Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma
Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study ai...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/22647 https://www.mdpi.com/1999-4915/17/6/744 https://doi.org/10.3390/v17060744 |
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| author | Lopez, Sofia Álvarez, Ignacio Andreoli, V. Delgado, S. Pérez, S. Pereyra, Susana Beatriz Romeo, Florencia Grolli, S. Verna, Andrea Elizabeth |
| author_browse | Andreoli, V. Delgado, S. Grolli, S. Lopez, Sofia Pereyra, Susana Beatriz Pérez, S. Romeo, Florencia Verna, Andrea Elizabeth Álvarez, Ignacio |
| author_facet | Lopez, Sofia Álvarez, Ignacio Andreoli, V. Delgado, S. Pérez, S. Pereyra, Susana Beatriz Romeo, Florencia Grolli, S. Verna, Andrea Elizabeth |
| author_sort | Lopez, Sofia |
| collection | INTA Digital |
| description | Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet-rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate–early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFN-γ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments. |
| format | Artículo |
| id | INTA22647 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA226472025-06-12T10:14:34Z Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma Lopez, Sofia Álvarez, Ignacio Andreoli, V. Delgado, S. Pérez, S. Pereyra, Susana Beatriz Romeo, Florencia Grolli, S. Verna, Andrea Elizabeth Ganado Bovino Expresión Génica Inmunomodulación Virus Herpes Virus Bovino Plasma Sanguíneo Cattle Gene Expression Immunomodulation Viruses Bovine Herpesvirus Blood Plasma Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet-rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate–early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFN-γ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments. EEA Balcarce Fil: López, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Álvarez, Ignacio. Swedish University of Agricultural Sciences. Department of Clinical Sciences; Suecia Fil: Andreoli, V. Forestali e Alimentari, Università di Torino. Dipartimento di Scienze Agrarie; Italia Fil: Delgado, S. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Pérez, S. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Pereyra, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Grolli, S. Università di Parma. Dipartimento di Scienze Medico Veterinarie; Italia Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina 2025-06-12T10:08:10Z 2025-06-12T10:08:10Z 2025-05-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22647 https://www.mdpi.com/1999-4915/17/6/744 1999-4915 https://doi.org/10.3390/v17060744 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) MDPI Viruses 17(6) : 744 (June 2025) |
| spellingShingle | Ganado Bovino Expresión Génica Inmunomodulación Virus Herpes Virus Bovino Plasma Sanguíneo Cattle Gene Expression Immunomodulation Viruses Bovine Herpesvirus Blood Plasma Lopez, Sofia Álvarez, Ignacio Andreoli, V. Delgado, S. Pérez, S. Pereyra, Susana Beatriz Romeo, Florencia Grolli, S. Verna, Andrea Elizabeth Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma |
| title | Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma |
| title_full | Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma |
| title_fullStr | Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma |
| title_full_unstemmed | Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma |
| title_short | Gene Expression Modulation in Bovine Endometrial Cells Infected with Gammaherpesvirus Type 4 and Exposed to Lipopolysaccharide in the Presence of Platelet-Rich Plasma |
| title_sort | gene expression modulation in bovine endometrial cells infected with gammaherpesvirus type 4 and exposed to lipopolysaccharide in the presence of platelet rich plasma |
| topic | Ganado Bovino Expresión Génica Inmunomodulación Virus Herpes Virus Bovino Plasma Sanguíneo Cattle Gene Expression Immunomodulation Viruses Bovine Herpesvirus Blood Plasma |
| url | http://hdl.handle.net/20.500.12123/22647 https://www.mdpi.com/1999-4915/17/6/744 https://doi.org/10.3390/v17060744 |
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