P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum
Background/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caus...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21518 https://www.mdpi.com/2076-393X/13/2/162 https://doi.org/10.3390/vaccines13020162 |
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| author | Mira, Anabela Garro, Carlos Javier De Alba, Paloma Monti, Demián Esteban Lang, Maria Cecilia Vivas, Alejandro Medina, Esteban Franco, Juan Cruz Gutierrez, Álvaro Schnittger, Leonhard Wigdorovitz, Andres Parreño, Gladys Viviana Bok, Marina |
| author_browse | Bok, Marina De Alba, Paloma Franco, Juan Cruz Garro, Carlos Javier Gutierrez, Álvaro Lang, Maria Cecilia Medina, Esteban Mira, Anabela Monti, Demián Esteban Parreño, Gladys Viviana Schnittger, Leonhard Vivas, Alejandro Wigdorovitz, Andres |
| author_facet | Mira, Anabela Garro, Carlos Javier De Alba, Paloma Monti, Demián Esteban Lang, Maria Cecilia Vivas, Alejandro Medina, Esteban Franco, Juan Cruz Gutierrez, Álvaro Schnittger, Leonhard Wigdorovitz, Andres Parreño, Gladys Viviana Bok, Marina |
| author_sort | Mira, Anabela |
| collection | INTA Digital |
| description | Background/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caused by C. parvum. Methods: A recombinant immunogen comprising the p23 protein fused to the antigen-presenting cell homing (APCH) molecule was expressed using the baculovirus system. Hens were immunized with the APCH-p23 immunogen, and the resulting IgY was spray-dried for treatment use. Eight newborn calves were included in the study and received commercial colostrum within the first 12 h of life. Four calves were treated with 20 g of powdered egg containing IgY (p23-specific IgY titer of 256 in milk) twice daily for 7 days. The remaining four calves received regular non-supplemented milk. All calves were orally infected with 6 million oocysts and monitored for 21 days. Results: Calves treated with p23-specific IgY exhibited significantly reduced diarrhea duration (3.5 vs. 7.5 days; p = 0.0397) and oocyst shedding duration (6.50 vs. 12 days; p = 0.0089). In addition, the total number of excreted oocysts, as measured by the change of the area under the curve (AUC), was significantly reduced in the treated group (14.25 vs. 33.45; p = 0.0117). Although the onset of diarrhea was delayed (3.5 to 6.5 days post-infection; p = 0.1840), and diarrhea severity was reduced (24.25 to 17 AUC; p = 0.1236), both parameters were not statistically significant. Conclusions: P23-specific IgY antibodies effectively reduced the C. parvum-induced duration of diarrhea in experimentally infected calves. These findings highlight the potential of this passive treatment as a promising strategy for controlling neonatal calf diarrhea. |
| format | Artículo |
| id | INTA21518 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA215182025-02-28T14:03:26Z P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum Mira, Anabela Garro, Carlos Javier De Alba, Paloma Monti, Demián Esteban Lang, Maria Cecilia Vivas, Alejandro Medina, Esteban Franco, Juan Cruz Gutierrez, Álvaro Schnittger, Leonhard Wigdorovitz, Andres Parreño, Gladys Viviana Bok, Marina Cryptosporidium parvum Calves Diarrhoea Oocysts Ternero Diarrea Oocisto Background/Objectives: Cryptosporidium parvum is a zoonotic enteroparasite causing severe diarrhea in newborn calves, leading to significant economic losses in dairy and beef farming. This study aimed to evaluate whether C. parvum p23-specific IgY antibodies could control neonatal calf diarrhea caused by C. parvum. Methods: A recombinant immunogen comprising the p23 protein fused to the antigen-presenting cell homing (APCH) molecule was expressed using the baculovirus system. Hens were immunized with the APCH-p23 immunogen, and the resulting IgY was spray-dried for treatment use. Eight newborn calves were included in the study and received commercial colostrum within the first 12 h of life. Four calves were treated with 20 g of powdered egg containing IgY (p23-specific IgY titer of 256 in milk) twice daily for 7 days. The remaining four calves received regular non-supplemented milk. All calves were orally infected with 6 million oocysts and monitored for 21 days. Results: Calves treated with p23-specific IgY exhibited significantly reduced diarrhea duration (3.5 vs. 7.5 days; p = 0.0397) and oocyst shedding duration (6.50 vs. 12 days; p = 0.0089). In addition, the total number of excreted oocysts, as measured by the change of the area under the curve (AUC), was significantly reduced in the treated group (14.25 vs. 33.45; p = 0.0117). Although the onset of diarrhea was delayed (3.5 to 6.5 days post-infection; p = 0.1840), and diarrhea severity was reduced (24.25 to 17 AUC; p = 0.1236), both parameters were not statistically significant. Conclusions: P23-specific IgY antibodies effectively reduced the C. parvum-induced duration of diarrhea in experimentally infected calves. These findings highlight the potential of this passive treatment as a promising strategy for controlling neonatal calf diarrhea. Instituto de Patobiología Fil: Mira, Anabela. Bioinnovo SA; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Monti, Demian Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Lang, Maria Cecilia. Bioinnovo SA; Argentina Fil: Vivas, Alejandro. Bioinnovo SA; Argentina Fil: Medina, Esteban. Bioinnovo SA; Argentina Fil: Franco, Juan Cruz. Bioinnovo SA; Argentina Fil: Gutierrez, Álvaro. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina 2025-02-28T13:55:53Z 2025-02-28T13:55:53Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21518 https://www.mdpi.com/2076-393X/13/2/162 2076-393X https://doi.org/10.3390/vaccines13020162 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repograntAgreement/INTA/2023-PD-L01-I118, Innovaciones en los procesos de crianza, recría y vaca en transición de rodeos lecheros info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Vaccines 13 (2) : 162 (February 2025) |
| spellingShingle | Cryptosporidium parvum Calves Diarrhoea Oocysts Ternero Diarrea Oocisto Mira, Anabela Garro, Carlos Javier De Alba, Paloma Monti, Demián Esteban Lang, Maria Cecilia Vivas, Alejandro Medina, Esteban Franco, Juan Cruz Gutierrez, Álvaro Schnittger, Leonhard Wigdorovitz, Andres Parreño, Gladys Viviana Bok, Marina P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum |
| title | P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum |
| title_full | P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum |
| title_fullStr | P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum |
| title_full_unstemmed | P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum |
| title_short | P23-Specific IgY significantly reduces diarrhea and oocyst shedding in calves experimentally infected with Cryptosporidium parvum |
| title_sort | p23 specific igy significantly reduces diarrhea and oocyst shedding in calves experimentally infected with cryptosporidium parvum |
| topic | Cryptosporidium parvum Calves Diarrhoea Oocysts Ternero Diarrea Oocisto |
| url | http://hdl.handle.net/20.500.12123/21518 https://www.mdpi.com/2076-393X/13/2/162 https://doi.org/10.3390/vaccines13020162 |
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