Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1
Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/21500 https://www.sciencedirect.com/science/article/abs/pii/S0042682225000145 https://doi.org/10.1016/j.virol.2025.110402 |
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| author | Kornuta, Claudia Alejandra Bidart, Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Tribulatti, María Virginia Campetella, Oscar Prato, Cecilia A. Carabelli, Julieta Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines Langellotti, Cecilia Ana |
| author_browse | Bidart, Juan Esteban Campetella, Oscar Carabelli, Julieta Cheuquepan Valenzuel, Felipe Andres Gammella, Mariela Hecker, Yanina Kornuta, Claudia Alejandra Langellotti, Cecilia Ana Moore, Prando Dadin Prato, Cecilia A. Quattrocchi, Valeria Soria, Ivana Tribulatti, María Virginia Zamorano, Patricia Ines |
| author_facet | Kornuta, Claudia Alejandra Bidart, Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Tribulatti, María Virginia Campetella, Oscar Prato, Cecilia A. Carabelli, Julieta Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines Langellotti, Cecilia Ana |
| author_sort | Kornuta, Claudia Alejandra |
| collection | INTA Digital |
| description | Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide™ GEL01 PR (GEL01) and introduced Galectin-8 (Gal-8), a lectin considered a novel adjuvant due to its immunostimulatory effects, into the formulation.
Animals were vaccinated with pCIgD, pCIgD with Gal-8 (pCIgD-Gal-8), pCIgD with Gal-8 and GEL01 (pCIgD-Gal-8-GEL01), or the control plasmid pCIneo. The immune response was first assessed in a mouse model and then in bovines.
The results showed that combining Gal-8 and GEL01 with pCIgD modulated immune responses at both the humoral and cellular levels in both animal models.
This study evaluates the efficacy of a DNA vaccine with Gal-8 and GEL01 as potential adjuvants to enhance immune protection against BoAHV-1. |
| format | Artículo |
| id | INTA21500 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA215002025-02-28T10:39:06Z Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 Kornuta, Claudia Alejandra Bidart, Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Tribulatti, María Virginia Campetella, Oscar Prato, Cecilia A. Carabelli, Julieta Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines Langellotti, Cecilia Ana Herpesviridae Vaccine Adjuvants Immune Response Bovinae Animal Viruses Vaccines Coadyuvante de Vacunas Respuesta Inmunológica Virus de los Animales Vacuna Murine Model Modelo Murino Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide™ GEL01 PR (GEL01) and introduced Galectin-8 (Gal-8), a lectin considered a novel adjuvant due to its immunostimulatory effects, into the formulation. Animals were vaccinated with pCIgD, pCIgD with Gal-8 (pCIgD-Gal-8), pCIgD with Gal-8 and GEL01 (pCIgD-Gal-8-GEL01), or the control plasmid pCIneo. The immune response was first assessed in a mouse model and then in bovines. The results showed that combining Gal-8 and GEL01 with pCIgD modulated immune responses at both the humoral and cellular levels in both animal models. This study evaluates the efficacy of a DNA vaccine with Gal-8 and GEL01 as potential adjuvants to enhance immune protection against BoAHV-1. Instituto de Virología Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Kornuta, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Bidart, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Tribulatti, María Virginia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Tribulatti, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campetella, Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campetella, Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Prato, Cecilia A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Prato, Cecilia A. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Carabelli, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carabelli, Julieta. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Cheuquepan Valenzuel, Felipe Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Cheuquepan Valenzuel, Felipe Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore. Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Moore. Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Moore. Dadín Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-02-28T10:17:06Z 2025-02-28T10:17:06Z 2025-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21500 https://www.sciencedirect.com/science/article/abs/pii/S0042682225000145 1096-0341 https://doi.org/10.1016/j.virol.2025.110402 eng info:eu-repograntAgreement/INTA/PNBIO/1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Virology 604 : 110402 (March 2025) |
| spellingShingle | Herpesviridae Vaccine Adjuvants Immune Response Bovinae Animal Viruses Vaccines Coadyuvante de Vacunas Respuesta Inmunológica Virus de los Animales Vacuna Murine Model Modelo Murino Kornuta, Claudia Alejandra Bidart, Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Tribulatti, María Virginia Campetella, Oscar Prato, Cecilia A. Carabelli, Julieta Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines Langellotti, Cecilia Ana Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 |
| title | Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 |
| title_full | Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 |
| title_fullStr | Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 |
| title_full_unstemmed | Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 |
| title_short | Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1 |
| title_sort | galectin 8 and gel01 as potential adjuvants to enhance the immune response induced by a dna vaccine against bovine alphaherpesvirus type 1 |
| topic | Herpesviridae Vaccine Adjuvants Immune Response Bovinae Animal Viruses Vaccines Coadyuvante de Vacunas Respuesta Inmunológica Virus de los Animales Vacuna Murine Model Modelo Murino |
| url | http://hdl.handle.net/20.500.12123/21500 https://www.sciencedirect.com/science/article/abs/pii/S0042682225000145 https://doi.org/10.1016/j.virol.2025.110402 |
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