Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants...
| Main Authors: | , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/2227 https://doi.org/10.1111/j.1570-7458.2010.01002.x |
| Summary: | DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann
(Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene
(Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants
were detected among the 307 samples analyzed. To better differentiate the size variants, further
digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide
sequences of the three-allele variants were obtained and single changes, insertions, deletions, and
EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean
heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed
to Hardy–Weinberg expectations. Significant differences between provinces and sampling
sites within these provinces, and among some populations were found. The average number of
insects exchanged among populations (Nm) was estimated and high values were observed between
Argentina and populations fromtwo African countries (Morocco and Kenya), Australia, and Hawaii
(Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these
results as well as on available bibliographical data. |
|---|