Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs
Toxoplasma gondii is a protozoan parasite causing toxoplasmosis, a principal concern for public health and livestock industries. Effective vaccination strategies are crucial for controlling toxoplasmosis, particularly in the lamb, which are significant reservoirs of T. gondii. In addition, ovine tox...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21236 https://www.sciencedirect.com/science/article/abs/pii/S0001706X25000208 https://doi.org/10.1016/j.actatropica.2025.107540 |
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| author | Campero, Lucia Maria Gual, Ignacio Sander, Valeria Mendoza Morales, Luisa Ramos Duarte, Victor Formigo, Paula Sosa, Emiliano Lázaro, Fermín Scioli, Maria Valeria Atela, Agustín Legarralde, Ariel Hozbor, Federico Andres Canton, German Jose Angel, Sergio Moore, Prando Dadin Clemente, Marina |
| author_browse | Angel, Sergio Atela, Agustín Campero, Lucia Maria Canton, German Jose Clemente, Marina Formigo, Paula Gual, Ignacio Hozbor, Federico Andres Legarralde, Ariel Lázaro, Fermín Mendoza Morales, Luisa Moore, Prando Dadin Ramos Duarte, Victor Sander, Valeria Scioli, Maria Valeria Sosa, Emiliano |
| author_facet | Campero, Lucia Maria Gual, Ignacio Sander, Valeria Mendoza Morales, Luisa Ramos Duarte, Victor Formigo, Paula Sosa, Emiliano Lázaro, Fermín Scioli, Maria Valeria Atela, Agustín Legarralde, Ariel Hozbor, Federico Andres Canton, German Jose Angel, Sergio Moore, Prando Dadin Clemente, Marina |
| author_sort | Campero, Lucia Maria |
| collection | INTA Digital |
| description | Toxoplasma gondii is a protozoan parasite causing toxoplasmosis, a principal concern for public health and livestock industries. Effective vaccination strategies are crucial for controlling toxoplasmosis, particularly in the lamb, which are significant reservoirs of T. gondii. In addition, ovine toxoplasmosis also causes economic losses due to abortions and reproductive complications. In this study, we evaluated two immunization strategies to elucidate the immune protective potential of T. gondi major surface protein SAG1 fused to the plant heat shock proteins 90-kDa (pHsp90) adjuvant against experimental toxoplasmosis in lambs. We performed an oral administration of fresh leaves homogenate infiltrated with a B- and T-cell antigenic epitope-containing surface protein SAG1 (SAG1HC) fused to Arabidopsis thaliana Hsp90 (AtHsp81.2-SAG1HC) (Plant Vaccine) and a subcutaneous administration of recombinant SAG1HC fused to Nicotiana benthamiana Hsp90 (NbHsp90.3-SAG1HC) produced in Escherichia coli (Recombinant Vaccine). Our results showed that only the Recombinant Vaccine significantly increased anti-rSAG1 total IgG values (∼ 4-fold more than the Vehicle and Control groups). In addition, only lambs immunized with the Plant Vaccine showed a significant increase (∼ 3-fold more than the Vehicle and Control groups) in IFN-γ serum levels after the experimental infection (evaluated 8 days post-challenge). On the other hand, we also observed a statistically significant decrease (∼ 80 % less) in histopathological lesions (injury score) in challenged vaccinated lambs compared to challenged but not vaccinated animals (Vehicle and Control groups). Previously, we showed that the chimera recombinant Gra4-Gra7 protein is an acute marker of human infection. Since Gra4-Gra7 is not connected to the SAG1 immunogen, this chimera allows us to monitor infection in challenged lambs early. All lambs from the Control and Vehicle groups showed higher rates of serological reactivity than lambs from the vaccinated groups, concurrently with increased severity of lesions. These results suggest that the Plant-based and Recombinant Vaccines are promising candidates for controlling T. gondii infection in lambs, with potential benefits for enhancing public health and animal welfare. |
| format | Artículo |
| id | INTA21236 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA212362025-02-13T10:16:43Z Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs Campero, Lucia Maria Gual, Ignacio Sander, Valeria Mendoza Morales, Luisa Ramos Duarte, Victor Formigo, Paula Sosa, Emiliano Lázaro, Fermín Scioli, Maria Valeria Atela, Agustín Legarralde, Ariel Hozbor, Federico Andres Canton, German Jose Angel, Sergio Moore, Prando Dadin Clemente, Marina Toxoplasmosis Ewes Vaccines Immune Response Immunization Lambs Oveja Vacuna Respuesta Inmunológica Inmunización Cordero Toxoplasma gondii is a protozoan parasite causing toxoplasmosis, a principal concern for public health and livestock industries. Effective vaccination strategies are crucial for controlling toxoplasmosis, particularly in the lamb, which are significant reservoirs of T. gondii. In addition, ovine toxoplasmosis also causes economic losses due to abortions and reproductive complications. In this study, we evaluated two immunization strategies to elucidate the immune protective potential of T. gondi major surface protein SAG1 fused to the plant heat shock proteins 90-kDa (pHsp90) adjuvant against experimental toxoplasmosis in lambs. We performed an oral administration of fresh leaves homogenate infiltrated with a B- and T-cell antigenic epitope-containing surface protein SAG1 (SAG1HC) fused to Arabidopsis thaliana Hsp90 (AtHsp81.2-SAG1HC) (Plant Vaccine) and a subcutaneous administration of recombinant SAG1HC fused to Nicotiana benthamiana Hsp90 (NbHsp90.3-SAG1HC) produced in Escherichia coli (Recombinant Vaccine). Our results showed that only the Recombinant Vaccine significantly increased anti-rSAG1 total IgG values (∼ 4-fold more than the Vehicle and Control groups). In addition, only lambs immunized with the Plant Vaccine showed a significant increase (∼ 3-fold more than the Vehicle and Control groups) in IFN-γ serum levels after the experimental infection (evaluated 8 days post-challenge). On the other hand, we also observed a statistically significant decrease (∼ 80 % less) in histopathological lesions (injury score) in challenged vaccinated lambs compared to challenged but not vaccinated animals (Vehicle and Control groups). Previously, we showed that the chimera recombinant Gra4-Gra7 protein is an acute marker of human infection. Since Gra4-Gra7 is not connected to the SAG1 immunogen, this chimera allows us to monitor infection in challenged lambs early. All lambs from the Control and Vehicle groups showed higher rates of serological reactivity than lambs from the vaccinated groups, concurrently with increased severity of lesions. These results suggest that the Plant-based and Recombinant Vaccines are promising candidates for controlling T. gondii infection in lambs, with potential benefits for enhancing public health and animal welfare. EEA Balcarce Fil: Campero, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Sander, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Instituto Tecnológico de Chascomús; Argentina Fil: Sander, Valeria. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil. Mendoza Morales, Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Instituto Tecnológico de Chascomús; Argentina Fil. Mendoza Morales, Luisa. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil: Ramos Duarte, Victor. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil: Ramos Duarte, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Laboratorio de Molecular Farming y Vacunas; Argentina Fil: Formigo, Paula. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil: Formigo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Laboratorio de Parasitología Molecular; Argentina Fil: Sosa, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Lázaro, Fermín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Atela, Agustín. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil: Atela, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Laboratorio de Molecular Farming y Vacunas; Argentina Fil: Legarralde, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Laboratorio de Molecular Farming y Vacunas; Argentina Fil: Hozbor, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Angel, Sergio. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil: Angel, Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Laboratorio de Parasitología Molecular; Argentina Fil: Moore, Dadín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Clemente, Marina. Universidad Nacional General San Martin. Escuela de Bio y Nanotecnologías; Argentina Fil: Clemente, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional General San Martin; Argentina. Laboratorio de Molecular Farming y Vacunas; Argentina 2025-02-13T10:04:54Z 2025-02-13T10:04:54Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21236 https://www.sciencedirect.com/science/article/abs/pii/S0001706X25000208 1873-6254 (Online) https://doi.org/10.1016/j.actatropica.2025.107540 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Acta Tropica 262 : 107540. (February 2025) |
| spellingShingle | Toxoplasmosis Ewes Vaccines Immune Response Immunization Lambs Oveja Vacuna Respuesta Inmunológica Inmunización Cordero Campero, Lucia Maria Gual, Ignacio Sander, Valeria Mendoza Morales, Luisa Ramos Duarte, Victor Formigo, Paula Sosa, Emiliano Lázaro, Fermín Scioli, Maria Valeria Atela, Agustín Legarralde, Ariel Hozbor, Federico Andres Canton, German Jose Angel, Sergio Moore, Prando Dadin Clemente, Marina Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs |
| title | Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs |
| title_full | Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs |
| title_fullStr | Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs |
| title_full_unstemmed | Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs |
| title_short | Immunization with plant-based vaccine expressing Toxoplasma gondii SAG1 fused to plant HSP90 elicits protective immune response in lambs |
| title_sort | immunization with plant based vaccine expressing toxoplasma gondii sag1 fused to plant hsp90 elicits protective immune response in lambs |
| topic | Toxoplasmosis Ewes Vaccines Immune Response Immunization Lambs Oveja Vacuna Respuesta Inmunológica Inmunización Cordero |
| url | http://hdl.handle.net/20.500.12123/21236 https://www.sciencedirect.com/science/article/abs/pii/S0001706X25000208 https://doi.org/10.1016/j.actatropica.2025.107540 |
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