TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection
Background Plant viral infections disturb defense regulatory networks during tissue invasion. Emerging evidence demonstrates that a significant proportion of these alterations are mediated by hormone imbalances. Although the DELLA proteins have been reported to be central players in hormone cross-t...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2211 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0210-x |
| _version_ | 1855483014188892160 |
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| author | Rodriguez, Maria Cecilia Conti, Gabriela Zavallo, Diego Manacorda, Carlos Augusto Asurmendi, Sebastian |
| author_browse | Asurmendi, Sebastian Conti, Gabriela Manacorda, Carlos Augusto Rodriguez, Maria Cecilia Zavallo, Diego |
| author_facet | Rodriguez, Maria Cecilia Conti, Gabriela Zavallo, Diego Manacorda, Carlos Augusto Asurmendi, Sebastian |
| author_sort | Rodriguez, Maria Cecilia |
| collection | INTA Digital |
| description | Background
Plant viral infections disturb defense regulatory networks during tissue invasion. Emerging evidence demonstrates that a significant proportion of these alterations are mediated by hormone imbalances. Although the DELLA proteins have been reported to be central players in hormone cross-talk, their role in the modulation of hormone signaling during virus infections remains unknown.
Results
This work revealed that TMV-Cg coat protein (CgCP) suppresses the salicylic acid (SA) signaling pathway without altering defense hormone SA or jasmonic acid (JA) levels in Arabidopsis thaliana. Furthermore, it was observed that the expression of CgCP reduces plant growth and delays the timing of floral transition. Quantitative RT-qPCR analysis of DELLA target genes showed that CgCP alters relative expression of several target genes, indicating that the DELLA proteins mediate transcriptional changes produced by CgCP expression. Analyses by fluorescence confocal microscopy showed that CgCP stabilizes DELLA proteins accumulation in the presence of gibberellic acid (GA) and that the DELLA proteins are also stabilized during TMV-Cg virus infections. Moreover, DELLA proteins negatively modulated defense transcript profiles during TMV-Cg infection. As a result, TMV-Cg accumulation was significantly reduced in the quadruple-DELLA mutant Arabidopsis plants compared to wild type plants.
Conclusions
Taken together, these results demonstrate that CgCP negatively regulates the salicylic acid-mediated defense pathway by stabilizing the DELLA proteins during Arabidopsis thaliana viral infection, suggesting that CgCP alters the stability of DELLAs as a mechanism of negative modulation of antiviral defense responses. |
| format | Artículo |
| id | INTA2211 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22112018-04-10T13:37:51Z TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection Rodriguez, Maria Cecilia Conti, Gabriela Zavallo, Diego Manacorda, Carlos Augusto Asurmendi, Sebastian Tobamovirus del Mosaico del Tabaco Proteínas Virales Arabidopsis Thaliana Ácido Salicílico Virosis Infección Respuesta Inmunológica Immune Response Infection Viroses Salicylic Acids Viral Proteins Tobacco Mosaic Tobamovirus DELLA Proteins SA Signaling Background Plant viral infections disturb defense regulatory networks during tissue invasion. Emerging evidence demonstrates that a significant proportion of these alterations are mediated by hormone imbalances. Although the DELLA proteins have been reported to be central players in hormone cross-talk, their role in the modulation of hormone signaling during virus infections remains unknown. Results This work revealed that TMV-Cg coat protein (CgCP) suppresses the salicylic acid (SA) signaling pathway without altering defense hormone SA or jasmonic acid (JA) levels in Arabidopsis thaliana. Furthermore, it was observed that the expression of CgCP reduces plant growth and delays the timing of floral transition. Quantitative RT-qPCR analysis of DELLA target genes showed that CgCP alters relative expression of several target genes, indicating that the DELLA proteins mediate transcriptional changes produced by CgCP expression. Analyses by fluorescence confocal microscopy showed that CgCP stabilizes DELLA proteins accumulation in the presence of gibberellic acid (GA) and that the DELLA proteins are also stabilized during TMV-Cg virus infections. Moreover, DELLA proteins negatively modulated defense transcript profiles during TMV-Cg infection. As a result, TMV-Cg accumulation was significantly reduced in the quadruple-DELLA mutant Arabidopsis plants compared to wild type plants. Conclusions Taken together, these results demonstrate that CgCP negatively regulates the salicylic acid-mediated defense pathway by stabilizing the DELLA proteins during Arabidopsis thaliana viral infection, suggesting that CgCP alters the stability of DELLAs as a mechanism of negative modulation of antiviral defense responses. Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-04-10T13:20:26Z 2018-04-10T13:20:26Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2211 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0210-x 1471-2229 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC plant biology 14 : 210. (2014) |
| spellingShingle | Tobamovirus del Mosaico del Tabaco Proteínas Virales Arabidopsis Thaliana Ácido Salicílico Virosis Infección Respuesta Inmunológica Immune Response Infection Viroses Salicylic Acids Viral Proteins Tobacco Mosaic Tobamovirus DELLA Proteins SA Signaling Rodriguez, Maria Cecilia Conti, Gabriela Zavallo, Diego Manacorda, Carlos Augusto Asurmendi, Sebastian TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection |
| title | TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection |
| title_full | TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection |
| title_fullStr | TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection |
| title_full_unstemmed | TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection |
| title_short | TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thaliana viral infection |
| title_sort | tmv cg coat protein stabilizes della proteins and in turn negatively modulates salicylic acid mediated defense pathway during arabidopsis thaliana viral infection |
| topic | Tobamovirus del Mosaico del Tabaco Proteínas Virales Arabidopsis Thaliana Ácido Salicílico Virosis Infección Respuesta Inmunológica Immune Response Infection Viroses Salicylic Acids Viral Proteins Tobacco Mosaic Tobamovirus DELLA Proteins SA Signaling |
| url | http://hdl.handle.net/20.500.12123/2211 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0210-x |
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