Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants...
| Main Authors: | , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/2227 https://doi.org/10.1111/j.1570-7458.2010.01002.x |
| _version_ | 1855483017212985344 |
|---|---|
| author | Lanzavecchia, Silvia Beatriz Remis, Maria Isabel Cladera, Jorge Luis Zandomeni, Ruben |
| author_browse | Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Remis, Maria Isabel Zandomeni, Ruben |
| author_facet | Lanzavecchia, Silvia Beatriz Remis, Maria Isabel Cladera, Jorge Luis Zandomeni, Ruben |
| author_sort | Lanzavecchia, Silvia Beatriz |
| collection | INTA Digital |
| description | DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann
(Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene
(Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants
were detected among the 307 samples analyzed. To better differentiate the size variants, further
digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide
sequences of the three-allele variants were obtained and single changes, insertions, deletions, and
EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean
heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed
to Hardy–Weinberg expectations. Significant differences between provinces and sampling
sites within these provinces, and among some populations were found. The average number of
insects exchanged among populations (Nm) was estimated and high values were observed between
Argentina and populations fromtwo African countries (Morocco and Kenya), Australia, and Hawaii
(Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these
results as well as on available bibliographical data. |
| format | Artículo |
| id | INTA2227 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22272019-01-22T18:33:27Z Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata Lanzavecchia, Silvia Beatriz Remis, Maria Isabel Cladera, Jorge Luis Zandomeni, Ruben Ceratitis Capitata Alcohol Deshidrogenasa Marcadores Genéticos Intrones Población Tephritidae Polimorfismo Genético Genetic Polymorphism Population Introns Genetic Markers Alcohol Dehydrogenase ADH1 Argentina DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy–Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations fromtwo African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Remis, Maria Isabel. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Genética de la Estructura Poblacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Zandomeni, Ruben. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Alta Complejidad; Argentina 2018-04-12T12:29:44Z 2018-04-12T12:29:44Z 2010-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2227 0013-8703 1570-7458 (Online version) https://doi.org/10.1111/j.1570-7458.2010.01002.x eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Entomologia experimentalis et applicata 136 (1) : 53–65. (July 2010) |
| spellingShingle | Ceratitis Capitata Alcohol Deshidrogenasa Marcadores Genéticos Intrones Población Tephritidae Polimorfismo Genético Genetic Polymorphism Population Introns Genetic Markers Alcohol Dehydrogenase ADH1 Argentina Lanzavecchia, Silvia Beatriz Remis, Maria Isabel Cladera, Jorge Luis Zandomeni, Ruben Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
| title | Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
| title_full | Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
| title_fullStr | Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
| title_full_unstemmed | Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
| title_short | Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata |
| title_sort | adh1 first intron polymorphisms in argentinean populations of ceratitis capitata |
| topic | Ceratitis Capitata Alcohol Deshidrogenasa Marcadores Genéticos Intrones Población Tephritidae Polimorfismo Genético Genetic Polymorphism Population Introns Genetic Markers Alcohol Dehydrogenase ADH1 Argentina |
| url | http://hdl.handle.net/20.500.12123/2227 https://doi.org/10.1111/j.1570-7458.2010.01002.x |
| work_keys_str_mv | AT lanzavecchiasilviabeatriz adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata AT remismariaisabel adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata AT claderajorgeluis adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata AT zandomeniruben adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata |