Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography
Peanut (Arachis hypogaea L.), a major source of oil, is the second most widely cultivated legume crop worldwide after soybean. Argentina is the largest producer of confectionery peanuts in Latin America and the seventh in the world. This legume has bioactive compounds of nutritional value, phytoster...
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| Format: | Artículo |
| Language: | Inglés |
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Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21250 https://revistas.unc.edu.ar/index.php/agris/article/view/44246 https://doi.org/10.31047/1668.298x.v41.n2.44246 |
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| author | Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José |
| author_browse | Aquilano, Claudio Gabriel Martínez, María José Percibaldi, Nora Mabel |
| author_facet | Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José |
| author_sort | Aquilano, Claudio Gabriel |
| collection | INTA Digital |
| description | Peanut (Arachis hypogaea L.), a major source of oil, is the second most widely cultivated legume crop worldwide after soybean. Argentina is the largest producer of confectionery peanuts in Latin America and the seventh in the world. This legume has bioactive compounds of nutritional value, phytosterols among the most important ones. Phytosterols protect against cancer and lower cholesterol rates. The objective of this work was to adjust and refine a method to identify and quantify phytosterol content in peanut seeds by gas chromatography. The method was found to be accurate, as demonstrated through repeatability and reproducibility tests, and expressed by the coefficients of variation. No significant differences were found between the results obtained and the reference values using a confidence level of 95 % and 5 degrees of freedom. The results obtained in this work presented 44.31 mg of beta-sitosterol and 3.39 mg of stigmasterol per 100 g of roasted peanuts, in agreement with the values reported in the literature. |
| format | Artículo |
| id | INTA21250 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba |
| publisherStr | Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba |
| record_format | dspace |
| spelling | INTA212502025-02-13T13:49:39Z Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography Implementación de un método para la identificación y cuantificación de fitoesteroles en semillas de maní (Arachis hypogaea L.) por cromatografía gaseosa Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José Arachis hypogaea Cacahuete Esteroles Colesterol Estigmasterol Espectrometría de Masas Groundnuts Sterols Cholesterol Stigmasterol Mass Spectrometry Maní Beta-sitosterol Peanut (Arachis hypogaea L.), a major source of oil, is the second most widely cultivated legume crop worldwide after soybean. Argentina is the largest producer of confectionery peanuts in Latin America and the seventh in the world. This legume has bioactive compounds of nutritional value, phytosterols among the most important ones. Phytosterols protect against cancer and lower cholesterol rates. The objective of this work was to adjust and refine a method to identify and quantify phytosterol content in peanut seeds by gas chromatography. The method was found to be accurate, as demonstrated through repeatability and reproducibility tests, and expressed by the coefficients of variation. No significant differences were found between the results obtained and the reference values using a confidence level of 95 % and 5 degrees of freedom. The results obtained in this work presented 44.31 mg of beta-sitosterol and 3.39 mg of stigmasterol per 100 g of roasted peanuts, in agreement with the values reported in the literature. El maní (Arachis hypogaea L.), una importante fuente de aceite, es la segunda leguminosa más cultivada en todo el mundo después de la soja. Argentina es el mayor productor de maní confitero de América Latina y el séptimo del mundo. Esta leguminosa posee compuestos bioactivos y los fitoesteroles son uno de los más importantes. Los fitoesteroles protegen contra el cáncer y reducen el colesterol. El objetivo de este trabajo fue ajustar y mejorar un método para identificar y cuantificar el contenido de fitoesteroles en semillas de maní mediante cromatografía de gases. Se concluyó que el método era preciso, como lo demuestran las pruebas de repetibilidad y reproducibilidad expresadas por los coeficientes de variación. No se encontraron diferencias significativas entre los resultados obtenidos y los valores de referencia utilizando un nivel de confianza del 95 % y cinco grados de libertad. Los resultados obtenidos en este trabajo, correspondiente a 44,31 mg de beta-sitosterol y 3,39 mg de estigmasterol por 100 g de maní tostado, concuerdan con los informados en la literatura. EEA Pergamino Fil: Aquilano, Claudio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos Suelos y Agua; Argentina Fil: Percibaldi, Nora Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Aguas; Argentina Fil: Martínez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina 2025-02-13T13:38:28Z 2025-02-13T13:38:28Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21250 https://revistas.unc.edu.ar/index.php/agris/article/view/44246 1668-298X (online) https://doi.org/10.31047/1668.298x.v41.n2.44246 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba AgriScientia 41 (2) : 19-26. (diciembre 2024) |
| spellingShingle | Arachis hypogaea Cacahuete Esteroles Colesterol Estigmasterol Espectrometría de Masas Groundnuts Sterols Cholesterol Stigmasterol Mass Spectrometry Maní Beta-sitosterol Aquilano, Claudio Gabriel Percibaldi, Nora Mabel Martínez, María José Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title | Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_full | Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_fullStr | Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_full_unstemmed | Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_short | Implementation of a method for the identification and quantification of phytosterols in peanut (Arachis hypogaea L.) by gas chromatography |
| title_sort | implementation of a method for the identification and quantification of phytosterols in peanut arachis hypogaea l by gas chromatography |
| topic | Arachis hypogaea Cacahuete Esteroles Colesterol Estigmasterol Espectrometría de Masas Groundnuts Sterols Cholesterol Stigmasterol Mass Spectrometry Maní Beta-sitosterol |
| url | http://hdl.handle.net/20.500.12123/21250 https://revistas.unc.edu.ar/index.php/agris/article/view/44246 https://doi.org/10.31047/1668.298x.v41.n2.44246 |
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