Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design
Synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG-ODNs) are immunostimulants widely used as vaccine adjuvants. This study evaluated the innate immune responses induced by four CpG-ODNs: [2395 (class C), 1826, 1018, and 2007 (class B)] in porcine and murine cells, r...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/22370 https://www.sciencedirect.com/science/article/abs/pii/S0034528825001663 https://doi.org/10.1016/j.rvsc.2025.105692 |
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| author | Vedelago, Giuliana Palacios, Luz M. Castell, Sofía D. Marin, Constanza Ruiz Moreno, Federico N. Felici, M. Esperanza Dho, Nicolás D. Allemandi, Daniel A. Palma, Santiago Daniel Moron, Victor Gabriel Bessone, Fernando Aníbal Alustiza, Fabrisio Eduardo Crespo, Maria Ines Maletto, Belkys Angélica |
| author_browse | Allemandi, Daniel A. Alustiza, Fabrisio Eduardo Bessone, Fernando Aníbal Castell, Sofía D. Crespo, Maria Ines Dho, Nicolás D. Felici, M. Esperanza Maletto, Belkys Angélica Marin, Constanza Moron, Victor Gabriel Palacios, Luz M. Palma, Santiago Daniel Ruiz Moreno, Federico N. Vedelago, Giuliana |
| author_facet | Vedelago, Giuliana Palacios, Luz M. Castell, Sofía D. Marin, Constanza Ruiz Moreno, Federico N. Felici, M. Esperanza Dho, Nicolás D. Allemandi, Daniel A. Palma, Santiago Daniel Moron, Victor Gabriel Bessone, Fernando Aníbal Alustiza, Fabrisio Eduardo Crespo, Maria Ines Maletto, Belkys Angélica |
| author_sort | Vedelago, Giuliana |
| collection | INTA Digital |
| description | Synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG-ODNs) are immunostimulants widely used as vaccine adjuvants. This study evaluated the innate immune responses induced by four CpG-ODNs: [2395 (class C), 1826, 1018, and 2007 (class B)] in porcine and murine cells, representing species of veterinary and preclinical interest, respectively. Porcine peripheral blood mononuclear cells and murine splenocytes were stimulated in vitro with each CpG-ODN, and the mRNA expression of IL-6, IL-12p40, IFN-β, IFN-γ, TNF, and TLR9 was assessed by RT-qPCR. In porcine cells, only CpG-ODN 2395 significantly upregulated IL-6, IL-12p40, and TLR9. In murine cells, all CpG-ODNs induced IL-6; CpG-ODNs 2395, 1826, and 1018 upregulated IL-12p40; and only CpG-ODN 2395 induced TLR9. Stimulation with CpG-ODNs 2395 and 1018 enhanced IFN-β and IFN-γ expression in porcine cells. In contrast, murine cells showed increased IFN-β levels in response to CpG-ODNs 2395 and 1826, and upregulated IFN-γ following stimulation with CpG-ODNs 2395, 1826, and 1018. TNF expression was exclusively induced in porcine cells by CpG-ODN 1018, 2395, and 2007. Notably, CpG-ODN 1826 exhibited species-specific activity in mice, inducing IL-6, IL-12p40, IFN-β, and IFN-γ. These findings reveal significant interspecies differences in CpG-ODN-induced immune responses, emphasizing the importance of species-specific considerations when selecting CpG-ODNs as vaccine adjuvants for preclinical and veterinary applications. |
| format | Artículo |
| id | INTA22370 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA223702026-01-05T10:55:33Z Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design Vedelago, Giuliana Palacios, Luz M. Castell, Sofía D. Marin, Constanza Ruiz Moreno, Federico N. Felici, M. Esperanza Dho, Nicolás D. Allemandi, Daniel A. Palma, Santiago Daniel Moron, Victor Gabriel Bessone, Fernando Aníbal Alustiza, Fabrisio Eduardo Crespo, Maria Ines Maletto, Belkys Angélica Vacuna Respuesta Inmunológica Coadyuvantes Cerdo Ratón Vaccines Immune Response Adjuvants Swine Mice Synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG-ODNs) are immunostimulants widely used as vaccine adjuvants. This study evaluated the innate immune responses induced by four CpG-ODNs: [2395 (class C), 1826, 1018, and 2007 (class B)] in porcine and murine cells, representing species of veterinary and preclinical interest, respectively. Porcine peripheral blood mononuclear cells and murine splenocytes were stimulated in vitro with each CpG-ODN, and the mRNA expression of IL-6, IL-12p40, IFN-β, IFN-γ, TNF, and TLR9 was assessed by RT-qPCR. In porcine cells, only CpG-ODN 2395 significantly upregulated IL-6, IL-12p40, and TLR9. In murine cells, all CpG-ODNs induced IL-6; CpG-ODNs 2395, 1826, and 1018 upregulated IL-12p40; and only CpG-ODN 2395 induced TLR9. Stimulation with CpG-ODNs 2395 and 1018 enhanced IFN-β and IFN-γ expression in porcine cells. In contrast, murine cells showed increased IFN-β levels in response to CpG-ODNs 2395 and 1826, and upregulated IFN-γ following stimulation with CpG-ODNs 2395, 1826, and 1018. TNF expression was exclusively induced in porcine cells by CpG-ODN 1018, 2395, and 2007. Notably, CpG-ODN 1826 exhibited species-specific activity in mice, inducing IL-6, IL-12p40, IFN-β, and IFN-γ. These findings reveal significant interspecies differences in CpG-ODN-induced immune responses, emphasizing the importance of species-specific considerations when selecting CpG-ODNs as vaccine adjuvants for preclinical and veterinary applications. EEA Marcos Juárez Fil: Vedelago, Giuliana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Vedelago, Giuliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Palacios, Luz M. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Palacios, Luz M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Castell, Sofía D. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Castell, Sofía D. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Marin, Constanza. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Marin, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Ruiz Moreno, Federico N. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Ruiz Moreno, Federico N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Felici, M. Esperanza. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Felici, M. Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Dho, Nicolás D. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Dho, Nicolás D. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Allemandi, Daniel A. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina Fil: Allemandi, Daniel A. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Allemandi, Daniel A. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Palma, Santiago D. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas; Argentina Fil: Palma, Santiago D. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Palma, Santiago D. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Moron, Victor Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Bessone, Fernando Aníbal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Crespo, Maria Ines. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina Fil: Maletto, Belkys Angélica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI); Argentina 2025-05-22T11:28:41Z 2025-05-22T11:28:41Z 2025-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22370 https://www.sciencedirect.com/science/article/abs/pii/S0034528825001663 0034-5288 1532-2661 https://doi.org/10.1016/j.rvsc.2025.105692 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Research in Veterinary Science 191 : 105692. (August 2025) |
| spellingShingle | Vacuna Respuesta Inmunológica Coadyuvantes Cerdo Ratón Vaccines Immune Response Adjuvants Swine Mice Vedelago, Giuliana Palacios, Luz M. Castell, Sofía D. Marin, Constanza Ruiz Moreno, Federico N. Felici, M. Esperanza Dho, Nicolás D. Allemandi, Daniel A. Palma, Santiago Daniel Moron, Victor Gabriel Bessone, Fernando Aníbal Alustiza, Fabrisio Eduardo Crespo, Maria Ines Maletto, Belkys Angélica Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design |
| title | Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design |
| title_full | Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design |
| title_fullStr | Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design |
| title_full_unstemmed | Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design |
| title_short | Differential innate immune activation by four different CpG-ODNs in porcine and murine cells: Implications for vaccine design |
| title_sort | differential innate immune activation by four different cpg odns in porcine and murine cells implications for vaccine design |
| topic | Vacuna Respuesta Inmunológica Coadyuvantes Cerdo Ratón Vaccines Immune Response Adjuvants Swine Mice |
| url | http://hdl.handle.net/20.500.12123/22370 https://www.sciencedirect.com/science/article/abs/pii/S0034528825001663 https://doi.org/10.1016/j.rvsc.2025.105692 |
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