sort | Lombardo, Lucio |
| collection | INTA Digital |
| description | Bread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be used for the discovery of valuable mutants for basic research and breeding purposes. In this study, we report the identification of 27 HMW-GS M3 mutants based on SDS-PAGE patterns from an EMS mutagenized population of the cultivar Baguette Premium 11. Nine mutations were detected in Ax2*, five in Bx7, four in By8, six in Dx5 and three in Dy10 subunit. Two Ax2* null mutants were characterized at molecular level finding in both cases premature stop codons associated. EMS would tend to generate more premature stop codons in glutenins genes than in others because these have a high frequency of glutamine codons. This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed. |
| format | Artículo |
| id | INTA2265 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22652018-06-21T17:41:18Z Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) Lombardo, Lucio Nisi, María Mercedes Salines, Nicolas Ghione, Celina Elena Helguera, Marcelo Trigo Variedades Gluteninas Peso Molecular Wheat Varieties Glutenins Molecular Weight Trigo Pan Bread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be used for the discovery of valuable mutants for basic research and breeding purposes. In this study, we report the identification of 27 HMW-GS M3 mutants based on SDS-PAGE patterns from an EMS mutagenized population of the cultivar Baguette Premium 11. Nine mutations were detected in Ax2*, five in Bx7, four in By8, six in Dx5 and three in Dy10 subunit. Two Ax2* null mutants were characterized at molecular level finding in both cases premature stop codons associated. EMS would tend to generate more premature stop codons in glutenins genes than in others because these have a high frequency of glutamine codons. This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed. EEA Marcos Juárez Fil: Lombardo, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Nisi, María Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina Fil: Salines, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Área Mejoramiento Genético Vegetal; Argentina Fil: Ghione, Celina Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina 2018-04-17T18:01:32Z 2018-04-17T18:01:32Z 2017-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.3103/S0095452717040028 http://hdl.handle.net/20.500.12123/2265 0095-4527 1934-9440 https://doi.org/10.3103/S0095452717040028 eng info:eu-repo/semantics/restrictedAccess application/pdf Cytology and genetics 51 (4) : 305–314. (July 2017) |
| spellingShingle | Trigo Variedades Gluteninas Peso Molecular Wheat Varieties Glutenins Molecular Weight Trigo Pan Lombardo, Lucio Nisi, María Mercedes Salines, Nicolas Ghione, Celina Elena Helguera, Marcelo Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) |
| title | Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) |
| title_full | Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) |
| title_fullStr | Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) |
| title_full_unstemmed | Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) |
| title_short | Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.) |
| title_sort | identification of novel high molecular weight glutenin subunit mutants in bread wheat triticum aestivum l |
| topic | Trigo Variedades Gluteninas Peso Molecular Wheat Varieties Glutenins Molecular Weight Trigo Pan |
| url | https://link.springer.com/article/10.3103/S0095452717040028 http://hdl.handle.net/20.500.12123/2265 https://doi.org/10.3103/S0095452717040028 |
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